Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,522,743 |
T→C |
63.4% |
I121V (ATC→GTC) |
pdxB ← |
erythronate‑4‑phosphate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,522,743 | 0 | T | C | 63.4%
| 79.8
/ 82.9
| 82 | I121V (ATC→GTC) | pdxB | erythronate‑4‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (17/13); new base C (32/20); total (49/33) |
| Fisher's exact test for biased strand distribution p-value = 8.15e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATAT > minE/1522674‑1522813
|
gTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATc < 1:751492/71‑1 (MQ=255)
aGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATcc > 1:3318087/1‑69 (MQ=255)
ttAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCg > 1:320595/1‑70 (MQ=255)
tAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGt < 1:1622867/70‑1 (MQ=255)
aTCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTAc < 1:1299862/70‑1 (MQ=255)
tCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACgg < 1:2168172/71‑1 (MQ=255)
ccTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGt < 1:1636741/70‑1 (MQ=255)
cTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTc > 1:1882886/1‑70 (MQ=255)
cTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCg > 1:2665838/1‑71 (MQ=255)
tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCg > 1:495690/1‑70 (MQ=255)
tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCAcc > 1:3275752/1‑61 (MQ=255)
tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTc > 1:1145192/1‑69 (MQ=255)
tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCg > 1:3243338/1‑70 (MQ=255)
tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCg > 1:3069666/1‑70 (MQ=255)
tAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCg > 1:2561306/1‑70 (MQ=255)
aaCGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGt > 1:568325/1‑70 (MQ=255)
cGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACa < 1:3503444/71‑1 (MQ=255)
cGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACa < 1:3507739/71‑1 (MQ=255)
cGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACa < 1:1935525/71‑1 (MQ=255)
ccAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGa > 1:705251/1‑70 (MQ=255)
cAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGaaa < 1:1140828/71‑1 (MQ=255)
cAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGaaa < 1:3484455/71‑1 (MQ=255)
cAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaa < 1:1669727/70‑1 (MQ=255)
cAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGaa < 1:3050053/70‑1 (MQ=255)
tCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGt < 1:3311565/51‑1 (MQ=255)
tCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATc > 1:2797662/1‑71 (MQ=255)
cgcgCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATc > 1:1061870/1‑70 (MQ=255)
cgcgCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATc > 1:2074269/1‑70 (MQ=255)
gcgcCTGCAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCa > 1:681693/1‑71 (MQ=255)
cTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCa < 1:1572102/67‑1 (MQ=255)
cTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCa < 1:1790274/67‑1 (MQ=255)
cTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATcgc > 1:2998631/1‑71 (MQ=255)
gCAATCGACGGCAAACGTTACCAACGCCCACGACCCCCACCGTACGGt < 1:1543617/48‑1 (MQ=38)
cAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGtt > 1:280708/1‑71 (MQ=255)
cAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCAATCGCGt > 1:2551685/1‑70 (MQ=255)
cAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATcgcg < 1:850328/69‑1 (MQ=255)
cAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATcgcg < 1:1845685/69‑1 (MQ=255)
cAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGtt > 1:3204896/1‑71 (MQ=255)
cAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGtt > 1:488367/1‑71 (MQ=255)
cAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGtt > 1:1910991/1‑71 (MQ=255)
cAATCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGt < 1:654662/70‑1 (MQ=255)
aaTCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGtt < 1:3001286/70‑1 (MQ=255)
aTCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCAt > 1:2001598/1‑63 (MQ=255)
aTCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCAt > 1:1317100/1‑63 (MQ=255)
aTCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCAt > 1:1550723/1‑63 (MQ=255)
aTCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCg > 1:2926448/1‑71 (MQ=255)
aTCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCg > 1:1164373/1‑71 (MQ=255)
aTCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCg < 1:2193113/71‑1 (MQ=255)
tCGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCgg < 1:2445574/71‑1 (MQ=255)
cGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATc > 1:2536206/1‑58 (MQ=255)
cGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCgg > 1:2740491/1‑70 (MQ=255)
cGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCgg > 1:2661078/1‑70 (MQ=255)
cGACGTCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCgg > 1:5513/1‑70 (MQ=255)
tCCAACGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACa < 1:572312/44‑1 (MQ=255)
cAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCAt > 1:3588069/1‑70 (MQ=255)
aCGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:1623102/1‑71 (MQ=255)
aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATc > 1:2284928/1‑49 (MQ=255)
aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag < 1:833647/71‑1 (MQ=255)
aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:99367/1‑71 (MQ=255)
aCGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:1715993/1‑71 (MQ=255)
cGTTACCAACGCCCACGATCCCCACCGTACGGTCGTa > 1:2080931/1‑37 (MQ=255)
cGTTACCAACGCCCACGATCCCCACCGTACGGTCGTa > 1:2545208/1‑37 (MQ=255)
cGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagc < 1:206198/71‑1 (MQ=255)
cGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:909817/1‑70 (MQ=255)
cGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:1874436/1‑70 (MQ=255)
cGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:520448/1‑70 (MQ=255)
cGTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcag > 1:1092070/1‑70 (MQ=255)
gTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagca < 1:215492/71‑1 (MQ=255)
gTTACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagc < 1:3441708/70‑1 (MQ=255)
ttACCACCGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagcag < 1:3200834/71‑1 (MQ=255)
tACCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCgg < 1:3011325/57‑1 (MQ=255)
aCCAACGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAgg < 1:886750/70‑1 (MQ=255)
ccAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGgga < 1:2629142/71‑1 (MQ=255)
ccAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGgga < 1:2151110/71‑1 (MQ=255)
aaCGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGggag > 1:3711207/1‑70 (MQ=255)
cGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGggag > 1:3413960/1‑68 (MQ=255)
cGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATc > 1:2180294/1‑39 (MQ=255)
cGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATc > 1:104781/1‑39 (MQ=255)
cGCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATc > 1:1509714/1‑39 (MQ=255)
gCCCACGACCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGgaggag > 1:3389707/1‑70 (MQ=255)
aCGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATcagca > 1:1849376/1‑58 (MQ=255)
cGACCCCCACCGTACGGTCGTACAGTGAAAATCCATcgcg < 1:977278/40‑1 (MQ=255)
tCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACatat < 1:2910933/71‑1 (MQ=255)
|
GTAAGGTTTTAATCCCTAACGCTTCCAGTCGCGCCTGCAATCGACGTCCAACGTTACCAACGCCCACGATCCCCACCGTACGGTCGTACAGTGAAAATCCATCGCGTTCGGCAAGCATCAGCAGGGAGGAGAAAACATAT > minE/1522674‑1522813
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
