Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,153,878 |
G→A |
45.6% |
T246I (ACA→ATA) |
astE ← |
succinylglutamate desuccinylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,153,878 | 0 | G | A | 45.6%
| 22.6
/ 106.8
| 79 | T246I (ACA→ATA) | astE | succinylglutamate desuccinylase |
| Reads supporting (aligned to +/- strand): ref base G (22/21); new base A (18/18); total (40/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 |
CATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGC > minE/1153811‑1153947
|
cATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATtc > 1:2720425/1‑71 (MQ=255)
cATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATtc > 1:3486456/1‑71 (MQ=255)
tGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCAc < 1:607689/71‑1 (MQ=255)
cATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACg < 1:1630005/70‑1 (MQ=255)
cATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGa > 1:432727/1‑71 (MQ=255)
aTTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGAt < 1:3107051/71‑1 (MQ=255)
aTTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGa > 1:1922785/1‑70 (MQ=255)
aTTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGAt < 1:1300862/71‑1 (MQ=255)
aTTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGAt < 1:1146240/71‑1 (MQ=255)
aTTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGAt < 1:1137475/71‑1 (MQ=255)
aTTTCGAAGGAAGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGa > 1:925829/1‑70 (MQ=255)
ttCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATAc > 1:3070669/1‑71 (MQ=255)
ttCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATAc > 1:2015075/1‑71 (MQ=255)
gAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGtt < 1:3104667/58‑1 (MQ=255)
gAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGa > 1:985784/1‑65 (MQ=255)
gAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGa < 1:492245/71‑1 (MQ=255)
aGGACGGCGAGTGGTGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGaca < 1:271005/71‑1 (MQ=255)
aGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGaca < 1:1019491/71‑1 (MQ=255)
aGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGaca < 1:2406912/71‑1 (MQ=255)
aGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGaca < 1:2402476/71‑1 (MQ=255)
cGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACActct < 1:54508/71‑1 (MQ=255)
cGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGACActct > 1:1753793/1‑71 (MQ=255)
ggcgagtggcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACActct < 1:3438282/70‑1 (MQ=255)
gcgagtggcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCa < 1:2712961/71‑1 (MQ=255)
agtggcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCa < 1:3363246/71‑1 (MQ=255)
gtggcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCAc > 1:2426186/1‑68 (MQ=255)
gcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGa > 1:1655916/1‑69 (MQ=255)
gcgagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGAt < 1:2006867/70‑1 (MQ=255)
gagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCa < 1:1508493/65‑1 (MQ=255)
gagtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCa < 1:2219395/65‑1 (MQ=255)
gtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGAt > 1:838691/1‑66 (MQ=255)
gtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATagcag > 1:1734701/1‑71 (MQ=255)
gtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATagcag > 1:2517005/1‑71 (MQ=255)
gtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATagcag > 1:2510578/1‑71 (MQ=255)
gtAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATagcag > 1:991243/1‑71 (MQ=255)
aaTTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAgcagcg > 1:1501475/1‑71 (MQ=255)
aTTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAgcagcg > 1:1850574/1‑70 (MQ=255)
aTTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACg > 1:3085540/1‑42 (MQ=255)
aTTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAgcagcg > 1:2419537/1‑70 (MQ=255)
aTTTGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAgcagcg > 1:2570814/1‑70 (MQ=255)
tGCGAAACCACCCGATAACGGAGCGGCGGTATTCTCACAATACCGACACTCTCACCAGATAgcagcgcag > 1:1966904/1‑70 (MQ=255)
cGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACAct > 1:2413955/1‑48 (MQ=255)
cGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACAct > 1:493184/1‑48 (MQ=255)
cGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACAct > 1:3338587/1‑48 (MQ=255)
cGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCaa > 1:1555830/1‑71 (MQ=255)
aaaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAgcagcgcagc < 1:697404/67‑1 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGAt < 1:1010850/55‑1 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCaa < 1:385443/68‑1 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTg > 1:1254349/1‑71 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTg > 1:126337/1‑71 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTg > 1:1636204/1‑71 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTg > 1:2205948/1‑71 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTg > 1:2124961/1‑71 (MQ=255)
aaCCACCCGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATagcagc < 1:2637034/61‑1 (MQ=255)
accaccCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTgc > 1:99074/1‑71 (MQ=255)
accaccCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTg > 1:681534/1‑70 (MQ=255)
accCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCt < 1:3714247/71‑1 (MQ=255)
accCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCt < 1:347577/71‑1 (MQ=255)
accCGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCt > 1:751606/1‑71 (MQ=255)
ccGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg < 1:3203250/71‑1 (MQ=255)
ccGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg > 1:576213/1‑71 (MQ=255)
ccGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg < 1:2726170/71‑1 (MQ=255)
ccGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg < 1:27871/71‑1 (MQ=255)
cGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg > 1:3486641/1‑70 (MQ=255)
cGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg > 1:3038191/1‑70 (MQ=255)
cGATAACGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg > 1:1153988/1‑70 (MQ=255)
gATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTgg > 1:99300/1‑69 (MQ=255)
aTAACGGAGCGGCGGTGTTCTCACGATACCGacac > 1:2917554/1‑35 (MQ=255)
aCGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTa < 1:3422530/71‑1 (MQ=255)
aCGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTa < 1:1883824/71‑1 (MQ=255)
aCGGAGCGGCGGTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTa < 1:2085062/71‑1 (MQ=255)
ggAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACt < 1:781234/71‑1 (MQ=255)
cggcggTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCa < 1:1876993/70‑1 (MQ=255)
gcggTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAAc < 1:556467/71‑1 (MQ=255)
gcggTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAAc < 1:1153300/71‑1 (MQ=255)
cggTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACt < 1:108859/71‑1 (MQ=255)
ggTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTg < 1:3170080/71‑1 (MQ=255)
ggTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTg < 1:1293302/71‑1 (MQ=255)
ggTATTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTg < 1:971817/71‑1 (MQ=255)
tgtTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTgg > 1:23382/1‑70 (MQ=255)
tgtTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGc > 1:3032328/1‑71 (MQ=255)
tgtTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGc > 1:201821/1‑71 (MQ=255)
gtTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGc > 1:3441403/1‑70 (MQ=255)
|
CATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGATAACGGAGCGGCGGTGTTCTCACGATACCGACACTCTCACCAGATAGCAGCGCAGCAATTGCGCTGGCAGTTACTGCAAACTGGC > minE/1153811‑1153947
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
