Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R2
|
489 |
0.0 |
1652083 |
58.3% |
963164 |
55.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,138,010 |
G→T |
S23R (AGC→AGA) |
frlC ← |
predicted isomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,138,010 | 0 | G | T | 100.0%
| 139.9
/ NA
| 50 | S23R (AGC→AGA) | frlC | predicted isomerase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (50/0); total (50/0) |
AGCTCGCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAT > W3110S.gb/4138005‑4138066
|
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCa > 1:845493/1‑37 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCtg > 1:501838/1‑44 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCtg > 1:187530/1‑44 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACa > 1:910848/1‑61 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACa > 1:789291/1‑61 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACa > 1:677322/1‑61 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACa > 1:1411173/1‑61 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:695132/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:365393/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:460074/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:523105/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:588415/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:597747/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:627116/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:640900/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:647843/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:322491/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:704191/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:752625/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:768992/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:772478/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:809378/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:829787/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:900920/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:96370/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:972026/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1383896/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1027543/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1113242/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1128284/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1130048/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:119001/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1196069/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1226105/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1239641/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:131783/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1323072/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1347303/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1397664/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1403689/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1463932/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1550284/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1581903/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1629798/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:221730/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:249693/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:283516/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:287829/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:1013508/1‑62 (MQ=255)
aGCTCTCTTGCATCACGAAATGCGTGTACAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAt > 1:326990/1‑62 (MQ=255)
|
AGCTCGCTTGCATCACGAAATGCGTGTTCAATAGGCAGCCGCTGGTGGCCGCAGGTAAACAT > W3110S.gb/4138005‑4138066
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
