Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
835,160 |
A→C |
8.6% |
A1206A (GCA→GCC) |
narG → |
nitrate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 835,160 | 0 | A | C | 8.6%
| 200.2
/ 13.8
| 81 | A1206A (GCA→GCC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (41/33); new base C (5/2); total (46/35) |
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
ATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGA > minE/835092‑835227
|
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa > 1:1251060/1‑71 (MQ=255)
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa > 1:2098608/1‑71 (MQ=255)
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa > 1:429898/1‑71 (MQ=255)
aTCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATa > 1:3549546/1‑71 (MQ=255)
tCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATAc < 1:3102207/71‑1 (MQ=255)
cATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACgg < 1:3298958/71‑1 (MQ=255)
tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt < 1:1131923/71‑1 (MQ=255)
tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt < 1:1992560/71‑1 (MQ=255)
tAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt > 1:3395370/1‑71 (MQ=255)
aaCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt > 1:2771878/1‑71 (MQ=255)
aaCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt > 1:3048541/1‑71 (MQ=255)
aCTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCtt > 1:1526720/1‑70 (MQ=255)
ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACg > 1:2633978/1‑62 (MQ=255)
ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACg > 1:716266/1‑62 (MQ=255)
ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac < 1:681022/70‑1 (MQ=255)
ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac > 1:1438096/1‑70 (MQ=255)
ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac > 1:1998371/1‑70 (MQ=255)
ggTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAac > 1:314025/1‑70 (MQ=255)
gTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAacta < 1:1524965/71‑1 (MQ=255)
gTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAacta < 1:566995/71‑1 (MQ=255)
tCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAactac > 1:2711088/1‑71 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACTGCTTTAActacta < 1:1967627/71‑1 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:3653860/63‑1 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCttt < 1:1847513/63‑1 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCt < 1:2014893/61‑1 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta > 1:491929/1‑71 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta > 1:3344492/1‑71 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta > 1:3427113/1‑71 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta > 1:2688957/1‑71 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta < 1:992946/71‑1 (MQ=255)
aCCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAActacta > 1:1197218/1‑71 (MQ=255)
cccGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTAt < 1:2930574/71‑1 (MQ=255)
gTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGc < 1:26367/71‑1 (MQ=255)
aCGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGt < 1:3088007/71‑1 (MQ=255)
cGCCGAAACGCACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTa < 1:3688406/71‑1 (MQ=255)
cGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTa < 1:2263758/71‑1 (MQ=255)
gCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAg < 1:3084032/71‑1 (MQ=255)
ccGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAgg > 1:530452/1‑71 (MQ=255)
ccGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAgg > 1:3466709/1‑71 (MQ=255)
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg > 1:909239/1‑60 (MQ=255)
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg > 1:2442846/1‑60 (MQ=255)
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg > 1:2168642/1‑60 (MQ=255)
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATg > 1:3650211/1‑60 (MQ=255)
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt < 1:109088/71‑1 (MQ=255)
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt < 1:1185876/71‑1 (MQ=255)
gAAACCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCgg > 1:408821/1‑69 (MQ=255)
aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGGACCGTCggat > 1:1525433/1‑68 (MQ=255)
aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGt > 1:24556/1‑65 (MQ=255)
aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc > 1:1549236/1‑71 (MQ=255)
aaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc > 1:2450242/1‑70 (MQ=255)
aCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt > 1:2220365/1‑70 (MQ=255)
aCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATcga < 1:816707/71‑3 (MQ=255)
ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa < 1:355606/71‑1 (MQ=255)
aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg > 1:2983578/1‑71 (MQ=255)
aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg > 1:604286/1‑71 (MQ=255)
cGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc < 1:1218457/71‑1 (MQ=255)
gCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgcg < 1:2561273/71‑1 (MQ=255)
atatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGAt < 1:200674/71‑1 (MQ=255)
atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:98069/1‑71 (MQ=255)
atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:3215670/1‑71 (MQ=255)
atGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:3049761/1‑69 (MQ=255)
tGATCGGCGGCTATGCCCATCTGGCATACGGCTTTaa > 1:3154818/1‑37 (MQ=255)
aTCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGtt < 1:2785975/71‑1 (MQ=255)
ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt < 1:3327877/71‑1 (MQ=255)
ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:1556131/1‑71 (MQ=255)
cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3675487/71‑1 (MQ=255)
ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:3690880/71‑1 (MQ=255)
ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:2664677/71‑1 (MQ=255)
ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:1734870/71‑1 (MQ=255)
gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:2177215/1‑69 (MQ=255)
gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:2686193/1‑69 (MQ=255)
aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg > 1:727580/1‑71 (MQ=255)
tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATg > 1:1602763/1‑56 (MQ=255)
tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3122389/65‑1 (MQ=255)
cATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt > 1:2342998/1‑69 (MQ=255)
aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt > 1:3067849/1‑68 (MQ=255)
tGGCATACGGCTTTAACTACTATGGCACCGTAGGtt > 1:131592/1‑36 (MQ=255)
tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAg > 1:804932/1‑68 (MQ=255)
gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCTATGAGTTTGTTGTAGTGCGTAAGATga < 1:3546/70‑1 (MQ=255)
gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCTATGAGTTTGTTGTAGTGCGTAAGATga < 1:1801173/70‑1 (MQ=255)
gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg < 1:1718797/69‑1 (MQ=255)
|
ATCCATAACTCGGTCACCCGTATCACGCCGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGA > minE/835092‑835227
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
