Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,973,864 C→A A269A (GCG→GCT)  aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,973,8640CA100.0% 553.1 / NA 195A269A (GCG→GCT) aasfused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (195/0);  total (195/0)

Only 100 of 195 total aligned reads displayed.

CCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTC  >  W3110S.gb/2973850‑2973911
              |                                               
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTa                     >  1:1325142/1‑43 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGc      >  1:1438266/1‑58 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCtt   >  1:686536/1‑61 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCtt   >  1:728835/1‑61 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCtt   >  1:907455/1‑61 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCtt   >  1:865265/1‑61 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCtt   >  1:1457457/1‑61 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCt    >  1:1231225/1‑60 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:444254/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:595025/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:565637/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:558628/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:553358/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:550725/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:530848/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:511624/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:462192/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:199515/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:415208/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:400248/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:375968/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:365895/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:358004/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:286751/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:284523/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:253920/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:231098/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:202917/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:170699/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:825323/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:994417/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:95965/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:953011/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:941887/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:918329/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:890744/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:887390/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:86138/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:839655/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:828760/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:826826/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:602502/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:824323/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:804001/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:79186/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:77577/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:766701/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:746749/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:73675/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:692248/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:656912/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:630556/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1175059/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:192875/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1347017/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1333868/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1300814/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1293548/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1290476/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1284542/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1277004/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1263800/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1215079/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1190046/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1354818/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1149071/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1139181/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1130138/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1111013/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1102744/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1092684/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:106795/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1033014/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1022891/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1013605/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1519896/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1009018/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1630332/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1622542/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1604942/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1592563/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1575847/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1548162/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1543016/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1359106/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1513145/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1494347/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1488574/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1467397/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1427363/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1406500/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:140131/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1389702/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1369253/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1367772/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1365120/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGACTTc  >  1:312776/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATCCGTTCGCCTTc  >  1:1017230/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTGATGCCTGCATTGGGCAGCATTAAGCCCATGCGTTCGCCTTc  >  1:1097950/1‑62 (MQ=255)
ccAAAAATCACTGCAGCACTCATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTc  >  1:1554712/1‑62 (MQ=255)
              |                                               
CCAAAAATCACTGCCGCACTGATGCCTGCATTGGGCAGCATTAAGCCGATGCGTTCGCCTTC  >  W3110S.gb/2973850‑2973911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A