Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,258,389 |
T→G |
23.6% |
intergenic (+60/+105) |
yecP → / ← torZ |
predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,258,389 | 0 | T | G | 23.6%
| 156.8
/ 49.8
| 88 | intergenic (+60/+105) | yecP/torZ | predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base T (25/37); new base G (8/10); total (41/47) |
| Fisher's exact test for biased strand distribution p-value = 7.91e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 |
GCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAT > minE/1258323‑1258458
|
gCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt > 1:4526559/1‑71 (MQ=255)
ccGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTc < 1:1501724/71‑1 (MQ=255)
gTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt < 1:927460/68‑1 (MQ=255)
gTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa < 1:5475036/71‑1 (MQ=255)
tAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa > 1:412870/1‑70 (MQ=255)
aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatat < 1:5024919/71‑1 (MQ=255)
aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:2229955/1‑70 (MQ=255)
aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:2076606/1‑70 (MQ=255)
ggTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatatat > 1:3478701/1‑71 (MQ=255)
tCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg < 1:1485836/71‑1 (MQ=255)
tCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg < 1:4844379/71‑1 (MQ=255)
tGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg > 1:3480889/1‑69 (MQ=255)
gTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg < 1:4004945/71‑1 (MQ=255)
ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt < 1:2476714/54‑1 (MQ=255)
ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata < 1:3528017/59‑1 (MQ=255)
ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAc > 1:4767619/1‑71 (MQ=255)
aCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata < 1:1564739/57‑1 (MQ=255)
tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata < 1:5606571/55‑1 (MQ=255)
ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg > 1:3734384/1‑69 (MQ=255)
ccGGATGCGGCGTGAACGCCTTATCCGACCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:4526893/69‑1 (MQ=255)
cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa < 1:1022153/49‑1 (MQ=255)
cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa < 1:857020/49‑1 (MQ=255)
ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAgg < 1:3870347/71‑1 (MQ=255)
gATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt < 1:2538819/45‑1 (MQ=255)
aTGCGGCGTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGcc > 1:1312535/1‑71 (MQ=2)
tGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata > 1:887837/1‑48 (MQ=255)
cggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTg < 1:3147214/70‑1 (MQ=255)
gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata < 1:5157068/44‑1 (MQ=11)
gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATaa < 1:3969092/71‑1 (MQ=2)
cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata < 1:2385923/43‑1 (MQ=14)
cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg > 1:2912678/1‑71 (MQ=9)
cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAg < 1:163709/71‑1 (MQ=9)
cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt > 1:1196526/1‑69 (MQ=2)
cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt > 1:5601232/1‑69 (MQ=2)
gTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAatata > 1:2725210/1‑44 (MQ=11)
gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg > 1:88908/1‑62 (MQ=2)
gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg > 1:4938926/1‑62 (MQ=2)
tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCa < 1:2533891/48‑1 (MQ=255)
tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg < 1:3963067/57‑1 (MQ=255)
aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGcg > 1:5304301/1‑68 (MQ=9)
aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGcg > 1:1237008/1‑68 (MQ=9)
aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa > 1:4045185/1‑37 (MQ=255)
aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg > 1:4206099/1‑48 (MQ=255)
aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg > 1:5130665/1‑48 (MQ=255)
aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg < 1:3962078/48‑1 (MQ=255)
aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt < 1:4433128/71‑1 (MQ=255)
aaCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg > 1:1345539/1‑69 (MQ=2)
aCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAg > 1:4359043/1‑71 (MQ=9)
aCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg > 1:4030252/1‑68 (MQ=2)
cGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata < 1:5378064/37‑1 (MQ=14)
cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa > 1:106423/1‑48 (MQ=255)
cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa > 1:5654816/1‑48 (MQ=255)
ccTTATCCGGCCTACAAATCCTTGCGAATTCAata > 1:3253930/1‑35 (MQ=14)
ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:1787019/1‑71 (MQ=255)
ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg > 1:3335205/1‑71 (MQ=255)
ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:955393/69‑1 (MQ=255)
ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg < 1:63454/69‑1 (MQ=255)
ttATCCGACCCACAAAGTCTTGCAAAATCAATAAATAGCAGGAACGATGTAGGCCTGATa > 1:935178/1‑60 (MQ=2)
aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCAt > 1:1791272/1‑69 (MQ=9)
aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa > 1:4474839/1‑69 (MQ=255)
aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:4121435/1‑70 (MQ=255)
aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt > 1:5182346/1‑70 (MQ=255)
tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:144553/43‑3 (MQ=14)
tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:3417786/43‑3 (MQ=14)
tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:4037079/43‑3 (MQ=14)
tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa < 1:3424206/43‑3 (MQ=14)
tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCATCAg < 1:4437046/71‑1 (MQ=9)
gCCTACAAATCCTTGCGAATTCAATATATTGTATGGA‑AATTCAGGCCTGATAAGCGTAGCGCATCa > 1:3288905/1‑66 (MQ=9)
gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:3345577/55‑1 (MQ=255)
gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg < 1:920956/55‑1 (MQ=255)
tACAAAACCTTGCTAATTCAATATATTGCAGGGACTAt < 1:4967192/38‑1 (MQ=255)
tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa < 1:2978919/61‑1 (MQ=255)
aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt < 1:1561115/54‑1 (MQ=255)
aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt > 1:4506173/1‑71 (MQ=255)
cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt < 1:3539309/44‑1 (MQ=255)
cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt < 1:2390062/44‑1 (MQ=255)
aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt < 1:959986/59‑1 (MQ=255)
aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt < 1:3053980/59‑1 (MQ=255)
aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCttt < 1:1847400/70‑1 (MQ=255)
aaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGc > 1:1623060/1‑48 (MQ=255)
aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt < 1:4203399/71‑1 (MQ=255)
aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt < 1:2336628/71‑1 (MQ=255)
ccTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGtt > 1:1082953/1‑71 (MQ=255)
tGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGttt > 1:1692566/1‑69 (MQ=255)
gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg > 1:3310184/1‑55 (MQ=255)
gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg > 1:2003473/1‑55 (MQ=255)
cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt < 1:3921158/71‑1 (MQ=255)
cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt < 1:3633435/71‑1 (MQ=255)
tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt > 1:3643659/1‑70 (MQ=255)
tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt > 1:2458677/1‑70 (MQ=255)
|
GCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAT > minE/1258323‑1258458
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
