Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
835,873 |
G→A |
33.5% |
P197P (CCG→CCA) |
narH → |
nitrate reductase 1, beta (Fe‑S) subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 835,873 | 0 | G | A | 33.5%
| 88.7
/ 66.7
| 81 | P197P (CCG→CCA) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/30); new base A (15/12); total (39/42) |
| Fisher's exact test for biased strand distribution p-value = 3.49e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGG > minE/835803‑835942
|
acacCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCg < 1:1157043/71‑1 (MQ=255)
acacCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCg < 1:3126183/71‑1 (MQ=255)
acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:1122157/71‑1 (MQ=255)
acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:2239291/71‑1 (MQ=255)
acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:3395560/71‑1 (MQ=255)
acCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:2812730/71‑1 (MQ=255)
ccTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAg < 1:5009832/70‑1 (MQ=255)
ccTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGc > 1:1982968/1‑71 (MQ=255)
tCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGt > 1:4422061/1‑71 (MQ=255)
tCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCggc > 1:1641382/1‑70 (MQ=255)
cATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCggc < 1:4020327/70‑2 (MQ=255)
cATGATGTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCgg < 1:860058/69‑1 (MQ=255)
atgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCgg < 1:73443/68‑1 (MQ=255)
atgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGgcgc > 1:3207135/1‑71 (MQ=255)
tgatgTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCg < 1:5125726/71‑1 (MQ=255)
tgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGgcgct < 1:5492776/71‑2 (MQ=255)
tgatgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCACAGC‑TGCGTGGCGACCTGCCCAAGCGgcgc > 1:3424952/1‑70 (MQ=255)
atgTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAt < 1:5454100/71‑1 (MQ=255)
atgTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGAt < 1:4593942/71‑1 (MQ=255)
atgTACCTGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGgcgcta < 1:1889141/70‑3 (MQ=255)
cctGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTAc > 1:2959457/3‑70 (MQ=255)
cctGCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTAc > 1:965612/3‑70 (MQ=255)
tGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACATGCCCGAGCGGTGCGATTTACAAg > 1:4295982/1‑71 (MQ=255)
gCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACaaac > 1:1914269/1‑69 (MQ=255)
gCCGCGCCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACaaac > 1:5474769/1‑69 (MQ=255)
ccGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCg > 1:4131440/1‑71 (MQ=255)
ccGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGT‑GCGACCTGCCCGAGCGGTGCGATTTACAAGCGt > 1:4347390/1‑71 (MQ=255)
gcgcCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACaa > 1:4792923/1‑66 (MQ=255)
gcgcCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACaa > 1:3909760/1‑66 (MQ=255)
gcgcCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACaa > 1:2057523/1‑66 (MQ=255)
gcCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTga < 1:1424948/70‑1 (MQ=255)
gcCTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaa < 1:4008683/71‑1 (MQ=255)
cTGTGCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGgcgct < 1:1982515/54‑2 (MQ=255)
tgtgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaag < 1:5114094/69‑1 (MQ=255)
gtgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaa > 1:3075944/1‑67 (MQ=255)
tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTAc < 1:2743499/57‑1 (MQ=255)
tgCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:5674081/69‑1 (MQ=255)
tgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCg > 1:2189811/1‑46 (MQ=12)
tgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa < 1:5420271/69‑1 (MQ=255)
tgCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCgaagaa < 1:1690358/69‑1 (MQ=255)
gCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCg > 1:2820836/1‑50 (MQ=255)
gCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCC‑AGCGGTGCg > 1:1934623/1‑49 (MQ=255)
gCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACaaa > 1:4783294/1‑58 (MQ=255)
gCGAACACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACaaa > 1:298998/1‑58 (MQ=255)
cGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCg < 1:4920128/60‑1 (MQ=255)
cGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTg > 1:1109417/1‑62 (MQ=255)
gAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAagaaga > 1:5406577/1‑68 (MQ=255)
aaCACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATgg > 1:2365420/1‑70 (MQ=255)
aaCACTGCCTCAATCCCAGC‑TGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAagaagacg < 1:809713/69‑3 (MQ=255)
cTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:4544790/61‑1 (MQ=255)
cTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCg < 1:3433784/71‑1 (MQ=255)
tGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATTGCATCGTCCt > 1:854048/1‑70 (MQ=255)
tGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATc > 1:1163641/1‑65 (MQ=255)
tGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCt > 1:5067144/1‑70 (MQ=255)
aa‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaag > 1:3137770/1‑52 (MQ=255)
aa‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGGGAAGAAGATGGCATCGTCCTGAt < 1:4859008/71‑1 (MQ=255)
cccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCg < 1:4446315/71‑1 (MQ=255)
ccGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:998695/51‑1 (MQ=255)
cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:3814880/51‑1 (MQ=255)
cGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAc < 1:2858466/71‑1 (MQ=255)
ggCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:2387615/50‑1 (MQ=255)
ggCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGAcc < 1:4683953/71‑1 (MQ=255)
cATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGaagaag < 1:4593058/48‑1 (MQ=255)
tgtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:2313650/45‑1 (MQ=255)
tgcgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGa > 1:2592635/4‑71 (MQ=255)
gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:1287185/44‑1 (MQ=255)
gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:1390108/44‑1 (MQ=255)
gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:5629327/44‑1 (MQ=255)
gtgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTgaagaa < 1:543383/44‑1 (MQ=255)
gtgtgGCGACCTGCCCGAGCGGGGCGATTTACAAGCGTgaagaa < 1:1740031/44‑1 (MQ=255)
gcgtgGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGa > 1:4385442/3‑70 (MQ=255)
tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:2016248/1‑70 (MQ=255)
tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:534635/1‑70 (MQ=255)
tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:1775170/1‑70 (MQ=255)
tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:4396349/1‑70 (MQ=255)
tgtgGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGAt > 1:456002/1‑70 (MQ=255)
gCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATaaa > 1:5052181/1‑69 (MQ=255)
cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTcc > 1:2232410/1‑47 (MQ=255)
cTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtg < 1:4317549/71‑1 (MQ=255)
gCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTg > 1:3817871/1‑47 (MQ=255)
cccGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggc < 1:512664/70‑1 (MQ=255)
cccAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATTGATCAGGATAAATGCCGtggc < 1:2476477/70‑1 (MQ=255)
cGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGtggctgg > 1:5396429/1‑71 (MQ=255)
|
ACACCTTCATGATGTATTTGCCGCGCCTGTGCGAACACTGCCTGAA‑CCCGGCATGTGTGGCGACCTGCCCGAGCGGTGCGATTTACAAGCGTGAAGAAGATGGCATCGTCCTGATCGACCAGGATAAATGCCGTGGCTGG > minE/835803‑835942
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
