Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,078 |
G→C |
36.0% |
Q331E (CAG→GAG) ‡ |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,078 | 0 | G | C | 36.0%
| 102.2
/ 98.0
| 104 | Q331E (CAG→GAG) ‡ | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (39/27); new base C (14/23); total (53/51) |
| Fisher's exact test for biased strand distribution p-value = 4.29e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 |
Only 100 of 105 total aligned reads displayed.
CTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGC > minE/1015012‑1015147
|
cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:3582069/71‑1 (MQ=255)
cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg > 1:1005824/1‑71 (MQ=255)
cTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg < 1:139382/71‑1 (MQ=255)
cTTTGCGCAGCTCTTCAGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACgg > 1:5533592/1‑71 (MQ=255)
tggcgcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGtt > 1:3669571/3‑71 (MQ=255)
tGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGtt > 1:1342141/1‑70 (MQ=255)
cgcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGa > 1:2236303/1‑70 (MQ=255)
cgcAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa < 1:4711421/70‑1 (MQ=255)
tctgCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGa < 1:1433269/66‑1 (MQ=255)
gcAGTTCTTGTGGAATGTACTGGCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGAtt < 1:458306/71‑2 (MQ=255)
cAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCt < 1:2214529/71‑1 (MQ=255)
ctctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCt > 1:748674/1‑68 (MQ=255)
tctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGc > 1:2771865/1‑71 (MQ=255)
ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:1180815/1‑71 (MQ=255)
ctTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCt > 1:3193210/1‑71 (MQ=255)
cGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa < 1:190477/71‑1 (MQ=255)
atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:1889572/1‑49 (MQ=255)
atatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGAc > 1:263020/1‑49 (MQ=255)
tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata < 1:4241631/68‑1 (MQ=255)
tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1601331/71‑1 (MQ=255)
tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:685870/1‑66 (MQ=255)
tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:3493630/1‑66 (MQ=255)
tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt < 1:562367/71‑1 (MQ=255)
ttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt > 1:5620087/1‑70 (MQ=255)
tggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGt > 1:3042698/3‑70 (MQ=255)
tGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTc > 1:4645775/1‑53 (MQ=255)
ggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTAGCTTTAATGTTACGGt > 1:5529572/2‑69 (MQ=255)
gCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag < 1:493611/71‑1 (MQ=255)
gCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:1544333/1‑71 (MQ=255)
ccTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag < 1:2585167/70‑1 (MQ=255)
cTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcagcg < 1:765686/71‑2 (MQ=255)
tGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGtt < 1:2352472/42‑1 (MQ=16)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag > 1:5459541/1‑70 (MQ=255)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2699696/1‑69 (MQ=255)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:3118813/1‑69 (MQ=255)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTA‑GGTcagcag > 1:2488653/1‑69 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:2269201/1‑54 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:3866169/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:4189154/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:4837800/71‑1 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:4853656/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTAGCTTTGATATTACGGTCagcaga < 1:489632/70‑1 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:5114168/1‑53 (MQ=255)
aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:2264516/1‑53 (MQ=255)
ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc < 1:2001343/70‑1 (MQ=255)
ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:943428/1‑69 (MQ=255)
tgtGTTCCAGTTTTTCGATGCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagc < 1:4692495/67‑1 (MQ=255)
gtGTTCCAGTTTTTCGATCCATATACCTTCGCGGTTGATTTTCGCTTTAATGTTACCGTCagcggagcag < 1:3984026/69‑1 (MQ=255)
gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:812238/69‑1 (MQ=255)
gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:2617848/69‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:1212523/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:4598355/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3690239/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:5354398/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCtt < 1:4131354/46‑1 (MQ=25)
tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCtt < 1:2805948/46‑1 (MQ=25)
gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:2179569/70‑1 (MQ=255)
gTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga < 1:1146097/70‑1 (MQ=255)
ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:3603061/1‑71 (MQ=255)
ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:1604107/1‑71 (MQ=255)
ttCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga < 1:1525592/71‑1 (MQ=255)
ttCCAGTTTTTCGATCCAGATACCTTAGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga < 1:4348262/71‑1 (MQ=255)
tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc > 1:3339198/1‑71 (MQ=255)
ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:5284528/70‑1 (MQ=255)
ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACg > 1:55464/1‑71 (MQ=255)
ccAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTa > 1:5324530/1‑50 (MQ=255)
ccAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACg > 1:1805971/1‑71 (MQ=255)
cAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGGCAGCGGAGCAGGAGACGc < 1:4591599/71‑1 (MQ=255)
aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagca > 1:2883009/1‑62 (MQ=255)
aGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:465666/41‑1 (MQ=19)
aGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCttt < 1:5193442/41‑1 (MQ=19)
gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCtttaa > 1:3872506/1‑40 (MQ=16)
gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:988283/62‑1 (MQ=255)
gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:3605047/62‑1 (MQ=255)
gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:1152191/62‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:1178992/56‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1659209/53‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:939032/53‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:4554022/53‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3194806/63‑1 (MQ=255)
ttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg > 1:610987/1‑41 (MQ=16)
ttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg > 1:3770352/1‑41 (MQ=16)
tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa > 1:4828660/1‑39 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagca > 1:195876/1‑58 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga > 1:1768927/1‑63 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg > 1:5106137/1‑70 (MQ=255)
cGATCCAGATACCTTCGGGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCTGGAGACGCCCATgcc < 1:959164/70‑1 (MQ=255)
cGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgcc > 1:631225/1‑70 (MQ=255)
gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccga < 1:2196846/71‑1 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:1112260/1‑46 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:1884346/1‑46 (MQ=255)
gATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca < 1:4953286/43‑1 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg > 1:2236504/1‑70 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCACATACCGACCGGGCAg > 1:2294138/1‑70 (MQ=255)
cccTCACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa < 1:3037613/71‑1 (MQ=255)
cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGCCCGGGCAGGAt > 1:5521129/1‑70 (MQ=255)
cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:3316979/1‑71 (MQ=255)
cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:4567470/1‑71 (MQ=255)
tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc > 1:2567914/1‑71 (MQ=255)
gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgag > 1:5123485/1‑44 (MQ=255)
|
CTTTGCGCAGCTCTTCCGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGC > minE/1015012‑1015147
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
