Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,744,552 |
G→A |
65.9% |
V483I (GTC→ATC) |
nadB → |
quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,744,552 | 0 | G | A | 65.9%
| 46.4
/ 38.4
| 44 | V483I (GTC→ATC) | nadB | quinolinate synthase, L‑aspartate oxidase (B protein) subunit |
| Reads supporting (aligned to +/- strand): ref base G (7/8); new base A (21/8); total (28/16) |
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAAT > minE/1744482‑1744619
|
ccTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCcgcg > 1:1431314/1‑71 (MQ=255)
tGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:3113030/1‑71 (MQ=255)
ggAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtct > 1:2844447/1‑71 (MQ=255)
ggAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtct > 1:384045/1‑71 (MQ=255)
aaCGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtctc > 1:2879757/1‑70 (MQ=255)
aaCGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGtctc > 1:2102392/1‑70 (MQ=255)
gcgcCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCa < 1:1037959/68‑1 (MQ=255)
gcggcgGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGc > 1:1182794/1‑71 (MQ=255)
gcggcgGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGc > 1:3671317/1‑71 (MQ=255)
cggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTgg < 1:3255943/70‑1 (MQ=255)
ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg < 1:2610782/71‑1 (MQ=255)
ggATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAg < 1:1379496/71‑1 (MQ=255)
gATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCt < 1:3041324/66‑1 (MQ=255)
gATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCt < 1:557483/66‑1 (MQ=255)
aTAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGGTGGAGCt > 1:764018/1‑71 (MQ=255)
aTAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCt < 1:716536/71‑1 (MQ=255)
ccATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCAGCGTCTCAAATAATTTGCTGGAGCTGCg > 1:1862300/1‑70 (MQ=255)
aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:1627214/1‑45 (MQ=255)
aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:1827157/1‑45 (MQ=255)
aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:531361/1‑45 (MQ=255)
aTGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCAtc > 1:2171138/1‑45 (MQ=255)
tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTg > 1:3297185/1‑56 (MQ=255)
tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTg > 1:1308502/1‑56 (MQ=255)
tGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTg > 1:905483/1‑56 (MQ=255)
ccAACAAGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTgg > 1:2422103/1‑71 (MQ=255)
aacagGAAATATACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:2592818/1‑71 (MQ=255)
aacagGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:2588448/1‑71 (MQ=255)
aacaaGAAATAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTa > 1:273373/1‑71 (MQ=255)
caaGAAATAGACGAATATTACGCCCATTTCCGCATCTCAaataa > 1:748045/1‑44 (MQ=255)
caaGAAATAGACGAATATTACGCCCATTTCCGCATCTCAaataa > 1:394012/1‑44 (MQ=255)
aaaTAGACGAATATTACGCCCATTTCCACATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGtt < 1:1076744/71‑1 (MQ=255)
aaTAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTTCGTAATCTGGTACAGGtt < 1:3422933/70‑1 (MQ=255)
aTAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTg > 1:2980900/1‑70 (MQ=255)
aTAGACGAATATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGc > 1:327896/1‑71 (MQ=255)
gACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGa < 1:804734/71‑1 (MQ=255)
aaTATTACGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTg < 1:1043925/71‑1 (MQ=255)
ttACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGAtt < 1:1983171/70‑1 (MQ=255)
ttACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATATGGTACAGGTTGCCGAGTTGATTg > 1:108580/1‑71 (MQ=255)
cGCCCATTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGtt < 1:1080390/70‑1 (MQ=255)
gCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCg < 1:1815615/71‑1 (MQ=255)
aTTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt < 1:3629290/71‑1 (MQ=255)
aTTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt < 1:704719/71‑1 (MQ=255)
aTTTCCGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCtgt > 1:2590553/1‑71 (MQ=255)
tCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGc > 1:1450366/1‑70 (MQ=255)
ccGCATCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCaa > 1:1394544/1‑71 (MQ=255)
cgcaTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAat < 1:1687737/71‑1 (MQ=255)
cgcaTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAat < 1:2191954/71‑1 (MQ=255)
|
CCTGGAACGCGCCCTGCGGCGGATAACCATGCTCCAACAAGAAATAGACGAATATTACGCCCATTTCCGCGTCTCAAATAATTTGCTGGAGCTGCGTAATCTGGTACAGGTTGCCGAGTTGATTGTTCGCTGTGCAAT > minE/1744482‑1744619
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
