Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
311,469 |
A→G |
20.9% |
R468R (CGT→CGC) |
acrB ← |
multidrug efflux system protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 311,469 | 0 | A | G | 20.9%
| 304.6
/ 61.8
| 129 | R468R (CGT→CGC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (44/58); new base G (9/18); total (53/76) |
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 |
Only 100 of 142 total aligned reads displayed.
GAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGA > minE/311399‑311539
|
gagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGa < 1:4798511/71‑1 (MQ=255)
gagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGa > 1:1974332/1‑71 (MQ=255)
gagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGa < 1:5594981/71‑1 (MQ=255)
gCTGGAGTCAGGATCAACGCCACCAGTACCTACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:303446/70‑1 (MQ=255)
gCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:4281210/70‑1 (MQ=255)
ggAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga > 1:4453816/1‑69 (MQ=255)
gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:1889252/70‑1 (MQ=255)
aGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga > 1:1595649/1‑67 (MQ=255)
tCAGGATCAACGCCACCAGTACAGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatagata < 1:4491167/71‑1 (MQ=255)
agaaTCAATGCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGc < 1:4657457/68‑1 (MQ=9)
aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:3808007/61‑1 (MQ=255)
gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCa > 1:5493319/1‑70 (MQ=255)
gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:4386025/1‑71 (MQ=255)
gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:5080207/1‑71 (MQ=255)
aTCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:1375858/58‑1 (MQ=255)
tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:1338692/1‑63 (MQ=255)
tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:2619024/1‑63 (MQ=255)
tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:3183534/1‑69 (MQ=255)
tCAACGCCACCAGTACCGACAGCGCCATTGCTCAAACAATGGTAATAGAGAACTGAc < 1:1154881/57‑1 (MQ=255)
cAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTATTAGAGAACTGACGATAGATAGCACCAg > 1:3482320/1‑71 (MQ=255)
aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg < 1:483869/70‑1 (MQ=255)
cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:5619597/71‑1 (MQ=255)
cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:5271663/71‑1 (MQ=255)
gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGa < 1:228216/71‑1 (MQ=255)
ccaccaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgataaat < 1:1538083/58‑1 (MQ=9)
ccaccaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgataaat < 1:5341182/58‑1 (MQ=9)
ccaccaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgataaat < 1:4087024/58‑1 (MQ=9)
caccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa < 1:1981799/70‑1 (MQ=255)
caccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa < 1:3828326/70‑1 (MQ=255)
gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:93049/71‑1 (MQ=255)
gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:1905006/71‑1 (MQ=255)
tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:3704843/1‑50 (MQ=255)
ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:1057858/60‑1 (MQ=255)
cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatag < 1:3576800/46‑1 (MQ=255)
cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatag < 1:2221125/46‑1 (MQ=255)
cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:2455357/71‑1 (MQ=255)
cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:1989027/71‑1 (MQ=255)
gaaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCTATAAATTGCCCCAGTAGAACCGCCgaagaa < 1:3210604/68‑1 (MQ=12)
gaaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaa < 1:2556783/68‑1 (MQ=12)
gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag > 1:1456554/1‑69 (MQ=255)
aaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgata < 1:4078824/41‑1 (MQ=14)
aaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaat > 1:4834798/3‑70 (MQ=12)
aCAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaag > 1:3356927/1‑71 (MQ=255)
cAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAgg > 1:96034/1‑71 (MQ=255)
aGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGc > 1:2207104/1‑71 (MQ=12)
aGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGc > 1:454850/1‑71 (MQ=12)
cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:4182131/1‑66 (MQ=255)
cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:9675/1‑66 (MQ=255)
cgcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGccc > 1:1488991/1‑45 (MQ=2)
cgcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGccc > 1:5450556/1‑45 (MQ=2)
cgcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGcca > 1:4314905/1‑45 (MQ=2)
cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCa > 1:2871521/1‑46 (MQ=255)
cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCa > 1:2991884/1‑46 (MQ=255)
cATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGccg < 1:1130810/58‑2 (MQ=12)
aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:5433301/71‑1 (MQ=255)
ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:3313267/58‑1 (MQ=255)
ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:1600655/1‑70 (MQ=255)
ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:64888/71‑1 (MQ=255)
ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:5332686/71‑1 (MQ=255)
ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:3403910/71‑1 (MQ=255)
tGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCgga > 1:1026478/1‑69 (MQ=9)
gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:5141432/46‑1 (MQ=255)
gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:260148/46‑1 (MQ=255)
gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:2425457/1‑71 (MQ=255)
gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:5294934/1‑71 (MQ=255)
gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:1691408/1‑71 (MQ=255)
cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc < 1:4707146/70‑1 (MQ=255)
cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:4232480/71‑1 (MQ=255)
gAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:4973337/1‑71 (MQ=255)
aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa < 1:2727620/55‑1 (MQ=255)
aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:4185394/68‑1 (MQ=255)
aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:744121/68‑1 (MQ=255)
aCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:2289126/1‑62 (MQ=255)
cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc < 1:45062/71‑1 (MQ=255)
aTGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc > 1:4106045/1‑71 (MQ=255)
tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:4585766/71‑2 (MQ=9)
tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct > 1:4012000/1‑70 (MQ=9)
tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:4220291/71‑2 (MQ=9)
gTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa < 1:2636719/71‑1 (MQ=255)
aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:3368717/1‑59 (MQ=255)
aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg > 1:4973468/1‑71 (MQ=255)
tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa < 1:679377/69‑1 (MQ=255)
agagAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa < 1:788602/66‑1 (MQ=255)
agagAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTa > 1:1350901/1‑71 (MQ=255)
gagaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:5282410/1‑53 (MQ=255)
agaACTGACGATAGATAGCACCACTAGAACCCCCAaag < 1:2297252/38‑1 (MQ=38)
gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:2545083/71‑1 (MQ=255)
gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:1460261/71‑1 (MQ=255)
gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:3284158/71‑1 (MQ=255)
aCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:2281518/70‑1 (MQ=9)
aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa < 1:1229471/48‑1 (MQ=255)
cTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg > 1:3512703/1‑71 (MQ=255)
gACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt > 1:753224/1‑40 (MQ=255)
aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:4104221/63‑1 (MQ=255)
aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:2462337/63‑1 (MQ=255)
aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgcg < 1:61448/70‑1 (MQ=255)
aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgcg < 1:372901/70‑1 (MQ=255)
aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:2999704/1‑71 (MQ=255)
aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:3048474/1‑71 (MQ=255)
aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:3423818/1‑71 (MQ=255)
|
GAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGA > minE/311399‑311539
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
