Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R2 489 0.0 1652083 58.3% 963164 55.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,611,926 +A coding (457/1032 nt) rsmC ← 16S RNA m2G1207 methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,611,9231.A100.0% 233.4 / NA 82D154Y (GAT→TAT) rsmC16S RNA m2G1207 methylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/82);  total (0/82)

GTATTCGCCCCAGAATTTCTCCGCATC‑AAATACCGGCTGTTTTTCCAGACG  >  W3110S.gb/4611897‑4611947
                           |                        
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:348735/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:548238/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:530803/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:528769/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:521825/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:464360/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:449910/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:447865/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:435292/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:413675/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:375504/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:597371/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:311433/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:289140/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:270284/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:259515/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:251659/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:247777/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:244438/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1631814/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1006844/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:858204/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:97265/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:92030/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:919436/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:898774/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:897144/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:889602/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:887630/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:885228/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:870662/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1629229/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:824870/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:769636/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:764177/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:758197/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:674162/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:649604/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:63079/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:620445/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:612787/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1173787/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1628980/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1293927/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1279084/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1261632/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:124789/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1233303/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1230596/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1193528/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1179265/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1300899/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1167359/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1142608/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1136985/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1107880/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1056972/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1046675/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1039805/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1033120/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1014506/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1323537/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1623254/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1613650/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1607136/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1545607/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1539695/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1485890/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1483483/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:147955/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1466965/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1460482/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:146037/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1452121/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1436980/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1429690/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1406339/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1343427/52‑1 (MQ=39)
gTATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1301183/52‑1 (MQ=39)
gTATTCGCCACAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1262137/52‑1 (MQ=255)
 tATTCGCCCCAGAATTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:1175572/51‑1 (MQ=39)
              aTTTCTCCGCATCAAAATACCGGCTGTTTTTCCAgacg  <  1:207701/38‑1 (MQ=37)
                           |                        
GTATTCGCCCCAGAATTTCTCCGCATC‑AAATACCGGCTGTTTTTCCAGACG  >  W3110S.gb/4611897‑4611947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A