Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,368,298 A→G L475L (CTT→CTC)  dipZ ← fused thiol:disulfide interchange protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,368,2980AG100.0% 652.3 / NA 221L475L (CTT→CTC) dipZfused thiol:disulfide interchange protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/221);  total (0/221)

Only 100 of 221 total aligned reads displayed.

CGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAAAGATCTAAC  >  W3110S.gb/4368246‑4368307
                                                    |         
cccTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1479831/60‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCTTAGAGATCTAAc  <  1:1039389/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:377627/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:561761/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:558855/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:55023/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:533666/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:492856/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:492042/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:481464/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:466365/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:444126/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:437682/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:402143/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:595167/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:367331/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:345468/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:322692/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:312720/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:307564/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:272930/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:26889/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:23472/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:232126/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:221638/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:215427/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:777519/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:982401/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:964651/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:959059/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:937591/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:923066/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:886312/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:883621/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:876867/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:866439/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:826824/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:815998/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:796491/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:567061/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:76903/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:759277/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:72552/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:701968/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:690928/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:688199/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:661368/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:648268/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:633878/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:629142/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:212101/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1191030/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1398837/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1372764/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1346434/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1334735/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1309838/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1299878/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1280902/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1277321/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1256529/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1240301/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1233179/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1213259/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1401235/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1141495/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1132584/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1130582/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1122510/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1102508/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1071194/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1068856/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1057934/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1022450/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1014267/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1007148/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1553675/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:207915/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:204857/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:173002/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1630064/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1626523/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1606503/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1596368/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1587720/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:156865/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1000960/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1535772/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1520460/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1514490/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1505455/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1495943/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1462410/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:144731/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1435091/62‑1 (MQ=255)
cGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:14080/62‑1 (MQ=255)
 gCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:254649/61‑1 (MQ=255)
 gCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1423355/61‑1 (MQ=255)
 gCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:1411937/61‑1 (MQ=255)
  cTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAGAGATCTAAc  <  1:171497/60‑1 (MQ=255)
                                                    |         
CGCTGAAGGTGTATTTCTCAAACTCTTTACAGGCGACGCACCAGTCGGCATAAAGATCTAAC  >  W3110S.gb/4368246‑4368307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A