Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,934,236 G→C 25.8% A69A (GCC→GCG) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,934,2360GC25.8% 151.3 / 59.1 93A69A (GCC→GCG) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (34/35);  new base C (16/8);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 101 total aligned reads displayed.

CCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGT  >  minE/1934168‑1934303
                                                                    |                                                                   
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg                                                                    >  1:1209557/1‑70 (MQ=255)
ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc                                                                   <  1:587097/71‑1 (MQ=255)
    cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg                                                                     >  1:5169788/1‑65 (MQ=255)
    cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg                                                                     >  1:5157556/1‑65 (MQ=255)
        cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca                                                            <  1:3961549/70‑2 (MQ=255)
         tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa                                                           >  1:5539454/1‑70 (MQ=255)
              ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa                                                     <  1:5377813/71‑1 (MQ=255)
                 aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg                                                  >  1:4978297/1‑71 (MQ=255)
                    gAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt                                                >  1:2343918/1‑70 (MQ=255)
                     aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                              >  1:3316697/1‑71 (MQ=255)
                      aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc                                             <  1:2878285/71‑1 (MQ=255)
                         aggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt                                          <  1:4023622/69‑1 (MQ=255)
                          ggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGtt                                         >  1:4066856/2‑71 (MQ=255)
                           gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt                                                      <  1:2252670/57‑2 (MQ=255)
                           gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                        >  1:2613270/1‑71 (MQ=255)
                             tttCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct                                      >  1:3144320/1‑69 (MQ=255)
                             tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt                                                       <  1:1295208/54‑1 (MQ=255)
                               tACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc                                     <  1:2759822/70‑1 (MQ=255)
                                aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg                                    <  1:776813/70‑1 (MQ=255)
                                 cccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttta                                                     >  1:5115961/1‑50 (MQ=255)
                                 cccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttta                                                     >  1:3147290/1‑50 (MQ=255)
                                 cccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttta                                                     >  1:3140687/1‑50 (MQ=255)
                                 cccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt                                  <  1:4873818/71‑1 (MQ=255)
                                  ccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt                                                      >  1:4023336/1‑49 (MQ=255)
                                  ccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt                                                      >  1:4348514/1‑49 (MQ=255)
                                  ccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGa                                                                 >  1:3181525/1‑39 (MQ=255)
                                   cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGt                                                        <  1:903255/47‑1 (MQ=255)
                                   cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCtt                                               >  1:4280273/1‑56 (MQ=255)
                                   cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct                                <  1:321230/71‑1 (MQ=255)
                                   cATAATACGACGGGTCATCATGCGGCCAGCAACCGCGACTTCGATGt                                                        <  1:4577549/47‑1 (MQ=255)
                                     taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                              <  1:2739660/55‑1 (MQ=255)
                                     taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                              <  1:2634463/55‑1 (MQ=255)
                                     taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                              <  1:2608632/55‑1 (MQ=255)
                                     taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt                                  <  1:1588650/67‑1 (MQ=255)
                                     taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct                              >  1:2161738/1‑71 (MQ=255)
                                      aataCGACGGGTCATCATGCGGCCAGCAACGGCGa                                                                 >  1:2908312/1‑35 (MQ=255)
                                      aataCGACGGGTCATCATGCGGCCAGCAACGGCGa                                                                 >  1:5406354/1‑35 (MQ=255)
                                      aataCGACGGGTCATCATGCGGCCAGCAACGGCGa                                                                 >  1:699434/1‑35 (MQ=255)
                                      aataCGACGGGTCATCATGCGGCCAGCAACGGCGa                                                                 >  1:2969353/1‑35 (MQ=255)
                                      aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt                             >  1:740975/1‑71 (MQ=255)
                                      aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt                             >  1:386593/1‑71 (MQ=255)
                                       ataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt                            >  1:435370/1‑71 (MQ=255)
                                       ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa                                                           >  1:1455992/1‑40 (MQ=255)
                                       ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                            >  1:5287180/1‑71 (MQ=255)
                                       ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                            >  1:3534875/1‑71 (MQ=255)
                                       ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                            <  1:846781/71‑1 (MQ=255)
                                       ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                            >  1:2285625/1‑71 (MQ=255)
                                        taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc                           >  1:5462633/1‑70 (MQ=255)
                                        taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc                           >  1:666016/1‑70 (MQ=255)
                                          cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCa                                            <  1:5205759/52‑1 (MQ=255)
                                          cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGcc                         <  1:2983383/71‑1 (MQ=255)
                                          cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc                          <  1:4796101/70‑1 (MQ=255)
                                          cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc                          <  1:3307086/70‑1 (MQ=255)
                                              gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg                     <  1:3866528/71‑1 (MQ=255)
                                                gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa                   <  1:1442612/71‑1 (MQ=255)
                                                  catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt                                      <  1:1445814/50‑1 (MQ=255)
                                                  catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt                  >  1:618336/1‑70 (MQ=255)
                                                   atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt                                          <  1:4439579/45‑1 (MQ=255)
                                                   atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt                            >  1:4256860/1‑59 (MQ=255)
                                                   atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt                            >  1:555288/1‑59 (MQ=255)
                                                   atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt                 <  1:686803/70‑1 (MQ=255)
                                                    tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                                              <  1:2284141/40‑1 (MQ=255)
                                                    tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTc                      <  1:3931232/64‑1 (MQ=255)
                                                    tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt               <  1:4701572/71‑1 (MQ=255)
                                                    taatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt                            >  1:5677460/3‑58 (MQ=255)
                                                     catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc                                       <  1:4904945/46‑2 (MQ=255)
                                                     catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc                                       <  1:4911708/46‑2 (MQ=255)
                                                     catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc                                       <  1:5380309/46‑2 (MQ=255)
                                                     catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                        >  1:1633243/1‑45 (MQ=255)
                                                     catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                        >  1:4980481/1‑45 (MQ=255)
                                                     catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc                                        >  1:4986892/1‑45 (MQ=255)
                                                     catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa                    <  1:4416385/65‑1 (MQ=255)
                                                     catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg              >  1:4585570/1‑71 (MQ=255)
                                                      atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg                                    >  1:5287455/1‑48 (MQ=255)
                                                      atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt                                  >  1:5369954/1‑50 (MQ=255)
                                                      atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg              <  1:923928/70‑1 (MQ=255)
                                                      atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg              <  1:4031458/70‑1 (MQ=255)
                                                      atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc             >  1:2425460/1‑71 (MQ=255)
                                                      atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCCTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg              <  1:4652666/70‑1 (MQ=255)
                                                      atGAGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg                                    >  1:4521076/1‑48 (MQ=255)
                                                        gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt           >  1:4291881/1‑71 (MQ=255)
                                                        gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt           >  1:17456/1‑71 (MQ=255)
                                                          ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTg          <  1:1099486/70‑1 (MQ=255)
                                                          ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGc         <  1:3994730/71‑1 (MQ=255)
                                                            ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTg                           <  1:5267452/51‑1 (MQ=255)
                                                            ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa       >  1:48892/1‑71 (MQ=255)
                                                               gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg    >  1:714320/1‑71 (MQ=255)
                                                                cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa                   >  1:3238388/1‑55 (MQ=255)
                                                                cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa                   >  1:2577827/1‑55 (MQ=255)
                                                                 aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt  >  1:3061736/1‑71 (MQ=255)
                                                                 aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt  <  1:807830/71‑1 (MQ=255)
                                                                 aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt  <  1:2595652/71‑1 (MQ=255)
                                                                 aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt  >  1:3421109/1‑71 (MQ=255)
                                                                  aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt  <  1:3417545/70‑1 (MQ=255)
                                                                  aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTATGCGTGCAATTGGt  >  1:1375951/1‑70 (MQ=255)
                                                                    ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc                               <  1:2081012/39‑1 (MQ=255)
                                                                    ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc                               <  1:5345666/39‑1 (MQ=255)
                                                                    ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa       >  1:700856/1‑63 (MQ=255)
                                                                    ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa       >  1:3461357/1‑63 (MQ=255)
                                                                    ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa       >  1:1073703/1‑63 (MQ=255)
                                                                    |                                                                   
CCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGT  >  minE/1934168‑1934303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A