Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I0 R1	2892	75.3	5698161	66.1%	3766484	59.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	834,992	T→G	25.2%	V1150V (GTT→GTG)	narG →	nitrate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	834,992	0	T	G	25.2%	136.0 / 73.0	103	V1150V (GTT→GTG)	narG	nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand): ref base T (41/36); new base G (18/8); total (59/44)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCT  >  minE/834924‑835061

cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                                                      >  1:4659357/1‑70 (MQ=255)

cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                                                      >  1:1986201/1‑70 (MQ=255)

cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                                                      >  1:1525425/1‑70 (MQ=255)

  gggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGt                                                                     >  1:3106948/1‑69 (MQ=255)

    gTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc                                                                 <  1:4109995/71‑1 (MQ=255)

    gTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc                                                                 <  1:2836867/71‑1 (MQ=255)

     tATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGtt                                                                       <  1:5235198/64‑1 (MQ=255)

       tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagccag                                                              >  1:3045687/1‑71 (MQ=255)

         gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTca                                                                   >  1:2057095/3‑64 (MQ=255)

         gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                                                            >  1:3982122/3‑71 (MQ=255)

         gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                                                            >  1:1991539/3‑71 (MQ=255)

         gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                                                            >  1:5386486/3‑71 (MQ=255)

         gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGGCAGCg                                                            >  1:1210870/1‑71 (MQ=255)

         gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                                                            >  1:5591984/1‑71 (MQ=255)

         gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                                                            >  1:4128563/1‑71 (MQ=255)

         gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                                                            >  1:1421242/1‑71 (MQ=255)

            gATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtg                                                          <  1:3609679/70‑1 (MQ=255)

             aTAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtgta                                                        <  1:1640830/71‑2 (MQ=255)

             aTAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGtt                                                        >  1:1871631/1‑71 (MQ=255)

              tAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtgtac                                                       >  1:25384/1‑69 (MQ=255)

              tAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtgtac                                                       >  1:1578511/1‑69 (MQ=255)

              tAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTc                                                       <  1:4348712/71‑1 (MQ=255)

               aaCGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTAcc                                                      >  1:5064802/1‑71 (MQ=255)

               aaCGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                                                      >  1:4115595/1‑71 (MQ=255)

               aaCGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                                                      >  1:4437493/1‑71 (MQ=255)

                  gACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtg                                                          <  1:2911574/64‑1 (MQ=255)

                  gACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtg                                                          <  1:4463087/64‑1 (MQ=255)

                  gACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc                                                   <  1:3507533/71‑1 (MQ=255)

                   aCTGGATTGAAGTCTTCAACAGAAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCa                                                  <  1:4126060/71‑1 (MQ=255)

                    cTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAg                                                 >  1:1206532/1‑71 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGt                                                                     >  1:5522397/1‑48 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:3777645/69‑1 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:1166388/69‑1 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:3770936/69‑1 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:4414455/69‑1 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:234117/69‑1 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:4852113/69‑1 (MQ=255)

                       gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGa                                              >  1:3396998/1‑71 (MQ=255)

                           gAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGAc                                          >  1:2012149/1‑71 (MQ=255)

                            aaGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGAc                                          <  1:388643/70‑1 (MQ=255)

                             aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                        <  1:114199/71‑1 (MQ=255)

                             aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                        <  1:5112215/71‑1 (MQ=255)

                              gtattCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCa                                        >  1:4559720/4‑70 (MQ=255)

                              gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                       >  1:4671006/1‑71 (MQ=255)

                              gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                       >  1:277424/1‑71 (MQ=255)

                              gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                       >  1:4921961/1‑71 (MQ=255)

                              gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                       >  1:1337076/1‑71 (MQ=255)

                                 ttCAACGCCAACGGCGCGCTGACTGCCCGCGCGGGGGTCAGCCAACgtgt                                                         <  1:4139325/50‑1 (MQ=12)

                                 ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:3374290/59‑1 (MQ=255)

                                 ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                <  1:828517/59‑1 (MQ=255)

                                 ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgat                                    >  1:1140239/1‑71 (MQ=255)

                                   cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGAt                                             >  1:1074713/1‑60 (MQ=255)

                                   cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGAt                                             >  1:783057/1‑60 (MQ=255)

                                      cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc                               <  1:3832943/71‑1 (MQ=255)

                                       gccAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCa                              >  1:1251324/3‑71 (MQ=255)

                                        ccAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAc                             >  1:4609626/2‑71 (MQ=255)

                                          aaCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgc                           >  1:906405/1‑71 (MQ=255)

                                          aaCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgc                           >  1:1958381/1‑71 (MQ=255)

                                          aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc                           >  1:828027/1‑71 (MQ=255)

                                           aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAg                                                 <  1:3711934/48‑1 (MQ=255)

                                           aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat                                >  1:2962100/1‑64 (MQ=255)

                                           aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat                                >  1:1890195/1‑64 (MQ=255)

                                           aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat                                >  1:2708417/1‑64 (MQ=255)

                                           aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgcc                          >  1:3788152/1‑70 (MQ=255)

                                            cGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATg                                            <  1:2474331/52‑1 (MQ=255)

                                             ggTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                          >  1:4350576/1‑69 (MQ=255)

                                              gTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAg                       <  1:1291937/71‑1 (MQ=255)

                                               tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg                      <  1:859508/71‑1 (MQ=255)

                                                 cgctGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa                    <  1:412202/69‑1 (MQ=255)

                                                  tctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc                                                   <  1:5557475/39‑1 (MQ=255)

                                                   ctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                  >  1:447976/1‑71 (MQ=255)

                                                    tGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTTCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                  >  1:4915988/1‑70 (MQ=255)

                                                     gACTGCCCGTGCGGTTGTCAGGCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa                >  1:4388736/1‑71 (MQ=255)

                                                     gACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa                >  1:2540680/1‑71 (MQ=255)

                                                      aCTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt               >  1:91440/1‑71 (MQ=255)

                                                        tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                   <  1:836604/49‑1 (MQ=255)

                                                        tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                   <  1:1829981/49‑1 (MQ=255)

                                                          cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg             <  1:661469/69‑1 (MQ=255)

                                                          cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt           <  1:4116101/71‑1 (MQ=255)

                                                          cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGt            >  1:2246713/1‑70 (MQ=255)

                                                          cccGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAttat            <  1:3019946/70‑4 (MQ=255)

                                                          cccGCGCGGTGGTCAACCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAttat            >  1:45183/1‑67 (MQ=255)

                                                           ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                 <  1:3707454/64‑1 (MQ=255)

                                                           ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                 <  1:1621651/64‑1 (MQ=255)

                                                             gTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTAt               <  1:2908133/64‑1 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                        <  1:2566865/38‑1 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                 >  1:293482/1‑45 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                 >  1:1104819/1‑45 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                 >  1:641543/1‑45 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                 >  1:1371684/1‑45 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt           <  1:2860641/67‑1 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt           <  1:679706/67‑1 (MQ=255)

                                                              tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc       >  1:2168996/1‑71 (MQ=255)

                                                               gCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcct      >  1:1216563/1‑71 (MQ=255)

                                                                cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg     >  1:4223642/1‑71 (MQ=255)

                                                                cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg     >  1:3045363/1‑71 (MQ=255)

                                                                  gTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                <  1:4478773/42‑1 (MQ=255)

                                                                   ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct  <  1:2602551/71‑1 (MQ=255)

                                                                   ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct  <  1:1044911/71‑1 (MQ=255)

                                                                   ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct  <  1:3365965/71‑1 (MQ=255)

CTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCT  >  minE/834924‑835061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A