Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 58 121.6 3649812 78.1% 2850503 56.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,306,558 A→G I346I (ATT→ATC)  yeeF ← predicted amino‑acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,306,5580AG100.0% 363.4 / NA 96I346I (ATT→ATC) yeeFpredicted amino‑acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/71);  total (25/71)

ACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTT  >  minE/1306529‑1306581
                             |                       
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAggc         <  1:1617517/46‑2 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2788197/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3346192/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3313746/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3119336/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3072420/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2941155/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2906489/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2884969/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2872660/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3471189/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2771088/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1000959/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2721615/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2634456/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2629583/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2560391/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2557582/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2555830/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2539400/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:562654/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:976840/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:917051/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:908877/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:83737/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:761522/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:746826/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:738783/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:724201/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:713324/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3447201/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:405944/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:388680/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3577462/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3563981/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3549986/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3535324/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:3505073/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2757781/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1458080/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1878265/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1785080/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1677269/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1620848/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:158837/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1519294/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1517498/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1505556/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2472508/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:126488/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1232870/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1220371/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1177798/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:116050/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1157074/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1085803/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1072146/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2512343/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2357735/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1904023/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2346252/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2246276/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2228379/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:222142/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:219045/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2167034/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2110028/46‑1 (MQ=255)
aCCAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2050949/46‑1 (MQ=255)
 ccAGGTCGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1215905/45‑1 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:2100278/39‑1 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGga         <  1:1157115/39‑1 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3420225/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:1879033/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:950464/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:934128/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:309053/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3155407/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3264337/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:328258/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:29105/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:1782200/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:2652368/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:711692/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:1977400/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:53775/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:481242/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:1393040/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:1730753/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3619109/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3448719/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3002871/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:2865611/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3456865/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3534444/1‑46 (MQ=255)
       cGAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:2045504/1‑46 (MQ=255)
       agAAGTTGATTGCCAGCAGGGCGATCGCGCCAACCAGGATGATGtt  >  1:3178500/2‑46 (MQ=39)
                             |                       
ACCAGGTCGAAGTTGATTGCCAGCAGGGCAATCGCGCCAACCAGGATGATGTT  >  minE/1306529‑1306581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A