Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F21 I0 R2	370	72.1	3738297	91.5%	3420541	62.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,246,787	C→T	59.1%	G70G (GGC→GGT)	znuC →	zinc transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,246,787	0	C	T	59.1%	34.7 / 79.3	66	G70G (GGC→GGT)	znuC	zinc transporter subunit
Reads supporting (aligned to +/- strand): ref base C (16/11); new base T (16/23); total (32/34)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

TACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTT  >  minE/1246717‑1246852

tACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGc                                                                   <  1:1469516/71‑1 (MQ=255)

 aCGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGc                                                                   >  1:229549/1‑70 (MQ=255)

  cGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTa                                                                 >  1:842117/1‑71 (MQ=255)

   gggTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTAt                                                                >  1:2421605/1‑71 (MQ=255)

    ggTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATg                                                               <  1:3397748/71‑1 (MQ=255)

    ggTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATg                                                               <  1:964335/71‑1 (MQ=255)

    ggTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATg                                                               <  1:592642/71‑1 (MQ=255)

     gTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGt                                                              <  1:394029/71‑1 (MQ=255)

     gTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGt                                                              <  1:389566/71‑1 (MQ=255)

     gTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGt                                                              <  1:2213201/71‑1 (MQ=255)

     gTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGt                                                              <  1:2104519/71‑1 (MQ=255)

           cTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCg                                                          >  1:1773825/1‑69 (MQ=255)

                 cTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCt                                                  <  1:3330847/71‑1 (MQ=255)

                   ggTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTg                                                 >  1:613794/1‑70 (MQ=255)

                   ggTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTg                                                 >  1:1714440/1‑70 (MQ=255)

                   ggTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTg                                                 >  1:2851991/1‑70 (MQ=255)

                    gTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTa                                               >  1:1205692/1‑71 (MQ=255)

                    gTAACACCCGATCAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGt                                                <  1:1776191/70‑1 (MQ=255)

                        cacCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTAtctc                                           <  1:3301369/71‑1 (MQ=255)

                         acCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTAtctc                                           >  1:3183471/1‑70 (MQ=255)

                          cccGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGa                                         <  1:1069112/71‑1 (MQ=255)

                           ccGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTAt                                              >  1:1064411/1‑65 (MQ=255)

                            cGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGaca                                       >  1:187879/1‑71 (MQ=255)

                             gATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGacac                                      >  1:307972/1‑71 (MQ=255)

                                gAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGAcaccac                                   >  1:1359465/1‑71 (MQ=255)

                                 aaGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACg                                  <  1:2423336/71‑1 (MQ=255)

                                          tCAAGCGCAGCGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACt                          <  1:1347061/70‑1 (MQ=255)

                                          tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGt                                 <  1:484814/63‑1 (MQ=255)

                                          tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGt                                 <  1:216463/63‑1 (MQ=255)

                                          tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGt                                 <  1:2726531/63‑1 (MQ=255)

                                          tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACt                          <  1:3482309/70‑1 (MQ=255)

                                          tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACt                          <  1:536669/70‑1 (MQ=255)

                                          tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACt                          <  1:1988327/70‑1 (MQ=255)

                                          tCAAGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTg                         <  1:2454261/71‑1 (MQ=255)

                                            aaGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGa                        <  1:3425672/70‑1 (MQ=255)

                                             aGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGAcc                      >  1:2405257/1‑71 (MQ=255)

                                             aGCGCAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGAc                       >  1:1679221/1‑70 (MQ=255)

                                               cgcAACGGAAAACTGCTCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGt                    <  1:3426700/71‑1 (MQ=255)

                                                gcAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGAcc                      <  1:44488/68‑1 (MQ=255)

                                                gcAACGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGAcc                      <  1:123984/68‑1 (MQ=255)

                                                gcAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGt                    >  1:405703/1‑70 (MQ=255)

                                                gcAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTa                   <  1:3534100/71‑1 (MQ=255)

                                                    cGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAAcc               >  1:3525787/1‑71 (MQ=255)

                                                    cGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAAcc               >  1:715521/1‑71 (MQ=255)

                                                    cGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAAcc               >  1:2069950/1‑71 (MQ=255)

                                                    cGGAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCAAGTTGCCACTGACCGTAAAcc               >  1:3550296/1‑71 (MQ=255)

                                                     ggAAAACTGCGCATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCg              >  1:754314/1‑71 (MQ=255)

                                                        aaaCTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGacac                                      <  1:2965837/44‑1 (MQ=255)

                                                        aaaCTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGacac                                      <  1:950605/44‑1 (MQ=255)

                                                            tGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTtta       >  1:1912939/1‑71 (MQ=255)

                                                                cATCTGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct   <  1:1259374/71‑1 (MQ=255)

                                                                cATCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct   <  1:3593601/71‑1 (MQ=255)

                                                                cATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgc    <  1:1501075/70‑1 (MQ=255)

                                                                 aTCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct   <  1:2502800/70‑1 (MQ=255)

                                                                 aTCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaa                  >  1:1276348/1‑55 (MQ=255)

                                                                 aTCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaa                  >  1:2086450/1‑55 (MQ=255)

                                                                 aTCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaa                  >  1:3643215/1‑55 (MQ=255)

                                                                 aTCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaa                  >  1:267963/1‑55 (MQ=255)

                                                                 aTCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgctt  <  1:1134068/71‑1 (MQ=255)

                                                                  tCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGcc                             <  1:721706/43‑1 (MQ=255)

                                                                  tCGGTTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGcc                             <  1:1781777/43‑1 (MQ=255)

                                                                    ggCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct   >  1:383020/1‑67 (MQ=255)

                                                                    ggCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct   >  1:2453742/1‑67 (MQ=255)

                                                                    ggCTATGTACAGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTttacgct   >  1:91584/1‑67 (MQ=255)

                                                                     gCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaaa                 >  1:127323/1‑52 (MQ=255)

                                                                     gCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaaa                 >  1:1881632/1‑52 (MQ=255)

                                                                     gCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaaa                 >  1:1848215/1‑52 (MQ=255)

                                                                     gCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTaaa                 >  1:2948777/1‑52 (MQ=255)

                                                                      cTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGAc                       >  1:940333/1‑45 (MQ=255)

TACGGGTAGTGCTCGGGCTGGTAACACCCGATGAAGGGGTTATCAAGCGCAACGGAAAACTGCGCATCGGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTT  >  minE/1246717‑1246852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A