Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,015,618 |
G→C |
15.0% |
P151A (CCG→GCG) |
fumA ← |
aerobic Class I fumarate hydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,015,618 | 0 | G | C | 15.0%
| 175.6
/ 20.4
| 73 | P151A (CCG→GCG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (36/26); new base C (6/5); total (42/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
AACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTT > minE/1015550‑1015686
|
aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:4405777/1‑71 (MQ=255)
aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:2116629/1‑71 (MQ=255)
aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:4497177/1‑71 (MQ=255)
aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:1751716/1‑71 (MQ=255)
aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggc > 1:2999858/1‑70 (MQ=255)
cATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGttt < 1:4754688/71‑1 (MQ=255)
aTAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGtttt < 1:2929639/71‑1 (MQ=255)
aTAAAGATCGATCTGCGCTGGCAAGTTGGTGCCGGTATTCACCTCTTTATACATATCCAGCGGCGCGtttt > 1:5242443/1‑71 (MQ=255)
tAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGtttt > 1:1929062/1‑70 (MQ=255)
aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:3881137/1‑70 (MQ=255)
aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:1177020/1‑70 (MQ=255)
aGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:4381132/1‑69 (MQ=255)
aGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:497651/1‑69 (MQ=255)
atcgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGt < 1:2303055/71‑1 (MQ=255)
cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa < 1:3757661/70‑1 (MQ=255)
cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:4642723/1‑70 (MQ=255)
cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAt > 1:4709590/1‑71 (MQ=255)
gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:3908554/71‑1 (MQ=255)
gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:465080/71‑1 (MQ=255)
gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:4079060/71‑1 (MQ=255)
gCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCt < 1:1982053/71‑1 (MQ=255)
aGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGttt < 1:2697873/48‑1 (MQ=255)
aGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAg < 1:1692001/62‑1 (MQ=255)
ttGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGat < 1:2594335/71‑1 (MQ=255)
ggTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTg > 1:4238739/1‑45 (MQ=255)
agTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata < 1:3778340/69‑1 (MQ=255)
gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:3461270/70‑1 (MQ=255)
gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:4143419/70‑1 (MQ=255)
gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:5581302/1‑69 (MQ=255)
gTGCCGGTGGTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:3454570/70‑1 (MQ=255)
gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:1775278/70‑1 (MQ=255)
gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:3564154/70‑1 (MQ=255)
ggTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:534164/1‑64 (MQ=255)
ggTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:1171213/1‑64 (MQ=255)
ggTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGgtgt < 1:4440195/71‑1 (MQ=255)
gTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc < 1:2761376/59‑1 (MQ=255)
gTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:4724312/71‑1 (MQ=255)
aTTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGa > 1:1563653/1‑39 (MQ=255)
aTTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTa > 1:171411/1‑52 (MQ=255)
ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:5054075/71‑1 (MQ=255)
tCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt > 1:294279/1‑67 (MQ=255)
cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc > 1:5493322/1‑54 (MQ=255)
cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:609669/1‑71 (MQ=255)
cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:1504845/1‑71 (MQ=255)
cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:2754096/1‑71 (MQ=255)
cttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCtt > 1:2849303/1‑51 (MQ=255)
cttcttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGAtata < 1:5037207/58‑1 (MQ=255)
cttTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcct > 1:3645695/1‑70 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:5226723/1‑41 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:5193590/1‑41 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:5365871/1‑41 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:5372689/1‑41 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:5617610/1‑41 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTAt > 1:3926092/1‑45 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTAt > 1:1387125/1‑45 (MQ=255)
cttTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTAt > 1:1919862/1‑45 (MQ=255)
tataCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACcgc < 1:4172446/69‑1 (MQ=255)
tataCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACcgc < 1:1219466/69‑1 (MQ=255)
gtaCATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:99921/2‑49 (MQ=255)
gtaCATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:3952652/2‑49 (MQ=255)
taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCgcgcgc > 1:4243451/1‑71 (MQ=255)
aCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGAtata < 1:301726/49‑1 (MQ=255)
aCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:3652130/55‑1 (MQ=255)
aCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:931394/55‑1 (MQ=255)
aCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:5163592/55‑1 (MQ=255)
aCATATCCAGCGGCGCGTTTTGCGACTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCgcgcgc > 1:3526384/1‑70 (MQ=255)
cATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa < 1:4582949/71‑1 (MQ=255)
atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc > 1:3748015/1‑39 (MQ=255)
atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:4993292/44‑1 (MQ=255)
atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:1678795/44‑1 (MQ=255)
atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAgcg > 1:3766262/1‑71 (MQ=255)
tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATaa > 1:3536829/1‑45 (MQ=255)
tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGaca > 1:3149096/1‑57 (MQ=255)
ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc > 1:1071204/1‑71 (MQ=255)
ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc > 1:5688093/1‑71 (MQ=255)
ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc > 1:1919440/1‑71 (MQ=255)
cAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgccg > 1:4531729/1‑71 (MQ=255)
cAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgccg > 1:967218/1‑71 (MQ=255)
gcggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGac < 1:760103/52‑1 (MQ=255)
cggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCtt < 1:2243750/71‑1 (MQ=255)
ggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGacac > 1:389997/1‑52 (MQ=255)
|
AACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTT > minE/1015550‑1015686
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
