Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,943,198 |
C→T |
21.8% |
T90I (ACA→ATA) ‡ |
bglA → |
6‑phospho‑beta‑glucosidase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,943,198 | 0 | C | T | 21.8%
| 213.0
/ 52.1
| 110 | T90I (ACA→ATA) ‡ | bglA | 6‑phospho‑beta‑glucosidase A |
| Reads supporting (aligned to +/- strand): ref base C (42/44); new base T (9/15); total (51/59) |
| Fisher's exact test for biased strand distribution p-value = 3.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 |
Only 100 of 116 total aligned reads displayed.
GTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTC > minE/1943128‑1943268
|
gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc > 1:4981092/1‑71 (MQ=255)
ttttATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAt > 1:2646213/1‑71 (MQ=255)
gTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTg < 1:543105/70‑1 (MQ=255)
ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCa > 1:3619433/1‑57 (MQ=255)
ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcgc > 1:2506636/1‑69 (MQ=255)
aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:3264055/1‑71 (MQ=255)
ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt < 1:1477950/71‑1 (MQ=255)
ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt > 1:764619/1‑71 (MQ=255)
ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGACGCGAAttt < 1:2554187/71‑1 (MQ=255)
ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:4451693/51‑1 (MQ=255)
ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:2639763/51‑1 (MQ=255)
aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt < 1:1327729/48‑1 (MQ=255)
aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt > 1:2585227/1‑71 (MQ=255)
aCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACgcgc < 1:3753628/62‑1 (MQ=255)
cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:2737300/71‑1 (MQ=255)
cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:3915062/71‑1 (MQ=255)
cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1202158/71‑1 (MQ=255)
cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1660478/71‑1 (MQ=255)
tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa < 1:2251707/71‑1 (MQ=255)
cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:3208253/42‑1 (MQ=255)
cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:5078748/1‑69 (MQ=255)
cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:1561382/1‑69 (MQ=255)
aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1166436/1‑52 (MQ=255)
aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2398283/1‑52 (MQ=255)
aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1521333/1‑52 (MQ=255)
aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:5079192/1‑71 (MQ=255)
aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3382894/1‑71 (MQ=255)
aaGCTATTTGCCAAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2704324/1‑52 (MQ=255)
ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGc < 1:2787904/43‑1 (MQ=255)
ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2860158/45‑1 (MQ=255)
ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2951430/45‑1 (MQ=255)
ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:1014966/45‑1 (MQ=255)
ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:1751890/45‑1 (MQ=255)
ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2433945/45‑1 (MQ=255)
ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTg > 1:2378438/2‑47 (MQ=255)
aTTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:2223538/1‑69 (MQ=255)
aTTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt > 1:2868506/1‑70 (MQ=255)
tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt < 1:4656174/56‑1 (MQ=255)
tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGacg > 1:2557695/1‑68 (MQ=255)
tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5418485/1‑68 (MQ=255)
tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5424863/1‑68 (MQ=255)
tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5500840/55‑1 (MQ=255)
tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5487623/55‑1 (MQ=255)
tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5689610/59‑1 (MQ=255)
tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:2761226/70‑1 (MQ=255)
tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:782863/70‑1 (MQ=255)
tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:2378942/71‑1 (MQ=255)
tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:1182698/70‑1 (MQ=255)
tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:373058/70‑1 (MQ=255)
tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:2888843/1‑66 (MQ=255)
tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg > 1:5497366/1‑71 (MQ=255)
gCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtt > 1:1556532/1‑51 (MQ=255)
ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:5681630/68‑1 (MQ=255)
ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:3346741/68‑1 (MQ=255)
ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAgtc < 1:1774428/71‑3 (MQ=255)
ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt < 1:4395735/65‑1 (MQ=255)
ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc < 1:3439558/70‑1 (MQ=255)
ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:132510/71‑1 (MQ=255)
gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:1439941/1‑69 (MQ=255)
gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:3975968/1‑69 (MQ=255)
aaTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGc > 1:3092252/1‑71 (MQ=255)
tGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGcgacga < 1:2414136/61‑1 (MQ=255)
gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa > 1:4804842/1‑70 (MQ=255)
gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa > 1:40758/1‑70 (MQ=255)
ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:3531599/70‑1 (MQ=255)
ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:3538224/70‑1 (MQ=255)
gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:4422594/57‑1 (MQ=255)
gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:5446878/57‑1 (MQ=255)
gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:5023357/69‑1 (MQ=255)
gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCACATTTTTCCAAAAGGCGATg < 1:1586141/57‑1 (MQ=255)
cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:4405322/41‑1 (MQ=255)
cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:2040899/41‑1 (MQ=255)
cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:293134/41‑1 (MQ=255)
tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5401820/43‑1 (MQ=255)
tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5015099/43‑1 (MQ=255)
cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3078846/1‑48 (MQ=255)
cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACg > 1:4576113/1‑68 (MQ=255)
aaTGTTTTCGTACATCCATTGCCTGGAGGCGCATTTTTCCAAAAGGCGATGAAGCTCAGcc > 1:3087242/1‑61 (MQ=255)
aaTGTTCTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAg > 1:12434/1‑45 (MQ=255)
aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc > 1:757194/1‑56 (MQ=255)
aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc > 1:4532978/1‑56 (MQ=255)
tGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAgg > 1:5491372/1‑71 (MQ=255)
gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:3968923/1‑46 (MQ=255)
gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:4443691/1‑46 (MQ=255)
gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg > 1:828286/1‑71 (MQ=255)
gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAgg > 1:5163031/1‑70 (MQ=255)
tttCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCt > 1:5520228/1‑71 (MQ=255)
tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:5515062/49‑1 (MQ=255)
tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg < 1:4410441/70‑1 (MQ=255)
tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg > 1:2610421/1‑70 (MQ=255)
tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg > 1:452740/1‑70 (MQ=255)
aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg < 1:3188849/37‑1 (MQ=255)
aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg < 1:5414125/37‑1 (MQ=255)
aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt > 1:35992/1‑71 (MQ=255)
aCATCCATTGCCTGGACGCGCATTTATCCAAAAGGCg < 1:3012323/37‑1 (MQ=255)
cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga < 1:3792148/58‑1 (MQ=255)
cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGttg < 1:3918682/71‑2 (MQ=255)
cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc < 1:410016/71‑1 (MQ=255)
cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc > 1:5297251/1‑71 (MQ=255)
cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc > 1:4727373/1‑71 (MQ=255)
|
GTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTC > minE/1943128‑1943268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
