Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,635,542 A→G H157H (CAT→CAC)  der ← predicted GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,635,5420AG99.2% 365.8 / NA 126H157H (CAT→CAC) derpredicted GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (125/0);  minor base C (1/0);  total (126/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CGGTGCGAGATCTTCCATCCACGGCAGCAGCACATGCTCCAGCAGACTTAATACGCCACGACC  >  W3110S.gb/2635509‑2635571
                                 |                             
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTg                             >  1:1116906/1‑36 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCa       >  1:212706/1‑58 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGa    >  1:1383405/1‑61 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGa    >  1:1574316/1‑61 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:558624/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:584760/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:555569/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:554180/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:552984/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:551400/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:545405/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:538937/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:529212/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:524592/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:486997/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:466759/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:420098/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:38708/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:378769/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:371563/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:369663/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:361551/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:351758/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:337416/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:265378/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:231109/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:825974/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:977274/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:972066/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:96978/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:95857/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:956121/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:929898/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:925198/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:886413/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:875248/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:873520/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:870597/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:832681/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:570666/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:777698/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:764910/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:758489/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:715852/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:705658/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:670517/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:606441/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:597644/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:596149/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:589475/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:201404/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1164616/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:204413/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1317402/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1307081/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1292377/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:126372/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1262976/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1249108/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1235491/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1202544/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1189357/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1172965/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1325030/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1162610/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1157757/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1144549/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1119325/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:108055/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1079624/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:105978/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:105414/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1027452/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:102212/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1018660/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1521049/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1000236/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:200680/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:200380/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:184060/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1647533/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1644602/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1628667/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1617391/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1583019/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1581355/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1550239/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1356514/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1520720/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1514590/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1508879/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1456121/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1447359/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1416893/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1405410/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1381988/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1362916/1‑62 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTC‑‑‑CAGACTTAATACGCCACGAcc  >  1:490155/1‑60 (MQ=255)
cGGTGCGAGATCTTCCATCCACGGCAGCACCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:1293580/1‑62 (MQ=255)
cGGAGCGAGATCTTCCATCCACGGCAGCAGCACGTGCTCCAGCAGACTTAATACGCCACGAc   >  1:110129/1‑62 (MQ=255)
                                 |                             
CGGTGCGAGATCTTCCATCCACGGCAGCAGCACATGCTCCAGCAGACTTAATACGCCACGACC  >  W3110S.gb/2635509‑2635571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A