Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R2
|
370 |
72.1 |
3738297 |
91.5% |
3420541 |
62.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,463,313 |
G→A |
50.0% |
V200V (GTG→GTA) |
gidB → |
methyltransferase, glucose‑inhibited cell‑division protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,463,313 | 0 | G | A | 50.0%
| ‑1.3
/ 75.1
| 54 | V200V (GTG→GTA) | gidB | methyltransferase, glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base A (15/12); total (27/27) |
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGAATATCAGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGTTAGA > minE/2463247‑2463375
|
agaaTATCAGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGa > 1:2291015/1‑71 (MQ=255)
agaaTATCAGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGa > 1:1355788/1‑71 (MQ=255)
agaaTATCAGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGa > 1:1590476/1‑71 (MQ=255)
atCAGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTa > 1:3182684/1‑69 (MQ=255)
cAGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAg > 1:752748/1‑70 (MQ=255)
aGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAg > 1:3684396/1‑69 (MQ=255)
aGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCa < 1:2216369/71‑1 (MQ=255)
gTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCaaa > 1:2286110/1‑71 (MQ=255)
gAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATaa < 1:3122614/71‑1 (MQ=255)
gAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATa > 1:2723304/1‑70 (MQ=255)
aGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAaatt < 1:2057329/70‑1 (MQ=255)
tGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAaatttaa < 1:354448/71‑1 (MQ=255)
ggTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAaatttaat < 1:3334682/71‑1 (MQ=255)
ggTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAaatttaat > 1:2707410/1‑71 (MQ=255)
gTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAaatttaatt > 1:2257184/1‑71 (MQ=255)
tAAACTTCAGGTTCCAGCCCTGTATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAAtttt > 1:990765/1‑71 (MQ=255)
aaCTTCATGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAAttttt < 1:1149642/70‑1 (MQ=255)
aaCTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTa > 1:2663096/1‑71 (MQ=255)
aCTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTAt < 1:182501/71‑1 (MQ=255)
cTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTAt > 1:1518887/1‑70 (MQ=255)
cAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATaaaa > 1:625166/1‑55 (MQ=255)
cAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATaaaa > 1:629597/1‑55 (MQ=255)
gTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGAt < 1:2996946/38‑1 (MQ=255)
gTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGAt < 1:3660721/38‑1 (MQ=255)
gTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGAt < 1:343814/38‑1 (MQ=255)
gTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCaaaaaa < 1:696867/71‑1 (MQ=255)
gTTCCAGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATGTAATTTTTATCaaaaaa < 1:1082981/71‑1 (MQ=255)
ccAGCCCTGGATGGCGAACGTCGTCTGGTGGTGATTAAAGCAAATAAaatttaattt < 1:2868339/57‑1 (MQ=255)
ccAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAaatttaattt < 1:1904017/57‑1 (MQ=255)
aGCCCTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATaaa < 1:3002112/46‑1 (MQ=255)
gCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAaatt < 1:1010504/48‑1 (MQ=255)
gCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATa < 1:3126092/71‑1 (MQ=255)
gCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATa < 1:3180047/71‑1 (MQ=255)
gCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATa < 1:2050815/71‑1 (MQ=255)
cccTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATa > 1:785631/1‑70 (MQ=255)
cccTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATaa < 1:1940751/71‑1 (MQ=255)
ccTGGATCGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATaa > 1:2151881/1‑70 (MQ=255)
cTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAAttttt > 1:712962/1‑53 (MQ=255)
cTGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATaaa > 1:479486/1‑70 (MQ=255)
tGGATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAaatttaa < 1:3403224/47‑1 (MQ=255)
gATGTCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAAt < 1:3644742/71‑1 (MQ=255)
gATGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATaaaaa < 1:3251084/69‑1 (MQ=255)
gATGGCGAACGTCATCTGGGGGTGATTAAAGCAAATaaa < 1:2359335/39‑1 (MQ=255)
aTGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAAT‑ATAAAAAATTg < 1:2973260/71‑1 (MQ=255)
aTGGCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAAt > 1:894128/1‑60 (MQ=255)
ggCGAACGTCATCTGGTGGTGATTAAAGCAAATAAaat > 1:3522137/1‑38 (MQ=255)
ggCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGa > 1:1224733/1‑71 (MQ=255)
ggCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGa > 1:561441/1‑71 (MQ=255)
ggCGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGa > 1:3370109/1‑71 (MQ=255)
cGAACGTCATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGAcc < 1:2333071/71‑1 (MQ=255)
gAACGTCATCTGGTGGTGATTAAAGCAAATAAaattt < 1:2859631/37‑1 (MQ=255)
aCGTCATCTGGTGGTGATTAAAGCAAATAAaatttaa > 1:1065317/1‑37 (MQ=255)
aCGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGt > 1:1077749/1‑71 (MQ=255)
cATCTGGTAGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGTTAGa > 1:3048369/1‑71 (MQ=255)
|
AGAATATCAGGTCGAATCAGTGGTTAAACTTCAGGTTCCAGCCCTGGATGGCGAACGTCATCTGGTGGTGATTAAAGCAAATAAAATTTAATTTTTATCAAAAAAATCATAAAAAATTGACCGGTTAGA > minE/2463247‑2463375
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
