Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,614,455 |
A→T |
31.3% |
intergenic (‑80/+137) |
eutH ← / ← eutG |
predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,614,455 | 0 | A | T | 31.3%
| 122.7
/ 72.1
| 83 | intergenic (‑80/+137) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base A (30/27); new base T (12/14); total (42/41) |
| Fisher's exact test for biased strand distribution p-value = 6.41e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
CCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTG > minE/1614387‑1614521
|
ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg < 1:865819/71‑1 (MQ=255)
ttAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg < 1:3206098/70‑1 (MQ=255)
ttatttatTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc > 1:3972471/1‑71 (MQ=255)
atttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcgc > 1:1815634/1‑71 (MQ=255)
atttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAcg < 1:5531776/70‑1 (MQ=255)
ttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCgg > 1:2696822/1‑71 (MQ=255)
ttGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGc > 1:3541707/1‑69 (MQ=255)
ctGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCa < 1:2234559/69‑1 (MQ=11)
tGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:1456548/1‑71 (MQ=39)
tGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTAGATAAGACGCGGCAAg > 1:4947173/1‑71 (MQ=255)
gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCg > 1:3857576/1‑71 (MQ=14)
aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGttg > 1:1982493/1‑68 (MQ=14)
tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCa < 1:3901698/71‑1 (MQ=14)
tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa < 1:4221789/71‑1 (MQ=39)
tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa < 1:3641343/71‑1 (MQ=39)
tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCa < 1:2416348/71‑1 (MQ=39)
cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATc > 1:2534512/1‑71 (MQ=14)
ggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATcc < 1:3777624/71‑1 (MQ=39)
ggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATcc < 1:2929521/71‑1 (MQ=39)
cgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCgg > 1:2523114/1‑71 (MQ=39)
gTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:4820010/71‑1 (MQ=14)
tAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGttg < 1:1639171/62‑3 (MQ=14)
tAAACGCCTTATCCGCACTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCa < 1:4381879/71‑1 (MQ=11)
aaaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCaa > 1:1555259/1‑71 (MQ=38)
gaCGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGcgt < 1:5543437/69‑3 (MQ=11)
aaCGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTc > 1:1030981/1‑59 (MQ=11)
aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:2938918/1‑70 (MQ=11)
aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:658721/1‑70 (MQ=11)
aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:671011/1‑70 (MQ=11)
aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:3353167/1‑70 (MQ=11)
aaCGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaac > 1:3491071/1‑70 (MQ=11)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:1797315/1‑71 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:2797065/1‑71 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt < 1:5201586/71‑1 (MQ=38)
aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3856953/1‑71 (MQ=38)
aCGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCatct > 1:1726505/1‑68 (MQ=11)
gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:38098/1‑70 (MQ=11)
gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:236485/1‑70 (MQ=11)
gCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTg > 1:5672081/1‑70 (MQ=11)
ccTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCa < 1:1479959/71‑1 (MQ=14)
ccTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCa < 1:3840454/71‑1 (MQ=14)
ccTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCa > 1:1914965/1‑71 (MQ=255)
cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:1635542/63‑1 (MQ=35)
ttATCCGCCCTACATGTGCAATTCCGTAACCTTGATAAAACGCGACAAGCg < 1:431/51‑1 (MQ=2)
ttATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCg < 1:378486/51‑1 (MQ=11)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:5187757/1‑64 (MQ=39)
tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:5126278/1‑69 (MQ=255)
aTCCGCCCTACATGTGTGTTCCCGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACcgc > 1:2750132/1‑71 (MQ=14)
aTCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:4944970/60‑1 (MQ=11)
aTCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:2264733/60‑1 (MQ=11)
aTCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGc < 1:793690/60‑1 (MQ=11)
aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAcc < 1:502711/69‑1 (MQ=255)
cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:4477590/41‑1 (MQ=9)
cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:4348493/41‑1 (MQ=9)
cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:1971297/41‑1 (MQ=9)
cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:401474/41‑1 (MQ=9)
cccTACATGTGCAATTCCGTAACCTGGATAAAACGCGACaa < 1:3316219/41‑1 (MQ=9)
cccTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCaa > 1:364833/1‑57 (MQ=2)
cccTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCAc < 1:4959017/71‑1 (MQ=255)
ccTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAg < 1:2669289/41‑1 (MQ=9)
tACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTc < 1:5638472/43‑1 (MQ=9)
tACATGTGCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGc < 1:502283/45‑1 (MQ=11)
tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:5323426/1‑71 (MQ=255)
tACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:3925711/1‑71 (MQ=255)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:3817910/71‑1 (MQ=14)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:807848/71‑1 (MQ=14)
cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg < 1:1671830/71‑1 (MQ=14)
cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGc > 1:587354/1‑38 (MQ=40)
cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg > 1:5282379/1‑39 (MQ=40)
cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg > 1:3073076/1‑39 (MQ=40)
cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCg > 1:3850333/1‑39 (MQ=40)
cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg < 1:4556734/71‑1 (MQ=255)
aTGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCg > 1:4747650/1‑70 (MQ=255)
gtgCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGcgt > 1:2478874/1‑47 (MQ=11)
gtgCAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGcgt > 1:2978124/1‑47 (MQ=11)
cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcg < 1:5055456/71‑1 (MQ=255)
cAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgc < 1:2173726/70‑1 (MQ=255)
aaTCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATgcgg < 1:3088174/71‑1 (MQ=255)
ccGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg < 1:988845/71‑1 (MQ=11)
ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:26239/1‑58 (MQ=255)
ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTgg > 1:1764484/1‑58 (MQ=255)
ccGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGt > 1:1779074/1‑70 (MQ=255)
cGTAGGTCGGATAAGACGCGACAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg > 1:4811753/1‑70 (MQ=11)
|
CCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTG > minE/1614387‑1614521
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
