Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
835,190 |
T→A |
5.6% |
G1216G (GGT→GGA) |
narG → |
nitrate reductase 1, alpha subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 835,190 | 0 | T | A | 5.6%
| 249.7
/ 4.1
| 86 | G1216G (GGT→GGA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (31/50); new base A (3/2); total (34/52) |
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
CGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAT > minE/835120‑835259
|
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt < 1:109088/71‑1 (MQ=255)
cGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGt < 1:1185876/71‑1 (MQ=255)
aaaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc > 1:1549236/1‑71 (MQ=255)
aaCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATc > 1:2450242/1‑70 (MQ=255)
aCCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCt > 1:2220365/1‑70 (MQ=255)
aCCAACGCATATGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATcga < 1:816707/71‑3 (MQ=255)
ccGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTaa < 1:355606/71‑1 (MQ=255)
aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg > 1:604286/1‑71 (MQ=255)
aCGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcg > 1:2983578/1‑71 (MQ=255)
cGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgc < 1:1218457/71‑1 (MQ=255)
gCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACcgcg < 1:2561273/71‑1 (MQ=255)
atatGATTGGCGGTTACGCGCAGCTGGCCTGGGGCTTTAACTACTACGGCACCGTCGGATCGAACCGCGAt < 1:200674/71‑1 (MQ=255)
atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:3215670/1‑71 (MQ=255)
atatGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:98069/1‑71 (MQ=255)
atGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGAt > 1:3049761/1‑69 (MQ=255)
aTCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGtt < 1:2785975/71‑1 (MQ=255)
ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt < 1:3327877/71‑1 (MQ=255)
ggcggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgt > 1:1556131/1‑71 (MQ=255)
cggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3675487/71‑1 (MQ=255)
ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:3690880/71‑1 (MQ=255)
ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:2664677/71‑1 (MQ=255)
ggcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTtgttgt < 1:1734870/71‑1 (MQ=255)
gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:2686193/1‑69 (MQ=255)
gcTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg > 1:2177215/1‑69 (MQ=255)
aTGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTg > 1:727580/1‑71 (MQ=255)
tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATg > 1:1602763/1‑56 (MQ=255)
tGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTttgttg < 1:3122389/65‑1 (MQ=255)
cATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt > 1:2342998/1‑69 (MQ=255)
aTCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt > 1:3067849/1‑68 (MQ=255)
tGGCATACGGCTTTAACTACTATGGCACCGTAGGtt > 1:131592/1‑36 (MQ=255)
tGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAg > 1:804932/1‑68 (MQ=255)
gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCTATGAGTTTGTTGTAGTGCGTAAGATga < 1:1801173/70‑1 (MQ=255)
gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCTATGAGTTTGTTGTAGTGCGTAAGATga < 1:3546/70‑1 (MQ=255)
gCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATg < 1:1718797/69‑1 (MQ=255)
tACGGCTTTAACTACTATGGCACCGTAGGTTCTAAcc < 1:1314845/37‑1 (MQ=255)
tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:3550476/1‑71 (MQ=255)
tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa > 1:2276106/1‑71 (MQ=255)
tACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaag < 1:1816253/69‑1 (MQ=255)
cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt > 1:114608/1‑54 (MQ=255)
cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:2503628/70‑1 (MQ=255)
cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:878151/71‑1 (MQ=255)
cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa < 1:266581/71‑1 (MQ=255)
ggCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAt > 1:3114466/1‑71 (MQ=255)
tttAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATga < 1:3452892/61‑1 (MQ=255)
tttAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATga < 1:2844417/61‑1 (MQ=255)
tAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGtt > 1:2389628/1‑39 (MQ=255)
tAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt > 1:3390692/1‑71 (MQ=255)
tAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt > 1:99290/1‑71 (MQ=255)
tAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt > 1:454501/1‑71 (MQ=255)
tAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt > 1:1697763/1‑71 (MQ=255)
actactATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTg < 1:3275510/70‑1 (MQ=255)
ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt > 1:423842/1‑71 (MQ=255)
ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGAt < 1:603014/51‑1 (MQ=255)
ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt > 1:2625679/1‑61 (MQ=255)
aTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa > 1:3218340/1‑52 (MQ=255)
aTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa > 1:529662/1‑52 (MQ=255)
aTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa > 1:2611098/1‑52 (MQ=255)
aTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa > 1:3437243/1‑52 (MQ=255)
aTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt > 1:88283/1‑71 (MQ=255)
tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGtt > 1:287978/1‑66 (MQ=255)
tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:1213042/70‑1 (MQ=255)
tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:569843/70‑1 (MQ=255)
tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:2075129/70‑1 (MQ=255)
tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:3485146/70‑1 (MQ=255)
tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt < 1:827336/70‑1 (MQ=255)
cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:2329966/55‑1 (MQ=255)
cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:1223131/55‑1 (MQ=255)
cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:3669895/55‑1 (MQ=255)
cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt < 1:1195937/55‑1 (MQ=255)
cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:1364874/71‑1 (MQ=255)
cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg < 1:1307624/71‑1 (MQ=255)
aCCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCgt < 1:1304904/71‑2 (MQ=255)
aCCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa < 1:2420994/71‑1 (MQ=255)
ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATga < 1:1307605/45‑1 (MQ=255)
cGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg > 1:1478731/1‑49 (MQ=255)
aGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGc < 1:3497147/70‑1 (MQ=255)
ggTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa < 1:1697250/71‑1 (MQ=255)
ggTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCa < 1:2755581/70‑1 (MQ=255)
ggTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCa < 1:1669195/70‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:172213/44‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:1200367/44‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAAc < 1:2434660/44‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGa < 1:3604605/49‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1355086/56‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1274157/56‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1023939/56‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa < 1:1032409/56‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt < 1:406690/71‑1 (MQ=255)
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt < 1:2146333/71‑1 (MQ=255)
|
CGAAACCGACGCATATGATCGGCGGCTATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAT > minE/835120‑835259
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
