Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 385,938 G→C 21.6% C383S (TGT→TCT) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE385,9380GC21.6% 190.4 / 49.4 97C383S (TGT→TCT) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (40/36);  new base C (14/7);  total (54/43)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 101 total aligned reads displayed.

GCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACG  >  minE/385868‑386005
                                                                      |                                                                   
gCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                                                                     <  1:5210306/71‑1 (MQ=255)
 agTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                                                                    <  1:171864/70‑1 (MQ=255)
   tGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                                                                  >  1:5383460/1‑71 (MQ=255)
     cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                                                                >  1:796552/1‑71 (MQ=255)
     cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                                                                <  1:2988911/71‑1 (MQ=255)
     cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                                                                 <  1:2660878/70‑1 (MQ=255)
     cTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                                                                 <  1:382975/70‑1 (MQ=255)
       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                              >  1:1024988/3‑71 (MQ=255)
       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                              >  1:2478888/3‑71 (MQ=255)
       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                              >  1:4048213/3‑71 (MQ=255)
       tcaTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                              >  1:5459176/3‑71 (MQ=255)
       tcaTCGGCTACGGAGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                              >  1:1412700/3‑71 (MQ=255)
        caTCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCatcatc                                                              >  1:2165164/2‑70 (MQ=255)
         aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCg                                                             <  1:2290757/70‑1 (MQ=255)
         aTCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCg                                                             <  1:2067750/70‑1 (MQ=255)
          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                                                                    >  1:3036305/1‑62 (MQ=255)
          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGt                                                                    >  1:3774717/1‑62 (MQ=255)
          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatca                                                                <  1:43401/66‑1 (MQ=255)
          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                            <  1:564175/70‑1 (MQ=255)
          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                            <  1:5618758/70‑1 (MQ=255)
          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                            <  1:342877/70‑1 (MQ=255)
          tCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                            <  1:3811882/70‑1 (MQ=255)
          tCGGCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaa                                                           >  1:4965950/1‑70 (MQ=255)
            ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                                                              >  1:3924332/1‑66 (MQ=255)
            ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                                                              >  1:1404056/1‑66 (MQ=255)
            ggCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                                                              >  1:4375191/1‑66 (MQ=255)
            ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGaac                                                          <  1:401342/70‑3 (MQ=255)
            ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                                                         <  1:1386348/71‑1 (MQ=255)
            ggCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAAcc                                                         >  1:3009516/1‑71 (MQ=255)
             gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg                                                        <  1:1752776/71‑1 (MQ=255)
             gCTACGGCGCAATGCTGATGGAGTCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCg                                                        <  1:396407/71‑1 (MQ=255)
                 cGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCggg                                                      <  1:1775997/69‑1 (MQ=255)
                    tGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                                                                     <  1:987350/51‑1 (MQ=255)
                     gggATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTa                                                >  1:4061144/1‑71 (MQ=255)
                       gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatcatc                                                              <  1:2527693/55‑1 (MQ=255)
                       gATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAtt                                              <  1:3964663/71‑1 (MQ=255)
                        aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATGGATCCGGGCGTGTAttt                                             >  1:319888/1‑71 (MQ=255)
                        aTGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt                                             >  1:1417581/1‑71 (MQ=255)
                          gTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTg                                           >  1:1918695/1‑71 (MQ=255)
                           ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                            <  1:782660/53‑1 (MQ=255)
                           ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                            <  1:2767376/53‑1 (MQ=255)
                           ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGa                                                            <  1:229017/53‑1 (MQ=255)
                           ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                           >  1:3080136/1‑54 (MQ=255)
                           ttAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc                                          >  1:3382033/1‑71 (MQ=255)
                            tAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                                                                 >  1:1563734/1‑47 (MQ=255)
                            tAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTatc                                                                 >  1:536425/1‑47 (MQ=255)
                             aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc                                                     >  1:5671578/1‑58 (MQ=255)
                             aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGc                                                     >  1:5313509/1‑58 (MQ=255)
                             aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt                                             >  1:957108/1‑66 (MQ=255)
                             aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGc                                          >  1:3199603/1‑69 (MQ=255)
                             aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa                                        >  1:2477081/1‑71 (MQ=255)
                             aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa                                        >  1:442668/1‑71 (MQ=255)
                             aaTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa                                        >  1:4313481/1‑71 (MQ=255)
                              aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCa                                        >  1:2778469/1‑70 (MQ=255)
                              aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt                                       <  1:3215696/71‑1 (MQ=255)
                              aTGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCAt                                       <  1:4172047/71‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                                                   <  1:2489596/42‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                                                   <  1:690437/42‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                                                   <  1:5695117/42‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                                                   <  1:1225190/42‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTAttt                                             >  1:4449895/1‑64 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                                      <  1:5190215/71‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                                      <  1:5561103/71‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                                      <  1:3588898/71‑1 (MQ=255)
                               tGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                                      <  1:4695503/71‑1 (MQ=255)
                                ggAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTCTATTTTGCCATg                                      >  1:5022132/1‑70 (MQ=255)
                                 gAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                                                                     >  1:5454546/1‑38 (MQ=255)
                                 gAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGa                                     <  1:1944747/70‑1 (MQ=255)
                                   aTCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTg                                                                     <  1:696274/36‑1 (MQ=255)
                                    tCCTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACac                                 <  1:1128206/71‑2 (MQ=255)
                                     ccTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                                                   >  1:3489680/1‑36 (MQ=255)
                                      cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                                                                  >  1:4934104/1‑36 (MQ=255)
                                      cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                                                                  >  1:140140/1‑36 (MQ=255)
                                      cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTat                                                                  >  1:5364521/1‑36 (MQ=255)
                                      cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTa                                                                   >  1:2975129/1‑35 (MQ=255)
                                      cTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGcc                               >  1:144419/1‑71 (MQ=255)
                                      cTTCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc                               >  1:5577617/1‑71 (MQ=255)
                                        tCGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCg                             >  1:1968933/1‑71 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                           >  1:3225489/1‑40 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                           >  1:3701285/1‑40 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                           >  1:5564122/1‑40 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                           >  1:1710372/1‑40 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGAt                                                           >  1:2813461/1‑40 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc                              <  1:5523451/69‑1 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc                              >  1:4800594/1‑69 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc                            >  1:2396515/1‑70 (MQ=255)
                                         cGTGGCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc                            >  1:1954198/1‑70 (MQ=255)
                                         cGTGGCGATTACGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc                              <  1:1744653/69‑1 (MQ=255)
                                             gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc                            <  1:1170488/67‑2 (MQ=255)
                                             gCGATTATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACACCCCgc                            <  1:1177082/67‑2 (MQ=255)
                                                aTTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGt                     <  1:5356536/71‑1 (MQ=255)
                                                  tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc                               >  1:122627/1‑59 (MQ=255)
                                                  tATGGCGCTGGTTGCTGCGTCCATCATCGAACCGGGTCTTTACTTCGCGATGAACAccc                               >  1:2232338/1‑59 (MQ=255)
                                                    tGGCGCTGGTTTCCGCCTGTATCATCGAGCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCt                  <  1:2370925/70‑1 (MQ=255)
                                                         cTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATg                          >  1:4033427/1‑57 (MQ=255)
                                                           ggTTTCCGCCTGTATCATCGATCCTGGCGTGTATTTTGCCATGAACAGccc                              <  1:46340/51‑1 (MQ=255)
                                                           ggTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGccc                              <  1:1721911/51‑1 (MQ=255)
                                                               tCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATg                                      <  1:5483642/39‑1 (MQ=255)
                                                               tCCGCCTGTATCATCGATCCGGGCGTGTATTTAGCCATg                                      <  1:1252400/39‑1 (MQ=255)
                                                                   ccTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGAcg  >  1:3031944/1‑71 (MQ=255)
                                                                      |                                                                   
GCGTGCTTTATCGGCTACGGTGGGATGTTAATGGAATCCTTCGTGGCGATTATGGCGCTGGTTTCCGCCTGTATCATCGATCCGGGCGTGTATTTTGCCATGAACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACG  >  minE/385868‑386005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A