Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,056,662 G→A H135H (CAC→CAT)  serA ← D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,056,6620GA100.0% 583.6 / NA 204H135H (CAC→CAT) serAD‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (204/0);  total (204/0)

Only 100 of 204 total aligned reads displayed.

CCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAT  >  W3110S.gb/3056657‑3056718
     |                                                        
ccACGATGCGCTTTAGCATTGTCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1539662/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCgcgc                         >  1:278384/1‑39 (MQ=38)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATa                     >  1:390120/1‑43 (MQ=38)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAgcagcagca            >  1:1068577/1‑52 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGt          >  1:851462/1‑54 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCTAAt  >  1:334992/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCaa   >  1:320933/1‑61 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:508507/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:670602/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:631032/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:626752/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:600904/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:595079/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:583740/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:57255/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:566380/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:557298/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:544733/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:537601/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:518634/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:405450/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:499869/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:39477/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:485651/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:469085/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:468060/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:458593/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:420131/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:816678/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:983993/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:973151/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:961985/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:95121/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:934478/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:920476/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:911368/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:893994/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:86770/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:84637/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:84235/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:82910/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:634464/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:809178/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:794318/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:779474/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:75927/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:719272/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:716011/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:713783/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:693906/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:685931/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:675200/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:35124/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1190336/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1456725/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1427485/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1422662/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1393833/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1361475/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1355835/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1347778/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1300759/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:128629/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1247587/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1209978/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1513191/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1182402/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1176258/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1151717/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1141251/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1124171/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:110247/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:105055/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1029089/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1019179/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1002886/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:189344/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1000107/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:349474/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:337989/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:322655/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:314279/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:287383/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:274160/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:261188/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:24205/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:200972/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:383246/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:184151/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:17688/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1649408/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1632323/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1621423/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1583336/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1568343/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1548630/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1541389/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:1526092/1‑62 (MQ=255)
ccACGATGCGCTTTAGCATTGGCTTCTCCCACGCCccgc                         >  1:130780/1‑39 (MQ=16)
ccACGATGCGCTTTAGAATTGGCTTCTGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAt  >  1:41005/1‑62 (MQ=255)
     |                                                        
CCACGGTGCGCTTTAGCATTGGCTTCCGGCACGCCGCGCAATAGCAGCAGCAGTTCGCCAAT  >  W3110S.gb/3056657‑3056718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A