Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,100 |
A→G |
43.2% |
N323N (AAT→AAC) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,100 | 0 | A | G | 43.2%
| 35.4
/ 111.5
| 95 | N323N (AAT→AAC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (30/24); new base G (19/22); total (49/46) |
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCA > minE/1015033‑1015170
|
tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata < 1:4241631/68‑1 (MQ=255)
tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa < 1:1601331/71‑1 (MQ=255)
tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:685870/1‑66 (MQ=255)
tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata > 1:3493630/1‑66 (MQ=255)
tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt < 1:562367/71‑1 (MQ=255)
ttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt > 1:5620087/1‑70 (MQ=255)
tggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGt > 1:3042698/3‑70 (MQ=255)
ggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTAGCTTTAATGTTACGGt > 1:5529572/2‑69 (MQ=255)
gCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag < 1:493611/71‑1 (MQ=255)
gCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag > 1:1544333/1‑71 (MQ=255)
ccTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag < 1:2585167/70‑1 (MQ=255)
cTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcagcg < 1:765686/71‑2 (MQ=255)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag > 1:5459541/1‑70 (MQ=255)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:2699696/1‑69 (MQ=255)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:3118813/1‑69 (MQ=255)
ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTA‑GGTcagcag > 1:2488653/1‑69 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:4853656/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag < 1:4837800/71‑1 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:3866169/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:4189154/1‑71 (MQ=255)
gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTAGCTTTGATATTACGGTCagcaga < 1:489632/70‑1 (MQ=255)
ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc < 1:2001343/70‑1 (MQ=255)
ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag > 1:943428/1‑69 (MQ=255)
tgtGTTCCAGTTTTTCGATGCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagc < 1:4692495/67‑1 (MQ=255)
gtGTTCCAGTTTTTCGATCCATATACCTTCGCGGTTGATTTTCGCTTTAATGTTACCGTCagcggagcag < 1:3984026/69‑1 (MQ=255)
gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:2617848/69‑1 (MQ=255)
gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:812238/69‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:4598355/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:5354398/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3690239/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:1212523/71‑1 (MQ=255)
tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga < 1:19175/71‑1 (MQ=255)
gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:2179569/70‑1 (MQ=255)
gTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga < 1:1146097/70‑1 (MQ=255)
ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:3603061/1‑71 (MQ=255)
ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga > 1:1604107/1‑71 (MQ=255)
ttCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga < 1:1525592/71‑1 (MQ=255)
ttCCAGTTTTTCGATCCAGATACCTTAGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga < 1:4348262/71‑1 (MQ=255)
tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc > 1:3339198/1‑71 (MQ=255)
ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:5284528/70‑1 (MQ=255)
ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACg > 1:55464/1‑71 (MQ=255)
ccAGTTTTTCGATCCAGATCCCCTGACGGGTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc < 1:99413/70‑1 (MQ=255)
ccAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTa > 1:5324530/1‑50 (MQ=255)
ccAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACg > 1:1805971/1‑71 (MQ=255)
cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag < 1:425225/64‑1 (MQ=255)
cAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGGCAGCGGAGCAGGAGACGc < 1:4591599/71‑1 (MQ=255)
aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagca > 1:2883009/1‑62 (MQ=255)
gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:988283/62‑1 (MQ=255)
gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:1152191/62‑1 (MQ=255)
gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag < 1:3605047/62‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag < 1:1178992/56‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:939032/53‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:1659209/53‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag < 1:4554022/53‑1 (MQ=255)
tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga < 1:3194806/63‑1 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagca > 1:195876/1‑58 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga > 1:1768927/1‑63 (MQ=255)
tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg > 1:5106137/1‑70 (MQ=255)
cGATCCAGATACCTTCGGGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCTGGAGACGCCCATgcc < 1:959164/70‑1 (MQ=255)
cGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgcc > 1:631225/1‑70 (MQ=255)
gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccga < 1:2196846/71‑1 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:1112260/1‑46 (MQ=255)
ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca > 1:1884346/1‑46 (MQ=255)
ccAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccgaccg > 1:2889007/1‑71 (MQ=255)
gATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca < 1:4953286/43‑1 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg > 1:2236504/1‑70 (MQ=255)
ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCACATACCGACCGGGCAg > 1:2294138/1‑70 (MQ=255)
cccTCACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa < 1:3037613/71‑1 (MQ=255)
cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGCCCGGGCAGGAt > 1:5521129/1‑70 (MQ=255)
cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:4567470/1‑71 (MQ=255)
cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:3316979/1‑71 (MQ=255)
tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc > 1:2567914/1‑71 (MQ=255)
gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgag > 1:5123485/1‑44 (MQ=255)
cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg > 1:1368071/3‑42 (MQ=25)
cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg > 1:2607614/3‑42 (MQ=25)
gcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgcgc > 1:678811/2‑71 (MQ=255)
gcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgc < 1:3486527/68‑1 (MQ=255)
aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc < 1:5279903/48‑1 (MQ=255)
ggTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCg < 1:2419461/71‑1 (MQ=255)
gTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt < 1:1919808/71‑1 (MQ=255)
ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCa > 1:4669302/1‑46 (MQ=255)
ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCa > 1:2768811/1‑46 (MQ=255)
ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCa > 1:492989/1‑46 (MQ=255)
ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:938789/1‑70 (MQ=255)
ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:4734037/1‑70 (MQ=255)
tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2493085/1‑71 (MQ=255)
tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:1126322/1‑71 (MQ=255)
tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:4244530/1‑71 (MQ=255)
gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc > 1:5690256/1‑71 (MQ=255)
gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc > 1:5197910/1‑71 (MQ=255)
aTCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:4283364/1‑52 (MQ=255)
ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1891375/56‑1 (MQ=255)
ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGt < 1:519775/69‑1 (MQ=255)
cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa > 1:226671/1‑38 (MQ=255)
cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGt < 1:2708153/67‑1 (MQ=255)
gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc > 1:1503710/1‑64 (MQ=255)
gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:820874/70‑1 (MQ=255)
gATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGa < 1:5118496/70‑1 (MQ=255)
atatTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt > 1:5249545/1‑71 (MQ=255)
atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:4396330/1‑71 (MQ=255)
|
TATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCA > minE/1015033‑1015170
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
