Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
385,238 |
C→A |
32.2% |
L150M (CTG→ATG) |
cstA → |
carbon starvation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 385,238 | 0 | C | A | 32.2%
| 95.6
/ 41.9
| 62 | L150M (CTG→ATG) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base C (22/20); new base A (10/10); total (32/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 |
GCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCA > minE/385169‑385307
|
gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGc < 1:2879691/70‑1 (MQ=255)
gCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCt > 1:2012251/1‑71 (MQ=255)
ccGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTg < 1:5132589/71‑1 (MQ=255)
ggtgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGt > 1:3763399/1‑71 (MQ=255)
gtgcggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTc < 1:1909713/71‑1 (MQ=255)
ggtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaag < 1:1055205/71‑1 (MQ=255)
gtgcAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGCTCGC‑TCGCTGGGTGAGCTGGTCAaaga < 1:1892611/71‑1 (MQ=255)
ggACTTTATGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAagag < 1:1528555/70‑1 (MQ=255)
aTTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATg > 1:3716572/1‑71 (MQ=255)
tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg > 1:3351490/1‑71 (MQ=255)
tttCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgg < 1:3741813/71‑1 (MQ=255)
tCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaagaa > 1:1069574/1‑62 (MQ=255)
tCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaagaa > 1:741553/1‑62 (MQ=255)
tCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaagaa > 1:4164555/1‑62 (MQ=255)
tCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgggg > 1:1642879/1‑71 (MQ=255)
tCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgggg > 1:2193180/1‑71 (MQ=255)
taTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAagag > 1:4429809/2‑64 (MQ=255)
aTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac < 1:4817021/70‑3 (MQ=255)
aTGGTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATgggac < 1:5493565/70‑3 (MQ=255)
gTGCTGTTTATCTCCTCTCGCCGTAATGG‑CGCATCTCTTGGTGAGATGATCAAAGAAGAGATGGGACCag < 1:5512658/70‑2 (MQ=255)
cTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCaaa < 1:2023765/51‑1 (MQ=255)
gtttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaaga > 1:3286166/1‑51 (MQ=255)
tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCaaa < 1:2671661/48‑1 (MQ=255)
tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCaaa < 1:4307058/48‑1 (MQ=255)
tttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccgccg < 1:4726214/70‑1 (MQ=255)
ttgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCaaa > 1:3585794/1‑47 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCaa > 1:3947781/1‑45 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCaa > 1:1913823/1‑45 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAgaga > 1:5420126/1‑53 (MQ=255)
tgtttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCgggg > 1:4774703/1‑71 (MQ=255)
tttCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAagaaga < 1:2877875/49‑1 (MQ=255)
tCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAaagaa > 1:390412/1‑45 (MQ=255)
cTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAAccg < 1:37237/60‑1 (MQ=255)
gcgcCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGcc < 1:122803/51‑1 (MQ=255)
ctcgccgtaatggcgcatctcttGGTGAGATGATCAaag > 1:1745845‑M1/24‑39 (MQ=255)
ctcgccgtaatggcgcatctcttGGTGAGATGATCAaag > 1:3188056‑M1/24‑39 (MQ=255)
ctcgccgtaatggcgcatctcttGGTGAGATGATCAaag > 1:2846759‑M1/24‑39 (MQ=255)
ctcgccgtaatggcgcatctcttGGTGAGATGATCAaag > 1:5621722‑M1/24‑39 (MQ=255)
ctcgccgtaatggcgcatctcttGGTGAGATGATCAAagaaga > 1:3695404‑M1/24‑43 (MQ=255)
ctcgccgtaatggcgcatctcttGGTGAGATGATCAAagaaga > 1:3106716‑M1/24‑43 (MQ=255)
tcgccgtaatggcgcatctcttGGTGAGATGATCAAAGAagag < 1:4256615‑M1/21‑1 (MQ=255)
gccgtaatggcgcatctcttGGTGAGATGATCAaagaa < 1:475511‑M1/18‑1 (MQ=255)
gccgtaatggcgcatctcttGGTGAGATGATCAAAGAAGAGATGGGACcag < 1:857612‑M1/31‑2 (MQ=255)
ccGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCtgg < 1:5085332/71‑1 (MQ=255)
taatggcgcatctcttGGTGAGATGATCAAagaaga > 1:3596421‑M1/17‑36 (MQ=255)
gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGc > 1:968373/1‑71 (MQ=255)
gACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGc > 1:2406027/1‑71 (MQ=255)
aCGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATgggg > 1:351411/1‑41 (MQ=255)
atggcgcatctcttGGTGAGATGATCAAAGAAGAGATGGGACcag < 1:4448854‑M1/31‑2 (MQ=255)
atggcgcatctcttGGTGAGATGATCAAAGAAGAGATGGGACcag < 1:4267046‑M1/31‑2 (MQ=255)
atggcgcatctcttGGTGAGATGATCAAAGAAGAGATGGGACcag < 1:511134‑M1/31‑2 (MQ=255)
atggcgcatctcttGGTGAGATGATCAAAGAAGAGATGGGACCa > 1:2087755‑M1/15‑44 (MQ=255)
tggcgcatctcttGGTGAGATGATCAAAGAAgaga > 1:4054219‑M1/14‑35 (MQ=255)
gctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGa > 1:4633605/1‑71 (MQ=255)
ctGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGAt < 1:1695858/71‑1 (MQ=255)
tGGGTGAGCTGGTCAAAGAAGAGATTGGGCCAACCGCCGGGGTGATTg < 1:3204449/48‑1 (MQ=255)
tGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTg < 1:101812/48‑1 (MQ=255)
gggTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCtt > 1:1233697/1‑63 (MQ=255)
gAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt < 1:5362216/38‑1 (MQ=255)
gAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGt < 1:443114/38‑1 (MQ=255)
gAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGt > 1:2884073/1‑71 (MQ=255)
aGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGt < 1:5165371/70‑1 (MQ=255)
gCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCa < 1:634859/71‑1 (MQ=255)
|
GCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGC‑TCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCA > minE/385169‑385307
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
