Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,618,821 A→G N176N (AAT→AAC)  rmuC ← predicted recombination limiting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,618,8210AG100.0% 659.7 / NA 223N176N (AAT→AAC) rmuCpredicted recombination limiting protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (223/0);  total (223/0)

Only 100 of 223 total aligned reads displayed.

TGGAGATTGCGAATTTCGTGGGTCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTC  >  W3110S.gb/3618816‑3618877
     |                                                        
tGGAGGTTGCGGATTTCGTGAGTCATGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:563926/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTAcca          >  1:1648842/1‑53 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAATCTGTc  >  1:1571675/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGt   >  1:1383962/1‑61 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGt   >  1:182083/1‑61 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGt   >  1:795454/1‑61 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGt   >  1:777309/1‑61 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:409008/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:57211/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:54676/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:531319/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:505096/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:478263/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:45016/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:443658/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:416750/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:413865/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:33174/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:404528/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:400554/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:392853/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:387124/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:376214/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:364884/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:358366/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:354517/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:350985/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:259292/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:790271/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:976190/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:969658/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:921911/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:907089/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:901777/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:895009/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:883341/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:868757/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:837467/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:820838/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:807749/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:601340/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:786580/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:781954/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:756714/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:747340/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:717151/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:711063/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:698368/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:677404/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:656199/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:635702/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:621280/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1219133/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:277611/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1349630/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1329518/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1322510/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1318944/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1313944/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1277102/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1274926/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1258585/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1248378/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1236277/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1366491/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1187165/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1173767/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1155476/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1150517/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1124874/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1100283/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:107889/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1063646/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1050354/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1033285/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1014745/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1593766/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:268540/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1000719/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:248504/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:212365/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:197059/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:188786/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1643514/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1628650/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1615422/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1609106/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1372905/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1588968/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1583526/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:152866/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:150408/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1486115/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1469019/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1439769/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1432818/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1425933/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGTGAGTCAGGGTATGACGTTCTAGGGCTTCTTTACCAAAGCTGTc  >  1:1295210/1‑62 (MQ=255)
tGGAGGTTGCGGATTTCGAGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:241592/1‑62 (MQ=255)
tGGAGGTTGCGGATTTAGTGAGTCAGGGTATGACGTTCTTGGGCTTCTTTACCAAAGCTGTc  >  1:1406253/1‑62 (MQ=255)
     |                                                        
TGGAGATTGCGAATTTCGTGGGTCAGGGTATGGCGTTCTTGTGCTTCTTTACCGAAGCTGTC  >  W3110S.gb/3618816‑3618877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A