Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,014,719 |
T→C |
5.5% |
Q450Q (CAA→CAG) ‡ |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,014,719 | 0 | T | C | 5.5%
| 272.9
/ 4.7
| 91 | Q450Q (CAA→CAG) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (51/35); new base C (3/2); total (54/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCG > minE/1014651‑1014786
|
gCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTg < 1:1115746/71‑1 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:256349/1‑71 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:688963/1‑71 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCACCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1528744/1‑70 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTAGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:1630231/1‑71 (MQ=255)
tCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:3298645/1‑71 (MQ=255)
tCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:2316786/1‑71 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCg < 1:3010248/70‑1 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGa < 1:96148/71‑1 (MQ=255)
tCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGtt < 1:1729560/63‑1 (MQ=255)
cACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACa > 1:1543197/1‑70 (MQ=255)
aCCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACAt > 1:3553830/1‑70 (MQ=255)
aCCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCAcg > 1:1696151/1‑68 (MQ=255)
tgctgGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGa > 1:3612077/1‑64 (MQ=255)
tgctgGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:1654041/1‑70 (MQ=255)
tgctgGCTGCGGTTGCCTTTCGCCAGCATGATCAAACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg > 1:2290286/1‑70 (MQ=255)
ctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGagt > 1:1372482/1‑69 (MQ=255)
ctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGagt > 1:3391991/1‑69 (MQ=255)
gctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa < 1:3372384/55‑1 (MQ=255)
gctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa < 1:3376674/55‑1 (MQ=255)
gctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatcc < 1:1438703/70‑1 (MQ=255)
cctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCTCCCTGCGCTTGCAGTTGATCGACATAAGAatcca < 1:2763591/70‑1 (MQ=255)
ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa < 1:1423252/54‑1 (MQ=255)
ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:354527/67‑1 (MQ=255)
ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat < 1:2274862/67‑1 (MQ=255)
tgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:516534/1‑55 (MQ=255)
tgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATc > 1:2548659/1‑55 (MQ=255)
tgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatc > 1:2617295/1‑71 (MQ=255)
gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtc < 1:2894135/71‑1 (MQ=255)
gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtc < 1:3149736/71‑1 (MQ=255)
ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGAc < 1:666455/53‑1 (MQ=255)
accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc < 1:897784/59‑1 (MQ=255)
accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc < 1:3541972/59‑1 (MQ=255)
ccTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGtt < 1:1483595/43‑1 (MQ=255)
ccTTTCGCCAGCATGATCATACTTCCGCCCTGCCCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg < 1:3420205/70‑1 (MQ=255)
cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa < 1:2405443/56‑1 (MQ=255)
cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa < 1:262842/56‑1 (MQ=255)
cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcggc < 1:1962246/70‑1 (MQ=255)
ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATaa > 1:3639728/1‑51 (MQ=255)
ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATaa > 1:1834072/1‑51 (MQ=255)
ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt > 1:1426070/1‑71 (MQ=255)
ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt > 1:3312496/1‑71 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:2633440/1‑41 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:3512581/1‑41 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:3466974/1‑41 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1579359/1‑41 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:2329721/1‑41 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:1988346/1‑71 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:2096966/1‑71 (MQ=255)
tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc > 1:2218883/1‑71 (MQ=255)
gCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1852286/1‑71 (MQ=255)
ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGtt < 1:1223378/71‑1 (MQ=255)
cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:3237529/1‑71 (MQ=255)
cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:1549984/71‑1 (MQ=255)
cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:1977890/71‑1 (MQ=255)
cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:849755/71‑1 (MQ=255)
cAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCAATCCGTCCGGCGGTCGTTg > 1:3627355/1‑71 (MQ=255)
gCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt > 1:1118202/1‑67 (MQ=255)
cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc < 1:765202/71‑1 (MQ=255)
aTGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc < 1:2368238/71‑1 (MQ=255)
gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:2595611/1‑69 (MQ=255)
gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc > 1:1274646/1‑69 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt < 1:2606145/62‑1 (MQ=255)
aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAag < 1:2876656/71‑1 (MQ=255)
aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa < 1:664880/67‑1 (MQ=255)
tACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGaa < 1:1289561/71‑1 (MQ=255)
cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg > 1:1447578/1‑49 (MQ=255)
cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg > 1:1524483/1‑49 (MQ=255)
cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcgg > 1:1099523/1‑49 (MQ=255)
cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:763165/1‑61 (MQ=255)
cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2426018/1‑61 (MQ=255)
cTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc > 1:2532796/1‑61 (MQ=255)
cTTCCGCCCTGCGCTTGCAGTTGACCGACATAAGAATCCAtccgtccg > 1:1929395/1‑48 (MQ=255)
ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:3365301/1‑71 (MQ=255)
ttCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:2218762/1‑71 (MQ=255)
ccccccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:1393359/4‑69 (MQ=255)
ccGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg > 1:1079546/1‑55 (MQ=255)
ccGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg > 1:2116742/1‑69 (MQ=255)
cGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg < 1:110734/54‑1 (MQ=255)
cccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAgg > 1:3631389/1‑70 (MQ=255)
ccTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCa > 1:959778/1‑71 (MQ=255)
cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCAt < 1:2673727/71‑1 (MQ=255)
tGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCAt > 1:1849560/1‑70 (MQ=255)
tGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCAt > 1:2384967/1‑70 (MQ=255)
cTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCt < 1:1257919/71‑1 (MQ=255)
cTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCt < 1:3012474/71‑1 (MQ=255)
ttGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGa < 1:2219880/60‑1 (MQ=255)
tGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc < 1:2071370/46‑1 (MQ=255)
gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGaa > 1:680422/1‑53 (MQ=255)
gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGaa > 1:1068468/1‑53 (MQ=255)
gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc < 1:3379689/70‑1 (MQ=255)
gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc < 1:1647673/70‑1 (MQ=255)
cAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCg > 1:2766370/1‑71 (MQ=255)
ttGATCGTCATAAGAATCCATCCGTCCGGCGGTCGTTggg < 1:3111988/40‑1 (MQ=255)
|
GCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCG > minE/1014651‑1014786
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
