Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,309,751 |
C→T |
29.7% |
V81I (GTC→ATC) |
yefM ← |
antitoxin of the YoeB‑YefM toxin‑antitoxin system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,309,751 | 0 | C | T | 29.7%
| 46.6
/ 23.3
| 37 | V81I (GTC→ATC) | yefM | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base C (14/12); new base T (5/6); total (19/18) |
| Fisher's exact test for biased strand distribution p-value = 7.28e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 |
CTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGG > minE/1309681‑1309821
|
cTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGAc > 1:3243563/1‑71 (MQ=255)
taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTcc > 1:1593833/1‑71 (MQ=255)
taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTcc > 1:2544970/1‑71 (MQ=255)
taAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTcc > 1:1707546/1‑71 (MQ=255)
taAGGCTACGCTAGCGTATCAAAAATGACAATTGATTCTATGAATGAATCTGTTCAATGACGTCCt < 1:100302/66‑1 (MQ=255)
aaGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCg > 1:3075804/1‑71 (MQ=255)
tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGcc > 1:4816987/1‑64 (MQ=255)
tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAttt > 1:282512/1‑70 (MQ=255)
tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAttt > 1:1602073/1‑70 (MQ=255)
tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAttt > 1:3920503/1‑70 (MQ=255)
tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGAtt > 1:4147445/1‑69 (MQ=255)
tCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTc < 1:2282396/71‑1 (MQ=255)
gACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTc < 1:4301870/44‑1 (MQ=255)
aCAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCg < 1:1675075/71‑1 (MQ=255)
cAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGa < 1:2773632/71‑1 (MQ=255)
cTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGa > 1:4887146/1‑66 (MQ=255)
tgaatgaaTCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATc > 1:5525881/1‑70 (MQ=255)
tgaatgaaTCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCa < 1:2355566/71‑1 (MQ=255)
aatCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTcc < 1:1021725/71‑1 (MQ=255)
tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAAtc < 1:3540320/64‑1 (MQ=255)
tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAAtc < 1:1820319/64‑1 (MQ=255)
tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAAtc < 1:3998463/64‑1 (MQ=255)
tGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATATc > 1:1311772/1‑39 (MQ=255)
actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGc > 1:2872641/3‑70 (MQ=255)
actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCg > 1:4832637/3‑71 (MQ=255)
actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCg > 1:4003314/3‑71 (MQ=255)
actCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCg > 1:1861607/3‑71 (MQ=255)
ttCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCt < 1:993843/42‑1 (MQ=255)
ttCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCt < 1:712842/42‑1 (MQ=255)
tCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGc < 1:2853134/40‑1 (MQ=255)
tCAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGc < 1:5224612/40‑1 (MQ=255)
cAATGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGc < 1:4518319/39‑1 (MQ=255)
aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGtt < 1:946480/69‑1 (MQ=255)
aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAg < 1:4862433/71‑1 (MQ=255)
aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAg < 1:2187059/71‑1 (MQ=255)
aaTGATGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTACGGGCGtt > 1:5191849/1‑69 (MQ=255)
atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATc < 1:1902720/41‑1 (MQ=255)
atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATc < 1:3847276/41‑1 (MQ=255)
atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATc < 1:2197681/41‑1 (MQ=255)
atgatgTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGt < 1:2612528/49‑1 (MQ=255)
tGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCg > 1:3194440/1‑71 (MQ=255)
gACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgg > 1:4129340/1‑71 (MQ=255)
cGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgggg < 1:42893/71‑1 (MQ=255)
cGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgggg < 1:1862645/71‑1 (MQ=255)
cGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCgggg < 1:2122161/71‑1 (MQ=255)
|
CTTAGATCTATAAGGCTACGCTAGCGTATCAAAACTGACAATTCATTCTATGAATGAATCTGTTCAATGACGTCCTTTTCCGTTCCTTTGCCTGATTTCAGGCTATCGATTGAGTCCATCAATCTCCGGGCGTTAGCGGGG > minE/1309681‑1309821
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
