Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,747,030 |
C→T |
20.5% |
intergenic (+114/+95) |
srmB → / ← yfiE |
ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,747,030 | 0 | C | T | 20.5%
| 162.7
/ 30.8
| 73 | intergenic (+114/+95) | srmB/yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (25/33); new base T (7/8); total (32/41) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 |
CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAT > minE/1746960‑1747099
|
cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATc < 1:947765/71‑1 (MQ=255)
cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATc < 1:5576898/71‑1 (MQ=255)
gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCt < 1:3997055/71‑1 (MQ=255)
aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:5417556/71‑1 (MQ=255)
aaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGt < 1:3822201‑M2/67‑1 (MQ=255)
aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGt < 1:1747399/71‑1 (MQ=255)
aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGt < 1:5319875/71‑1 (MQ=255)
aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATAAATTGTATTTGCACGATCTTGt < 1:2638081/71‑1 (MQ=255)
aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg > 1:1255450/1‑69 (MQ=255)
ccTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg < 1:3962906/71‑1 (MQ=255)
ccTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCg < 1:337807/71‑1 (MQ=14)
cTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg > 1:3841755/1‑70 (MQ=255)
tGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:5583573/62‑1 (MQ=14)
gATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGa > 1:2652614/1‑70 (MQ=14)
aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:4543787/70‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:4336819/70‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:2012413/70‑1 (MQ=14)
tGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATa < 1:5656641/70‑1 (MQ=255)
tGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa < 1:3724856/71‑1 (MQ=14)
cgcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAg < 1:537265/70‑1 (MQ=255)
cgcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:2101676/57‑1 (MQ=14)
cgcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:1601060/57‑1 (MQ=14)
gcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGc < 1:1839796/71‑1 (MQ=14)
cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg < 1:3888979/71‑1 (MQ=255)
cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg < 1:211605/71‑1 (MQ=255)
aCGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa > 1:4356994/1‑65 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGc > 1:2093406/1‑57 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGc > 1:3622613/1‑57 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:3952435/1‑70 (MQ=14)
cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:3799248/1‑70 (MQ=14)
cTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAc < 1:116695/71‑1 (MQ=255)
ttATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGtt < 1:1500793/67‑1 (MQ=255)
tATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTAcgc > 1:2397133/1‑71 (MQ=11)
cAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:2219218/1‑70 (MQ=255)
aGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:3075374/71‑1 (MQ=255)
ccTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAgg < 1:877077/55‑1 (MQ=255)
ccTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAgg < 1:5315591/55‑1 (MQ=255)
cTACGTGGCTCATGCAATATATTGAATTTGCACGATc > 1:2937576/1‑37 (MQ=255)
cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGc > 1:3182663/1‑52 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:4465721/1‑48 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:4243018/1‑48 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3191174/64‑1 (MQ=255)
gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:4993722/64‑1 (MQ=255)
tCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAt > 1:4811377/1‑70 (MQ=255)
tCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAt > 1:2646128/1‑70 (MQ=255)
aTGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATa > 1:952675/1‑69 (MQ=255)
tGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:2940977/50‑1 (MQ=255)
gCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:2895432/49‑1 (MQ=255)
cAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:5574907/1‑38 (MQ=255)
aaTATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:1655501/47‑1 (MQ=255)
tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa < 1:2996414/61‑1 (MQ=255)
atatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa > 1:3934492/1‑59 (MQ=255)
tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGtt < 1:4305556/39‑1 (MQ=40)
tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAgcg > 1:3753945/1‑66 (MQ=255)
tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAg > 1:2210189/1‑64 (MQ=255)
tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACtt > 1:3855468/1‑71 (MQ=255)
tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACtt > 1:2994516/1‑71 (MQ=255)
ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTc > 1:1032134/1‑38 (MQ=40)
ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:5161856/1‑39 (MQ=40)
ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:703815/1‑39 (MQ=40)
ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:5242872/1‑39 (MQ=40)
ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACttt < 1:1318810/70‑1 (MQ=255)
tGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgc > 1:5058831/1‑41 (MQ=38)
tGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgc > 1:1350355/1‑41 (MQ=38)
aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:4226580/1‑36 (MQ=40)
aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc < 1:909282/70‑1 (MQ=255)
tttGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGAc < 1:3447179/71‑1 (MQ=255)
ttGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGAc > 1:1737715/1‑70 (MQ=255)
tGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACa < 1:2079870/70‑1 (MQ=255)
aCGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGtt < 1:5129410/71‑1 (MQ=255)
cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACtt > 1:3331635/1‑58 (MQ=255)
cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:3360371/71‑1 (MQ=255)
cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:695727/71‑1 (MQ=255)
gATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:909579/70‑1 (MQ=255)
tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:3397571/68‑1 (MQ=255)
tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAt > 1:3960700/1‑71 (MQ=255)
|
CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAT > minE/1746960‑1747099
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
