Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
327,386 |
C→T |
79.6% |
S288F (TCC→TTC) |
hemH → |
ferrochelatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 327,386 | 0 | C | T | 79.6%
| 82.5
/ 16.8
| 44 | S288F (TCC→TTC) | hemH | ferrochelatase |
| Reads supporting (aligned to +/- strand): ref base C (3/6); new base T (16/19); total (19/25) |
| Fisher's exact test for biased strand distribution p-value = 7.10e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 |
ATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACA > minE/327316‑327446
|
aTGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGtctc > 1:5462294/1‑71 (MQ=255)
gtgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTtcc > 1:528303/1‑71 (MQ=255)
tgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTtcc < 1:2532612/70‑1 (MQ=255)
cccGGGCTTTGCTGCGGATTGTCTGGAGACTCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTc < 1:2423086/70‑1 (MQ=255)
cccGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTc < 1:4409257/70‑1 (MQ=255)
cccGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTCCCTCg < 1:2382427/71‑1 (MQ=255)
cGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGt < 1:3520252/71‑1 (MQ=255)
gggCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTg > 1:4825803/1‑71 (MQ=255)
gggCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTg > 1:4147269/1‑71 (MQ=255)
ggCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGc > 1:5683682/1‑71 (MQ=255)
cTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGTTGCcg > 1:4257975/1‑71 (MQ=255)
cTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCcg > 1:3384408/1‑71 (MQ=255)
cTTTGCTGCGGATTGTCAGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCcg > 1:3575547/1‑71 (MQ=255)
gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTtcc < 1:4003843/58‑1 (MQ=255)
gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTCCCTCGGTGCcggc < 1:2545852/69‑1 (MQ=255)
cGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaa > 1:4227050/1‑70 (MQ=255)
ggATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaa < 1:4589376/71‑1 (MQ=255)
aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaaaa < 1:5679351/71‑1 (MQ=255)
aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaaaa < 1:5351496/71‑1 (MQ=255)
aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaaaa < 1:2653669/71‑1 (MQ=255)
tGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAata > 1:5513818/1‑71 (MQ=255)
gTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCgg > 1:5644045/1‑52 (MQ=255)
gTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCgg > 1:3656980/1‑52 (MQ=255)
tCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaa < 1:4862967/65‑1 (MQ=255)
tCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaa < 1:4107293/65‑1 (MQ=255)
tCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATg < 1:3866350/71‑1 (MQ=255)
gtgaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAata < 1:1136603/69‑1 (MQ=255)
gagaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAata < 1:4522512/71‑1 (MQ=255)
gagaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAata < 1:5629541/71‑1 (MQ=255)
agaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAata > 1:2099505/1‑70 (MQ=255)
gCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaaaa < 1:4172247/58‑1 (MQ=255)
gCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaaaa < 1:415940/58‑1 (MQ=255)
gCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGaa > 1:3753518/1‑64 (MQ=255)
agagATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGgcgc > 1:2749230/1‑71 (MQ=255)
gCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAatata > 1:5017076/1‑55 (MQ=255)
cGAGCAAAACCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAatata < 1:3800887/53‑1 (MQ=255)
cGAGCAAAACCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAatata < 1:4206465/53‑1 (MQ=255)
gCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACg > 1:2931168/1‑70 (MQ=255)
aaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCgg < 1:5658351/71‑1 (MQ=255)
aaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAat < 1:2176468/43‑1 (MQ=255)
aaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAat < 1:2407127/43‑1 (MQ=255)
aaCCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGa < 1:5137334/71‑1 (MQ=255)
aCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGcc > 1:5411836/1‑67 (MQ=255)
aCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGaa > 1:4138786/1‑71 (MQ=255)
aCCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGc > 1:4097089/1‑61 (MQ=255)
aCCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGc > 1:1788376/1‑61 (MQ=255)
cGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACa < 1:527292/71‑1 (MQ=255)
gTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGa < 1:3981405/62‑1 (MQ=255)
tctcCCTCGGTGCCGGCGGGAAAAAATATGAatata < 1:4938660/36‑1 (MQ=255)
|
ATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTCCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACA > minE/327316‑327446
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
