Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,294,050 |
A→C |
56.5% |
intergenic (+77/‑198) |
gldA → / → yijF |
glycerol dehydrogenase, NAD/conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,294,050 | 0 | A | C | 56.5%
| 57.3
/ 120.2
| 115 | intergenic (+77/‑198) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (24/26); new base C (30/35); total (54/61) |
| Fisher's exact test for biased strand distribution p-value = 8.53e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01 |
Only 100 of 119 total aligned reads displayed.
CAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCA > minE/2293981‑2294117
|
cAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCaa > 1:2816222/1‑71 (MQ=255)
aCTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtct > 1:1456124/1‑71 (MQ=255)
cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:147098/66‑1 (MQ=255)
cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:3503152/66‑1 (MQ=255)
cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:5360329/66‑1 (MQ=255)
ggCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCa > 1:4320852/1‑70 (MQ=255)
ttGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAata > 1:4860091/1‑70 (MQ=255)
ttGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAata > 1:1397808/1‑70 (MQ=255)
gggAGTTTGAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTg < 1:2340155/69‑1 (MQ=255)
ggAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTg < 1:650623/68‑1 (MQ=255)
aGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt < 1:3568451/69‑1 (MQ=255)
gTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTg > 1:2191214/1‑71 (MQ=255)
tttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata < 1:327036/61‑1 (MQ=255)
ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAt > 1:3972747/1‑48 (MQ=255)
ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAt > 1:5078741/1‑48 (MQ=255)
cGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtt > 1:3491545/1‑71 (MQ=255)
cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCaa < 1:3392733/51‑1 (MQ=14)
cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:4396510/71‑1 (MQ=255)
cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:2258950/71‑1 (MQ=255)
cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:3711802/71‑1 (MQ=255)
gCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAat > 1:4557979/1‑51 (MQ=14)
gCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCttt < 1:4666928/71‑1 (MQ=255)
cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGc > 1:3312632/1‑63 (MQ=255)
aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:5264793‑M2/1‑70 (MQ=255)
aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGATTTTGCGTGCTTTTg > 1:5198063‑M2/1‑70 (MQ=255)
aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:1393426‑M2/71‑2 (MQ=255)
aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:5487399‑M2/71‑2 (MQ=255)
aaaaTGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:44739‑M2/70‑2 (MQ=255)
aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:4476929/59‑1 (MQ=255)
aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:4161666/59‑1 (MQ=255)
aaatGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:3981576/4‑69 (MQ=255)
aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGa < 1:4782298/54‑1 (MQ=255)
aatgcCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta > 1:2619056‑M2/3‑67 (MQ=255)
atGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGt > 1:2619201/2‑60 (MQ=255)
atGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTCCGt > 1:4345039/2‑60 (MQ=14)
tGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGc < 1:889139/71‑1 (MQ=255)
tGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGc < 1:557875/71‑1 (MQ=255)
tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgtagg > 1:223170‑M2/1‑61 (MQ=255)
tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:1324775/1‑71 (MQ=255)
tGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGa > 1:2831294/1‑71 (MQ=255)
gATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:4901081/1‑70 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAAt < 1:3007375/47‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:4455300/70‑1 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt > 1:3667451/1‑47 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:197334‑M2/1‑59 (MQ=255)
aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt > 1:1621580/1‑70 (MQ=255)
tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:3144606‑M2/60‑3 (MQ=255)
tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:2117498‑M2/60‑3 (MQ=255)
tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:5008087‑M2/60‑3 (MQ=255)
tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATaa < 1:4767790/71‑1 (MQ=255)
gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:4240040‑M2/60‑4 (MQ=255)
gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:5430285‑M2/60‑4 (MQ=255)
gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:900149‑M2/60‑4 (MQ=255)
gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:2471523‑M2/60‑4 (MQ=255)
gcgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc > 1:1789694/1‑53 (MQ=255)
cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:2057525/70‑1 (MQ=255)
cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTg < 1:2732235/45‑1 (MQ=255)
cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:5571167/54‑1 (MQ=255)
cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:4523524/54‑1 (MQ=255)
cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:1730447‑M2/57‑4 (MQ=255)
cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:1753831‑M2/57‑4 (MQ=255)
cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGaa < 1:4871911/41‑1 (MQ=255)
cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:1017138/71‑1 (MQ=255)
cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:581835/71‑1 (MQ=255)
tACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc < 1:234742/49‑1 (MQ=255)
aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGa > 1:527559/1‑38 (MQ=255)
aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCg > 1:2907694/1‑45 (MQ=255)
aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCg > 1:270371/1‑45 (MQ=255)
aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:465315‑M2/54‑3 (MQ=255)
aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:4276195/1‑46 (MQ=255)
aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:1090727/1‑46 (MQ=255)
aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:3674642/1‑46 (MQ=255)
cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:4114046/70‑1 (MQ=255)
cTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCAc > 1:3613057/1‑71 (MQ=255)
tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:54883/64‑1 (MQ=255)
tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:2644355/64‑1 (MQ=255)
aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc > 1:1376865/1‑71 (MQ=255)
tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:4811485‑M2/1‑45 (MQ=255)
tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:1119658‑M2/1‑45 (MQ=255)
tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGcag > 1:5440119/1‑51 (MQ=255)
tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGtt < 1:1285377/64‑1 (MQ=255)
tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:2993628/45‑1 (MQ=255)
tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:1718412/45‑1 (MQ=255)
cAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCg > 1:4720792/1‑70 (MQ=255)
aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:1977081/1‑56 (MQ=17)
aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:592788/1‑56 (MQ=17)
aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCa < 1:3097563/71‑1 (MQ=255)
gCCTACGCCATCTCTGCAATATATTGAATTTGCTTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATc < 1:5565589/71‑1 (MQ=14)
cTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:4650485/1‑52 (MQ=14)
cTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCg > 1:3390699/1‑71 (MQ=11)
tACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta > 1:5455641‑M2/1‑38 (MQ=255)
aCGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:776566/48‑1 (MQ=255)
cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:2887121/1‑49 (MQ=14)
cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:827485/1‑49 (MQ=14)
cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCgg < 1:2120277/69‑1 (MQ=11)
cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCgg < 1:1258126/69‑1 (MQ=11)
cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:4381388/1‑71 (MQ=11)
cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:2113870/1‑71 (MQ=11)
cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3472742/1‑71 (MQ=11)
cGCAATCTCTGCAATATAATGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCTTCCgg < 1:4109549/69‑1 (MQ=255)
|
CAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCA > minE/2293981‑2294117
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
