Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 58 121.6 3649812 78.1% 2850503 56.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,314,633 A→G E296E (GAA→GAG)  hisB → fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,314,6330AG100.0% 356.6 / NA 93E296E (GAA→GAG) hisBfused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (93/0);  total (93/0)

CCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAAT  >  minE/1314574‑1314635
                                                           |  
ccGCACCTGGAATATAAAGCTGAATTTACCTTCCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:974078/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGTCGATCTCAGCACCGAGAt  >  1:2538187/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3139563/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3524440/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3493689/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3490519/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3468209/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3375658/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3366321/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3365716/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:334166/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:329362/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3288457/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3157839/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3548729/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3112294/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3059339/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2988948/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2965991/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2913616/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2901698/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2799826/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:278490/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2783467/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2780917/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:618417/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1035357/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:955398/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:948501/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:934175/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:927464/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:836308/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:800438/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:76659/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:750340/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:691065/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:645214/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2615169/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:525561/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:524077/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:508904/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:467381/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:448387/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:395996/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:392160/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:376889/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3565802/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:3555959/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1206108/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1649151/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1637461/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1594130/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1527082/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1523967/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:149991/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1450445/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1388390/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1312/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1280150/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1222289/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1694834/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1200622/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1182299/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:115656/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1145081/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:114091/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1130443/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1128959/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1124680/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1076734/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1074824/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2007438/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:103207/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2481915/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2385542/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2308821/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2234674/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2224764/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2146541/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:207488/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:2054641/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:204537/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1054549/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1922309/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1866641/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1840884/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1826777/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1822687/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1819638/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1767138/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1758433/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1724099/1‑62 (MQ=255)
ccGCACCTGGAATATAAAGCTGAATTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAGAt  >  1:1718207/1‑62 (MQ=255)
                                                           |  
CCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAAT  >  minE/1314574‑1314635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A