Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R1
|
58 |
121.6 |
3649812 |
78.1% |
2850503 |
56.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,256,042 |
T→C |
K332K (AAA→AAG) |
rpoC ← |
RNA polymerase, beta prime subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,256,042 | 0 | T | C | 100.0%
| 16.5
/ NA
| 6 | K332K (AAA→AAG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/0); total (6/0) |
GTAGTCAACACGCTTACCGAGCAGGTTCTGACGGAAACGACCCTGTTTACCTTTGATCATGT > minE/2255991‑2256052
|
gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:1077132/1‑60 (MQ=255)
gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:1090279/1‑60 (MQ=255)
gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:2136741/1‑60 (MQ=255)
gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:2905414/1‑60 (MQ=255)
gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:3165138/1‑60 (MQ=255)
gtagtCGACGCGCTTACCCAGCAAGTTTTGACGGAAACGACCGCCCTTGCCCTTGATCAttt > 1:3440336/1‑60 (MQ=255)
|
GTAGTCAACACGCTTACCGAGCAGGTTCTGACGGAAACGACCCTGTTTACCTTTGATCATGT > minE/2255991‑2256052
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
