Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R1	2892	75.3	5698161	66.1%	3766484	59.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	385,837	C→G	29.7%	T349T (ACC→ACG)	cstA →	carbon starvation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	385,837	0	C	G	29.7%	130.7 / 76.7	101	T349T (ACC→ACG)	cstA	carbon starvation protein
Reads supporting (aligned to +/- strand): ref base C (34/37); new base G (17/13); total (51/50)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01

Only 100 of 103 total aligned reads displayed.

CCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCC  >  minE/385769‑385906

ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc                                                                     <  1:5355415/71‑1 (MQ=255)

  gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                                   >  1:201029/1‑71 (MQ=255)

  gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                                   >  1:5588902/1‑71 (MQ=255)

  gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                                                                   >  1:4703036/1‑71 (MQ=255)

   ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc                                                                  <  1:168692/71‑1 (MQ=255)

   ttCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGcc                                                                  <  1:3677951/71‑1 (MQ=255)

      cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                                                                <  1:2874781/70‑3 (MQ=255)

      cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                                                                <  1:345194/70‑3 (MQ=255)

         tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             <  1:1576820/70‑1 (MQ=255)

         tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             <  1:1661703/70‑1 (MQ=255)

         tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             <  1:5116961/70‑1 (MQ=255)

               aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg                                                       >  1:3700686/1‑70 (MQ=255)

               aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc                                                      >  1:4549970/1‑71 (MQ=255)

               aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc                                                      >  1:5469831/1‑71 (MQ=255)

               aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc                                                      >  1:2658140/1‑71 (MQ=255)

                ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct                                                     <  1:3375521/71‑2 (MQ=255)

                   tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg                                                       <  1:4625930/66‑1 (MQ=255)

                   tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac                                                  >  1:5309445/1‑71 (MQ=255)

                   tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac                                                  >  1:1952477/1‑71 (MQ=255)

                    cGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat                                                  >  1:1707696/1‑69 (MQ=255)

                     gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg                                                 <  1:5170441/70‑3 (MQ=255)

                     gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg                                                 >  1:5027682/1‑68 (MQ=255)

                     gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:4227084/70‑1 (MQ=255)

                     gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:3113432/70‑1 (MQ=255)

                     gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:2238044/70‑1 (MQ=255)

                     gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:2474919/70‑1 (MQ=255)

                     gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:133727/70‑1 (MQ=255)

                     gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg                                                     >  1:3418768/1‑66 (MQ=255)

                      ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga                                                >  1:1528704/1‑70 (MQ=255)

                      ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga                                                >  1:4632422/1‑70 (MQ=255)

                       cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg                                              >  1:2125439/1‑71 (MQ=255)

                          tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc                                            <  1:89464/70‑4 (MQ=255)

                          tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct                                                     <  1:1943278/61‑2 (MQ=255)

                          tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             >  1:5612503/1‑53 (MQ=255)

                          tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             >  1:2359027/1‑53 (MQ=255)

                          tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             >  1:2217885/1‑53 (MQ=255)

                          tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             >  1:3948685/1‑53 (MQ=255)

                          tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                                                             >  1:4199975/1‑53 (MQ=255)

                           ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                           >  1:2834309/1‑54 (MQ=255)

                           ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                           >  1:4360710/1‑54 (MQ=255)

                           ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATg                                                           >  1:5336243/1‑54 (MQ=255)

                           ggCGCGGTGTCTGGCTTCCATGCGCTGATCTCTT‑AGGGACCACGCCGAAGATg                                                           >  1:2771463/1‑53 (MQ=255)

                            gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                   >  1:36651/3‑61 (MQ=255)

                            gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                   >  1:3497235/3‑61 (MQ=255)

                            gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                   >  1:5412112/3‑61 (MQ=255)

                            gtgcgGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc                                                      >  1:3204708/3‑58 (MQ=255)

                                  tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:4439594/57‑1 (MQ=255)

                                  tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:2946652/57‑1 (MQ=255)

                                  tgtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGc                                   <  1:510735/71‑1 (MQ=255)

                                    tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:1964989/55‑1 (MQ=255)

                                    tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                                 >  1:4033926/1‑71 (MQ=255)

                                    tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                                 <  1:3530569/71‑1 (MQ=255)

                                    tCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaat                                                  <  1:256970/54‑2 (MQ=255)

                                      tGGCTTCCCTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa                               >  1:4829790/1‑71 (MQ=255)

                                      tGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTa                               >  1:383174/1‑71 (MQ=255)

                                      tGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaac                                             >  1:3451681/1‑55 (MQ=255)

                                        tcTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGATTTATc                             >  1:1509315/2‑71 (MQ=255)

                                        gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt                                <  1:2768365/68‑1 (MQ=255)

                                        gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCttt                                <  1:4386772/68‑1 (MQ=255)

                                        gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATc                             >  1:889385/1‑71 (MQ=255)

                                        gCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaa                                                   >  1:3400887/1‑49 (MQ=255)

                                            ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg                                                 <  1:4855209/47‑1 (MQ=255)

                                             cATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTa                        <  1:385124/71‑1 (MQ=255)

                                             cATGCGCTGATCTCTTCTGGGACCACGCAGAAGATGCTGGCGAACGAAGGGCAGGc                                       >  1:4279267/1‑56 (MQ=255)

                                              acgcgcTGATCTCTTCTGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                       <  1:5073468/69‑1 (MQ=255)

                                              acgcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                       <  1:3511640/69‑1 (MQ=255)

                                              acgcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTAc                       <  1:3148854/69‑1 (MQ=255)

                                              aTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc                       <  1:5662606/71‑1 (MQ=255)

                                                gcgcTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTACgg                     >  1:1947638/1‑71 (MQ=255)

                                                 cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTAt                              >  1:1328776/1‑61 (MQ=255)

                                                 cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACgg                     >  1:516288/1‑70 (MQ=255)

                                                 cgcTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGt                    >  1:1336817/1‑71 (MQ=255)

                                                  gcTGATCTCTTCTGGGACCACGCCGAAGATGCAGGcgaacga                                                <  1:4413063/42‑1 (MQ=255)

                                                   cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                  <  1:1495428/71‑1 (MQ=255)

                                                   cTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                  <  1:4844150/71‑1 (MQ=255)

                                                   cTGATCTCTTCCGGTACGACGCCAAAACTGCTGGCTAATGAAACCGACGCGCGTTTCATCGGCTACggcgc                  <  1:4059700/71‑4 (MQ=255)

                                                    tGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                  <  1:3426164/70‑1 (MQ=255)

                                                     gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                               >  1:4415187/1‑40 (MQ=255)

                                                     gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                               >  1:4492211/1‑40 (MQ=255)

                                                     gATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacgaa                                               >  1:50421/1‑40 (MQ=255)

                                                      aTCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGAt               >  1:3010674/1‑71 (MQ=255)

                                                        ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACgg                     >  1:3112958/1‑63 (MQ=255)

                                                        ctctTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt             >  1:1357813/1‑71 (MQ=255)

                                                         tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                                 <  1:4180707/50‑1 (MQ=255)

                                                         tcttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCtt                                 <  1:1965076/50‑1 (MQ=255)

                                                          cttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt             >  1:4686747/1‑69 (MQ=255)

                                                          cttctGCGACCACGCCGAAGATGCTGGAGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGt             >  1:2410232/1‑69 (MQ=255)

                                                           ttctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTaa          >  1:25075/1‑71 (MQ=255)

                                                            tctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGtt            <  1:2364841/68‑1 (MQ=255)

                                                             ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa           <  1:4899299/68‑1 (MQ=255)

                                                             ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa           <  1:2760664/68‑1 (MQ=255)

                                                             ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa           <  1:2013463/68‑1 (MQ=255)

                                                             ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa           <  1:3034470/68‑1 (MQ=255)

                                                             ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTa           <  1:4011975/68‑1 (MQ=255)

                                                             ctGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAAt         >  1:1746415/1‑70 (MQ=255)

                                                                ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTgg                  <  1:761870/58‑1 (MQ=255)

                                                                ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg       <  1:5649198/69‑1 (MQ=255)

                                                                ggACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATgg       <  1:2247715/69‑1 (MQ=255)

                                                                 gACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAAt    <  1:433986/71‑1 (MQ=255)

                                                                   ccACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATcc  <  1:4484983/71‑1 (MQ=255)

CCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCC  >  minE/385769‑385906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A