Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F8 I0 R2
|
53 |
71.6 |
5374071 |
98.5% |
5293459 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,249,944 |
C→A |
9.9% |
P89T (CCG→ACG) |
yqjG → |
predicted S‑transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,249,944 | 0 | C | A | 9.9%
| 224.0
/ 6.7
| 70 | P89T (CCG→ACG) | yqjG | predicted S‑transferase |
| Reads supporting (aligned to +/- strand): ref base C (30/33); new base A (3/4); total (33/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01 |
CGCACCGCACGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGCTCTA > W3110S.gb/3249876‑3250011
|
cgcaccgcacGCTGATCATGCGCAAACTCAAAGGACTGGAACCTTTTATTTCCGTTTCCGTAGTGAAccc < 1:5148837/70‑1 (MQ=255)
gcaccgcacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGc < 1:4766987/71‑1 (MQ=255)
caccgcacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCt > 1:730997/1‑71 (MQ=255)
caccgcacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCt < 1:3632376/71‑1 (MQ=255)
ccgcacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGc > 1:5114746/1‑68 (MQ=255)
ccgcacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCt < 1:728357/69‑1 (MQ=255)
gcacTCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATg < 1:2214512/71‑1 (MQ=255)
gcacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATg > 1:2230789/1‑71 (MQ=255)
cacGCTGATCATGCTCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGc < 1:2194682/71‑1 (MQ=255)
cacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGc > 1:645208/1‑71 (MQ=255)
cacGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGc > 1:3531655/1‑71 (MQ=255)
aTGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACgg < 1:1577801/71‑1 (MQ=255)
aTGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACgg < 1:1553367/71‑1 (MQ=255)
aTGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACgg < 1:2733541/71‑1 (MQ=255)
aTGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACg < 1:1505165/70‑1 (MQ=255)
cgcAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTg < 1:2645548/71‑1 (MQ=255)
cgcAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTg < 1:1132116/71‑1 (MQ=255)
cAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACACGCTGATGCTGGaaaa > 1:4825644/1‑63 (MQ=255)
aCTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAAccc < 1:3256975/45‑1 (MQ=255)
aCTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTg > 1:3305938/1‑66 (MQ=255)
cAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGa > 1:1138120/1‑65 (MQ=255)
cAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCttt > 1:4998757/1‑70 (MQ=255)
cAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACACGCTGATGCTGGAAAACGGTTGGAcc < 1:1290338/67‑1 (MQ=255)
aaaGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCttt < 1:3676076/69‑1 (MQ=255)
aaaGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTtga > 1:19343/1‑71 (MQ=255)
aaaGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTtg < 1:1450103/70‑1 (MQ=255)
aaGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTtgat < 1:2026842/71‑1 (MQ=255)
aaGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTtgat > 1:2527548/1‑71 (MQ=255)
aGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATg > 1:2512200/1‑46 (MQ=255)
aGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACgg < 1:3060217/57‑1 (MQ=255)
gACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGaa < 1:634139/50‑1 (MQ=255)
gACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTtgatga > 1:2946178/1‑70 (MQ=255)
aCTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTTGAAAACGGTTGGACCTTTGATGACa < 1:3501452/71‑1 (MQ=255)
cTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAg > 1:3124445/1‑71 (MQ=255)
gAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGttt > 1:5073628/1‑71 (MQ=255)
gAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGttt < 1:1744831/71‑1 (MQ=255)
aCCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTc > 1:790501/1‑71 (MQ=255)
ccGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCttt < 1:3468327/57‑1 (MQ=255)
ccGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTcc < 1:3288837/71‑1 (MQ=255)
gTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg < 1:1094442/71‑1 (MQ=255)
gTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg < 1:2998027/71‑1 (MQ=255)
gTTTATTTCCGTTTCCGTAGTGAACACGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg < 1:3897780/71‑1 (MQ=255)
gTTTATTTCCGTTTCAGTAGTGAACACGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg < 1:4760233/71‑1 (MQ=255)
tttATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCggg < 1:1570003/71‑1 (MQ=255)
ttATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg > 1:5296767/1‑69 (MQ=255)
tATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGaaaa > 1:3723995/1‑39 (MQ=255)
tATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGa < 1:4035196/70‑1 (MQ=255)
tATTTCCGTTTCCGTAGTGAACACGCTGATGCTGGaaaa > 1:2032085/1‑39 (MQ=255)
tttccgtttccgTAGTGAACCCGCTGATGCTGGAAAAc > 1:4056306/1‑38 (MQ=255)
tgcgtttccgtAGTGAACACGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGa > 1:1407495/3‑66 (MQ=255)
cgtttccgtAGTGAACCGGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAAcc < 1:4261569/70‑1 (MQ=255)
cgtttccgtAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCg < 1:1677239/71‑1 (MQ=255)
ttccgtAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCg > 1:1846811/1‑58 (MQ=255)
tccgtAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg > 1:2382326/1‑58 (MQ=255)
ccgtAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAg > 1:1251087/1‑60 (MQ=255)
cgtAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGac < 1:3662168/70‑1 (MQ=255)
aGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCg > 1:1695041/1‑52 (MQ=255)
aGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCg < 1:5007773/52‑1 (MQ=255)
aGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGc > 1:3270550/1‑71 (MQ=255)
aGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGc > 1:187630/1‑71 (MQ=255)
gTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGct > 1:4136144/1‑71 (MQ=255)
tGAACCCGCTGATGCTGGAAAACGGTTGGACCttt < 1:3755496/35‑1 (MQ=255)
tGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAg < 1:3939133/43‑1 (MQ=255)
tGAACCCGATGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCaa < 1:613215/57‑1 (MQ=255)
gAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg > 1:35241/1‑50 (MQ=255)
gAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCgg > 1:3516083/1‑50 (MQ=255)
aaCCCGCTGATGCTGGAAAACGGTTGGACCTTtga > 1:186008/1‑35 (MQ=255)
aaCCCGCTGATGCTGGAAAACGGTTGGACCTTtga > 1:931889/1‑35 (MQ=255)
aaCCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAAcc < 1:40959/57‑1 (MQ=255)
aaCCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGCTCTa > 1:4425536/1‑71 (MQ=255)
aaCCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGCTCTa < 1:939807/71‑1 (MQ=255)
aaCACGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGCTCTa < 1:5181491/71‑1 (MQ=255)
|
CGCACCGCACGCTGATCATGCGCAAACTCAAAGGACTGGAACCGTTTATTTCCGTTTCCGTAGTGAACCCGCTGATGCTGGAAAACGGTTGGACCTTTGATGACAGTTTTCCGGGAGCAACCGGAGACACGCTCTA > W3110S.gb/3249876‑3250011
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
