Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 58 121.6 3649812 78.1% 2850503 56.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,663,103 A→G E31G (GAA→GGA)  yfgI → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,663,1030AG100.0% 360.0 / NA 94E31G (GAA→GGA) yfgIconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (94/0);  total (94/0)

CTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGA  >  minE/1663087‑1663148
                |                                             
ctctctGCTGTCGCAGGATCGGGACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:343780/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTTAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1408241/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATg                        >  1:3202160/1‑40 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGc                  >  1:942488/1‑46 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACg   >  1:2918654/1‑61 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACg   >  1:1599439/1‑61 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3072000/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2818349/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:342974/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3315005/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3267687/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3250575/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2814588/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:315371/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3150555/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3123765/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3082823/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2888770/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3034506/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3020021/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2987182/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2950789/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2859024/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2932336/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2913677/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2891583/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:524627/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:842983/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:817385/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:670585/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:652667/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:62835/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:617468/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:599061/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:579504/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:569821/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:524799/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3444891/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:515394/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:481929/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:378070/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3586936/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3579743/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3563947/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3547523/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:3492179/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:347350/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1621360/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1118994/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1907137/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1847242/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1846763/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1786255/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1766915/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1766585/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1758302/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1753878/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1711871/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1627994/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1912001/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1617313/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1476283/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1448596/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1392024/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1389868/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:133860/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1317544/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1147064/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1136231/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1127305/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2372150/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2812805/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:277721/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2774413/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2768801/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2699283/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2556774/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2506184/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2494543/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2422103/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1922827/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2354658/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2333412/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:216766/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2145314/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2042092/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2026699/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2001665/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1988522/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:1960058/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGAATGACGa  >  1:1388080/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGAAGAATGACGa  >  1:3352791/1‑62 (MQ=255)
ctctctGCTGTCGCAGGATCGGAACAGCAAGGTAAAAAAGAAGAGCAAAGGCAGCAAGACg   >  1:365423/1‑61 (MQ=255)
ctctctGCTGTAGCAGGATCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGa  >  1:2634202/1‑62 (MQ=255)
                |                                             
CTCTCTGCTGTCGCAGAAGCGGAACAGCAAGGTAAAAATGAAGAGCAAAGGCAGCATGACGA  >  minE/1663087‑1663148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A