Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,242,729 G→A T223T (ACC→ACT)  uxaC ← uronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,242,7290GA100.0% 298.9 / NA 105T223T (ACC→ACT) uxaCuronate isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (105/0);  total (105/0)

Only 100 of 105 total aligned reads displayed.

GACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTT  >  W3110S.gb/3242688‑3242741
                                         |            
gACACTTTACATCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:35/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGATGTTTAGTCAGGGCAGtt  >  1:636408/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAg    >  1:658685/1‑52 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAg    >  1:1343418/1‑52 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:497888/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:620022/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:59293/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:591542/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:57966/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:552393/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:538441/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:534598/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:529551/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:505551/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:500921/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:32143/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:442853/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:434017/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:420650/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:417829/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:412331/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:382840/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:339673/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:325115/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1003397/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:3220/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:858789/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:993560/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:985401/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:976081/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:946597/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:945325/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:915881/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:908257/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:896829/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:883928/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:874789/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:865537/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:655368/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:816894/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:809316/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:748372/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:740817/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:720211/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:718549/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:698964/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:693894/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:683245/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:669195/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1380309/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:151723/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1516273/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1507842/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1502235/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1480632/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1477667/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1470553/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1431871/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1426649/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:140062/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1395919/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:138668/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1545339/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1351209/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1302087/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1291154/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1261927/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1244922/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1210443/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1205942/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1136562/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1136546/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1084842/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:100730/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:211932/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:301175/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:286253/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:277677/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:276934/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:26613/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:247453/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:238910/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:213781/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:315938/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:200932/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:194449/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:189255/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:178303/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1636134/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1624042/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:159616/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1593643/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1573007/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:1551081/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:319368/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGt   >  1:362850/1‑53 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGTCAGGGCAGt   >  1:292608/1‑53 (MQ=255)
gACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTAGCCAGGGCAGtt  >  1:393426/1‑54 (MQ=39)
gACACTTTACAGCCGTGAGCGGCGAAGTGATACAGACGTTTAGTCAGGGCAGtt  >  1:646286/1‑54 (MQ=255)
gACACTTTACAGCCGTGAGCGGAGAAGTGATCCAGACGTTTAGTCAGGGCAGtt  >  1:48740/1‑54 (MQ=255)
                                         |            
GACACTTTACAGCCGTGAGCGGCGAAGTGATCCAGACGTTTGGTCAGGGCAGTT  >  W3110S.gb/3242688‑3242741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A