Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,299,487 |
G→A |
62.0% |
intergenic (‑412/+78) |
cobU ← / ← yeeX |
bifunctional cobinamide kinase and cobinamide phosphate guanylyltransferase/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,299,487 | 0 | G | A | 62.0%
| 33.4
/ 47.6
| 50 | intergenic (‑412/+78) | cobU/yeeX | bifunctional cobinamide kinase and cobinamide phosphate guanylyltransferase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/6); new base A (15/16); total (28/22) |
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAA > minE/1299419‑1299556
|
ttGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgaggat > 1:2886363/1‑71 (MQ=255)
acaacGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACg > 1:2418501/1‑71 (MQ=255)
gAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAg < 1:4801208/71‑1 (MQ=255)
aTCCGGTATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGAt > 1:736262/1‑68 (MQ=255)
aTCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCggatgaggat < 1:4214647/48‑1 (MQ=255)
aTCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACCGTg > 1:4438504/1‑58 (MQ=255)
ggAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATCATGACGGTGATACACAGATAAGAATc < 1:3470771/71‑1 (MQ=255)
cGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaat < 1:2815251/71‑1 (MQ=255)
aCTATAATTTCGATTGGTTCTCGGATGAGGCtgatga < 1:734875/37‑1 (MQ=255)
gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGa > 1:4676791/3‑41 (MQ=255)
gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAg < 1:1265391/51‑1 (MQ=255)
gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAg < 1:16855/51‑1 (MQ=255)
gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagca > 1:4760695/3‑65 (MQ=255)
gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagca > 1:1578557/3‑65 (MQ=255)
gttaATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAgg > 1:3712144/3‑71 (MQ=255)
ttaATTTCGATTGGTTCTCGGATGAGAATGATGACgg < 1:1878994/36‑1 (MQ=255)
taATTTCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGt > 1:3682244/1‑71 (MQ=255)
aaTTTCGATTGGTTCTCGGATGAGAATGATGCCGGTg > 1:5558383/1‑37 (MQ=255)
tttCGATTGGTTCTCGGATGAGAATGATGACGGTGATa < 1:4485575/38‑1 (MQ=255)
tttCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAAt < 1:1576840/51‑1 (MQ=255)
tttCGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGc < 1:4329047/71‑1 (MQ=255)
ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:1891705/1‑71 (MQ=255)
ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:1879182/1‑71 (MQ=255)
ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:4659974/1‑71 (MQ=255)
ttCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGcc > 1:1138704/1‑71 (MQ=255)
tCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGa > 1:5229794/1‑42 (MQ=255)
tCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGa > 1:4813716/1‑42 (MQ=255)
tCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGa > 1:1811707/1‑42 (MQ=255)
cGATTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatga > 1:3828495/1‑61 (MQ=255)
aTTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGc < 1:3413670/71‑1 (MQ=255)
aTTGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGc < 1:2834904/71‑1 (MQ=255)
ttGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCa < 1:1119972/71‑1 (MQ=255)
tGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGc > 1:1126321/1‑64 (MQ=255)
tGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCaa > 1:668830/1‑71 (MQ=255)
tGGTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCa < 1:4331363/70‑1 (MQ=255)
ggTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCaa < 1:3194197/70‑1 (MQ=255)
ggTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCaa < 1:776394/70‑1 (MQ=255)
ggTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatgag > 1:2492974/1‑57 (MQ=255)
ggTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatgag > 1:3507679/1‑57 (MQ=255)
ggTTCTCGGATGAGAATGATGACGGTGATACACAGATAAGAATcaatgagcaatgag > 1:3660162/1‑57 (MQ=255)
ggTTCTCGGATGAGAATGATGACGGTGATACAAAGATAAGAATcaatgagcaatgag > 1:4400970/1‑57 (MQ=255)
ttCTCGGATGAGAATGATGACGGTGATACACAGATAAGAAt < 1:5176984/41‑1 (MQ=255)
ttCTCGGATGAGAATGATGACGGTGATACACAGATAAGAAt < 1:3817267/41‑1 (MQ=255)
tctcGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt > 1:4965426/1‑71 (MQ=255)
tctcGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt > 1:1631714/1‑71 (MQ=255)
ctcGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt > 1:4603600/1‑70 (MQ=255)
ctcGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCt < 1:325468/70‑1 (MQ=255)
cGGATGAGGATGATGACGGTGATACACAGATAAGAATcaatgagcaa > 1:275216/1‑47 (MQ=255)
cGGATGAGAATGATGACGGTGATACACAGATAAGAATc < 1:2150772/38‑1 (MQ=255)
cGGATGAGAATGATGACGGTGATACACAGATAAGAATc < 1:2144172/38‑1 (MQ=255)
cGGATGAGAATGATGACGGTGATACACAGATAAGAATc < 1:193263/38‑1 (MQ=255)
cGGATGAGAATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCg > 1:3113920/1‑59 (MQ=255)
gatgaggatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCAtt < 1:1463320/70‑1 (MQ=255)
gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:3938001/45‑1 (MQ=255)
gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:778066/45‑1 (MQ=255)
gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:2539479/45‑1 (MQ=255)
gatgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:4382338/45‑1 (MQ=255)
catgagaatgaTGACGGTGATACACAGATAAGAATcaatgagcaa < 1:5486510/44‑1 (MQ=255)
atgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGc < 1:3144439/54‑1 (MQ=255)
atgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGa < 1:2588942/71‑1 (MQ=255)
tgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGaa < 1:3050738/71‑1 (MQ=255)
tgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGaa < 1:1902230/71‑1 (MQ=255)
tgagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGa < 1:2489490/70‑1 (MQ=255)
gagaatgaTGACGGTGATACACAGATAAGAATcaa > 1:1941911/1‑35 (MQ=255)
gagaatgaTGACGGTGATACACAGATAAGAATcaa > 1:2783634/1‑35 (MQ=255)
gagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTg < 1:2689362/51‑1 (MQ=255)
gagaatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGaa > 1:867047/1‑70 (MQ=255)
ggatgaTGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATaa < 1:2367832/71‑1 (MQ=255)
|
TTGTAAAACAACGTAATAGAATTATCCGGAATGGTGGCGACTATAATTTCGATTGGTTCTCGGATGAGGATGATGACGGTGATACACAGATAAGAATCAATGAGCAATGAGGGTTGCCGGGCAACCCTCATTGAATAA > minE/1299419‑1299556
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
