Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,233,557 G→A H325H (CAC→CAT)  ygjO ← predicted methyltransferase small domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,233,5570GA100.0% 644.0 / NA 224H325H (CAC→CAT) ygjOpredicted methyltransferase small domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (224/0);  total (224/0)

Only 100 of 225 total aligned reads displayed.

CAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAG  >  W3110S.gb/3233514‑3233575
                                           |                  
cAACGATATACAGCTCGCCGTTGATTTTCCGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:1459304/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGa             >  1:926694/1‑51 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAAc           >  1:57677/1‑53 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACAtct       >  1:375031/1‑57 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACAtct       >  1:64145/1‑57 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTccc    >  1:1551388/1‑60 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTccc    >  1:439278/1‑60 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTccc    >  1:394845/1‑60 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:1064513/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:974979/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:1480241/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:612117/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:103134/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:204835/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:682911/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:781809/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:1307299/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:140007/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCa   >  1:1332349/1‑61 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:633448/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:620422/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:58761/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:553696/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:582467/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:570261/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:356282/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:52057/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:509634/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:487718/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:47987/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:459962/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:436293/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:408170/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:316308/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:827503/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:997900/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:993456/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:960268/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:950844/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:933898/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:887691/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:861137/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:855169/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:831908/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:648446/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:821859/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:81336/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:794986/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:766664/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:752234/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:725112/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:711247/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:655655/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:651845/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1244378/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1470857/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:144176/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1414215/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1375850/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1368977/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1324296/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1305340/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:13030/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1290896/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1270027/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1249057/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1505637/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1239215/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1234145/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1193481/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1177842/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1176934/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1165552/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1078231/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1059079/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1017680/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:101122/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1509601/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:335177/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:330171/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1005321/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:312028/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:280372/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:265457/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:260469/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:237018/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:201359/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:200503/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:188591/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1643936/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1635379/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1630085/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1625603/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:158888/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1575744/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:1535242/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:348142/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGAAACATCTCCCAg  >  1:494132/1‑62 (MQ=255)
cAACGATATACAGCTCGCCGTTGATATTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:543537/1‑62 (MQ=39)
cAACGATATACAGATCGCCGTTGATTTTCAGGCAGCGGCGGGCATGGTGGAACATCTCCCAg  >  1:29601/1‑62 (MQ=255)
                                           |                  
CAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTGGAACATCTCCCAG  >  W3110S.gb/3233514‑3233575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A