Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,199 |
A→G |
29.0% |
G290G (GGT→GGC) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,199 | 0 | A | G | 29.0%
| 127.5
/ 64.6
| 93 | G290G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (26/40); new base G (12/15); total (38/55) |
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 115 total aligned reads displayed.
TACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGG > minE/1015129‑1015268
|
tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:5632792/1‑71 (MQ=255)
tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:4369366/1‑71 (MQ=255)
tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3721887/1‑71 (MQ=255)
cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:766822/71‑1 (MQ=255)
cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:3918109/71‑1 (MQ=255)
cgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:4751028/71‑1 (MQ=255)
gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3642065/1‑67 (MQ=255)
gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca > 1:1116641/1‑70 (MQ=255)
gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca > 1:4505366/1‑70 (MQ=255)
gaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:3716781/70‑1 (MQ=255)
cAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTgcg > 1:1080147/1‑70 (MQ=255)
aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:5593544/63‑1 (MQ=255)
ggATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:5354759/61‑1 (MQ=255)
ggATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgcgcgc < 1:4035730/71‑3 (MQ=255)
gATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:4296266/71‑1 (MQ=255)
gATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:5543269/71‑1 (MQ=255)
tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:1704618/1‑69 (MQ=255)
tGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGcc > 1:3596755/1‑69 (MQ=255)
cgcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAg < 1:3368764/70‑1 (MQ=255)
ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2376671/71‑1 (MQ=255)
gTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAc > 1:4623788/1‑68 (MQ=255)
tGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg < 1:2570077/56‑1 (MQ=255)
tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:4065708/1‑71 (MQ=255)
tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:2578522/1‑71 (MQ=255)
aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:1413253/1‑52 (MQ=255)
aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:4418975/71‑3 (MQ=255)
aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:5281815/71‑3 (MQ=255)
aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgt < 1:2940748/70‑2 (MQ=255)
cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:4456709/49‑1 (MQ=255)
cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:2038483/49‑1 (MQ=255)
cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:4795824/49‑1 (MQ=255)
gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:1041444/71‑1 (MQ=255)
gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:2163767/71‑1 (MQ=255)
gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:2535727/47‑1 (MQ=255)
gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:3620845/47‑1 (MQ=255)
gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:2529111/47‑1 (MQ=255)
tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:3543896/71‑1 (MQ=255)
tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:2860717/71‑1 (MQ=255)
tGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct < 1:2047092/71‑3 (MQ=255)
cAGGCGAATCACGGGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTgcg > 1:1171393/1‑71 (MQ=255)
gCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:2215510/40‑1 (MQ=255)
gCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:1577095/40‑1 (MQ=255)
gCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:2625634/40‑1 (MQ=255)
gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2447383/63‑1 (MQ=255)
gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2112087/63‑1 (MQ=255)
cGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:2560277/64‑1 (MQ=255)
gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:79475/1‑41 (MQ=255)
gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:2654855/71‑1 (MQ=255)
gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:1013409/71‑1 (MQ=255)
aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAg > 1:5381085/1‑51 (MQ=255)
aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1184460/1‑71 (MQ=255)
tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:4131335/70‑1 (MQ=255)
tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:3377907/71‑1 (MQ=255)
tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2457002/71‑1 (MQ=255)
tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2829265/71‑1 (MQ=255)
aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:187400/1‑71 (MQ=255)
aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:3904013/1‑71 (MQ=255)
cgcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:3186520/71‑1 (MQ=255)
cgcgAATTTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttca > 1:4392976/1‑55 (MQ=255)
cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:5358661/55‑1 (MQ=255)
cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:828371/55‑1 (MQ=255)
cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:2828699/55‑1 (MQ=255)
cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgggc > 1:813981/1‑61 (MQ=255)
gcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc > 1:2286505/1‑70 (MQ=255)
ggATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAAt > 1:2359578/1‑71 (MQ=255)
aTGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTc < 1:2320369/71‑1 (MQ=255)
aTGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAt < 1:1610945/49‑1 (MQ=255)
tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGa < 1:472575/41‑1 (MQ=255)
tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt < 1:2910283/71‑1 (MQ=255)
tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:2990150/1‑68 (MQ=255)
tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:5068033/1‑68 (MQ=255)
tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:287885/59‑1 (MQ=255)
tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3395182/59‑1 (MQ=255)
aGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTcc < 1:89427/70‑1 (MQ=255)
gCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAg < 1:5613530/71‑1 (MQ=255)
aGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgctg < 1:4441711/70‑4 (MQ=255)
aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt > 1:4026848/1‑62 (MQ=255)
aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt > 1:2533073/1‑62 (MQ=255)
gTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:5691118/1‑71 (MQ=255)
tATTTATCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:1991901/51‑1 (MQ=255)
tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:2963042/36‑2 (MQ=12)
tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:3916370/51‑1 (MQ=255)
tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:5644418/51‑1 (MQ=255)
tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:2062810/68‑1 (MQ=255)
tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:170607/68‑1 (MQ=255)
tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:5335744/68‑1 (MQ=255)
tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:4015888/71‑1 (MQ=255)
aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:4286222/1‑46 (MQ=255)
aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac > 1:2587251/1‑70 (MQ=255)
tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:548298/45‑1 (MQ=255)
tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3136481/45‑1 (MQ=255)
tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:2772074/43‑1 (MQ=255)
tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:791727/43‑1 (MQ=255)
tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt > 1:2086504/1‑55 (MQ=255)
tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAg > 1:4687202/1‑61 (MQ=255)
tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:5206192/1‑71 (MQ=255)
tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:4518929/1‑71 (MQ=255)
accaccAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCa > 1:1342216/1‑47 (MQ=255)
caccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3732206/40‑1 (MQ=255)
caccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCgg < 1:4782366/71‑1 (MQ=255)
|
TACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGG > minE/1015129‑1015268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
