Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
412,090 |
A→G |
59.5% |
V89A (GTG→GCG) |
leuS ← |
leucyl‑tRNA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 412,090 | 0 | A | G | 59.5%
| 22.6
/ 40.9
| 42 | V89A (GTG→GCG) | leuS | leucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (9/8); new base G (14/11); total (23/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
TTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTG > minE/412028‑412160
|
ttttCAGATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTc > 1:737738/1‑70 (MQ=255)
ttCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCg < 1:498557/70‑1 (MQ=255)
tCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCg > 1:2713103/1‑69 (MQ=255)
atatACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCgcagg < 1:4687293/71‑1 (MQ=255)
taCGCGATGTTGTCTTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTc < 1:4714640/62‑1 (MQ=255)
cgcgATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGAc < 1:4475258/71‑1 (MQ=255)
gATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCaa < 1:187431/71‑1 (MQ=255)
gATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCa > 1:1670534/1‑70 (MQ=255)
aTGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCg < 1:1384771/58‑1 (MQ=255)
gttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAAcg > 1:3148891/1‑71 (MQ=255)
gttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAAcg > 1:340576/1‑71 (MQ=255)
ttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAcgc > 1:1194897/1‑71 (MQ=255)
ttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAcg < 1:3981078/70‑1 (MQ=255)
ttgtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAcg < 1:31688/70‑1 (MQ=255)
tgtCGTACGTCCACGGTGCCGGAGCGGTTTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAAcg < 1:3103002/69‑1 (MQ=255)
gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCgcaggca > 1:1546089/1‑59 (MQ=255)
gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCgcaggca > 1:4899165/1‑59 (MQ=255)
gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCgcaggca > 1:3249420/1‑59 (MQ=255)
gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCgcaggca > 1:3033253/1‑59 (MQ=255)
gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCgcaggca > 1:3193693/1‑59 (MQ=255)
gtCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCgcaggca > 1:1734484/1‑59 (MQ=255)
gTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCgca < 1:4082168/52‑1 (MQ=255)
gTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCgca < 1:4080263/52‑1 (MQ=255)
cGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGc > 1:3179455/1‑71 (MQ=255)
cACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGAt > 1:99393/1‑71 (MQ=255)
cACGGTGCCGGAG‑GGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc > 1:926991/1‑71 (MQ=255)
aCGGTGCCGGAGCGGTGTTGTTTTTCACCGCGGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATc > 1:505171/1‑71 (MQ=255)
cGGTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCg < 1:83354/71‑1 (MQ=255)
gTGCCGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGc > 1:4694087/1‑71 (MQ=255)
gTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGc > 1:5167527/1‑71 (MQ=255)
tGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTcc < 1:1935059/58‑1 (MQ=255)
ccGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTg < 1:2639676/70‑1 (MQ=255)
ccGGAGCGGTGTTGTTTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTg < 1:4601966/70‑1 (MQ=255)
gttTTTCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGtt < 1:5463912/66‑1 (MQ=255)
gttTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGc > 1:1351614/1‑71 (MQ=255)
gttTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGc > 1:555186/1‑71 (MQ=255)
gttTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGc > 1:2437607/1‑71 (MQ=255)
ttttCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCg < 1:1213575/47‑1 (MQ=255)
tCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAg < 1:144348/70‑1 (MQ=255)
tCACCGCCGCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAg > 1:1456907/1‑70 (MQ=255)
aCCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCa > 1:10218/1‑70 (MQ=255)
cgccgcGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATa < 1:5640122/70‑1 (MQ=255)
ccACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGc < 1:212344/71‑1 (MQ=255)
aCGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTg > 1:240601/1‑71 (MQ=255)
|
TTTTCATATACGCGATGTTGTCGTACGTCCACGGTGCCGGAGCGGTGTTGTTTTTCACCGCCACGCCTTCCGCAGGCAGACCAAACGCGTCCCAGCCGATCGGCTGCAGGACGTTTTTGCCCAGCATACGCTG > minE/412028‑412160
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
