Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,014,668 |
G→A |
25.0% |
S467S (AGC→AGT) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,014,668 | 0 | G | A | 25.0%
| 72.0
/ 22.0
| 44 | S467S (AGC→AGT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (14/19); new base A (4/7); total (18/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAA > minE/1014600‑1014734
|
gccgCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTgctgact > 1:2804282/4‑71 (MQ=255)
ccgCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTgctgactg > 1:1923016/3‑71 (MQ=255)
ccGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCgg > 1:1332908/1‑71 (MQ=255)
ggACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGtt < 1:3406118/71‑1 (MQ=255)
ccgccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTAcc > 1:1428085/4‑71 (MQ=255)
accGATACTGCCAAGGTAGAAGCCTCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGtt < 1:1485907/66‑1 (MQ=255)
ccGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCttt < 1:2124863/71‑1 (MQ=255)
aTACTGCCGAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGc > 1:4718648/1‑71 (MQ=255)
tACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCg < 1:584932/58‑1 (MQ=255)
ccgaGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCAt > 1:4711726/4‑71 (MQ=255)
cAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGt > 1:3420216/1‑54 (MQ=255)
cAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATg < 1:3314451/71‑1 (MQ=255)
aaGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGa < 1:4642660/71‑1 (MQ=255)
gTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGc > 1:9102/1‑60 (MQ=255)
gTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCa < 1:2046162/71‑1 (MQ=255)
tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGt < 1:3664711/49‑1 (MQ=255)
tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGt < 1:836799/49‑1 (MQ=255)
tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCa > 1:2626776/1‑70 (MQ=255)
tAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:887462/71‑1 (MQ=255)
tAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAt < 1:2955299/71‑1 (MQ=255)
aGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt > 1:2383907/1‑70 (MQ=255)
aGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg > 1:875607/1‑70 (MQ=255)
gAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg < 1:2329419/70‑2 (MQ=255)
aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtgc < 1:3357362/70‑3 (MQ=255)
aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg < 1:3328683/69‑2 (MQ=255)
aaGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCt < 1:530231/71‑1 (MQ=255)
cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5149872/71‑1 (MQ=255)
cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:4932291/71‑1 (MQ=255)
cgccgTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg > 1:4858543/1‑71 (MQ=255)
tgtTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:4868250/59‑1 (MQ=255)
tgtTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:4861554/59‑1 (MQ=255)
ttttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCAt < 1:4304837/57‑1 (MQ=255)
tttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTgcgc > 1:3348219/1‑71 (MQ=255)
ttttACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTc < 1:328727/41‑1 (MQ=255)
tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg < 1:2412482/70‑1 (MQ=255)
tACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTg > 1:118167/1‑70 (MQ=255)
cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGc < 1:3500963/69‑1 (MQ=255)
cAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCa < 1:1257073/70‑1 (MQ=255)
cATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGa > 1:1866316/1‑71 (MQ=255)
aTCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGAt > 1:785037/1‑71 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3708461/1‑44 (MQ=255)
cGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATAc > 1:3604764/1‑44 (MQ=255)
gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5604358/48‑1 (MQ=255)
gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCg < 1:5680458/48‑1 (MQ=255)
gTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCa > 1:72032/1‑61 (MQ=255)
aCCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACAt > 1:2767763/1‑70 (MQ=255)
gctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa > 1:3249287/1‑71 (MQ=255)
gctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGag > 1:472056/1‑70 (MQ=255)
ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTg < 1:349343/57‑1 (MQ=255)
ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGa < 1:3328560/69‑1 (MQ=255)
ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCAACATAAGa < 1:2458362/69‑1 (MQ=255)
ctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTa > 1:5578970/1‑66 (MQ=255)
ggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGc > 1:538187/1‑50 (MQ=255)
|
GCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAA > minE/1014600‑1014734
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
