Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I0 R1
|
2892 |
75.3 |
5698161 |
66.1% |
3766484 |
59.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,415,812 |
G→T |
23.8% |
noncoding (67/77 nt) |
proM ← |
tRNA‑Pro |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,415,812 | 0 | G | T | 23.8%
| 99.2
/ 16.8
| 42 | noncoding (67/77 nt) | proM | tRNA‑Pro |
| Reads supporting (aligned to +/- strand): ref base G (18/14); new base T (5/5); total (23/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 |
GGAGGGTGACGAAATGCACAGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGA > minE/2415746‑2415879
|
ggagggTGACGAAATGCACAGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGagagag < 1:694645/71‑1 (MQ=255)
gTGACGAAATGCACAGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGa < 1:3141884/68‑1 (MQ=255)
gCACAGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTCGTCGGCGAGAGAGGATTCGAAc > 1:657077/1‑65 (MQ=255)
cacaGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAc > 1:5593864/1‑71 (MQ=255)
cacaGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAc > 1:1024987/1‑71 (MQ=255)
acaGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAcc < 1:4330351/71‑1 (MQ=255)
acaGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAcc < 1:3691230/71‑1 (MQ=255)
caGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAccc < 1:4726192/71‑1 (MQ=255)
caGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAccc < 1:4732647/71‑1 (MQ=255)
caGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAcc > 1:3209176/1‑70 (MQ=255)
gAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCAc > 1:2324492/1‑71 (MQ=255)
aCAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCa > 1:4755699/1‑66 (MQ=255)
aCAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTgg > 1:548331/1‑70 (MQ=255)
aaaaCCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTccc > 1:3533273/1‑70 (MQ=255)
aaaaCCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCa < 1:4770576/71‑1 (MQ=255)
aCCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGAcc < 1:5360046/57‑1 (MQ=255)
ccgccgAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGa < 1:4475856/44‑1 (MQ=255)
gAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgcg > 1:3122587/1‑71 (MQ=255)
gAAGCGGGGTTAAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgcg > 1:3793516/1‑71 (MQ=255)
gCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgcg > 1:577056/1‑68 (MQ=255)
gggTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAAcc < 1:5097850/58‑1 (MQ=255)
ttttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCag > 1:3108423/3‑70 (MQ=12)
ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCag < 1:2367851/69‑2 (MQ=12)
ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGc > 1:5014009/1‑71 (MQ=12)
ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGc > 1:468949/1‑71 (MQ=12)
ttCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTgc < 1:4029939/61‑1 (MQ=255)
cAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:3907592/1‑63 (MQ=255)
cAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTg > 1:4132185/1‑71 (MQ=255)
aaaaTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCa > 1:1731257/1‑39 (MQ=255)
aaaaTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCa < 1:1771869/53‑1 (MQ=255)
tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgcc < 1:1308051/70‑2 (MQ=16)
tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgcc < 1:4180925/70‑2 (MQ=16)
tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg > 1:2772012/2‑69 (MQ=16)
tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg > 1:1922640/2‑69 (MQ=16)
aTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:4671372/1‑59 (MQ=255)
aTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:1358257/1‑59 (MQ=255)
aTTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTa > 1:2281846/1‑59 (MQ=255)
ttGGTCGGTGATAGAGGATTCGAACCTCCGAcccc < 1:1540883/35‑2 (MQ=18)
ttGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAAcc < 1:1723109/48‑1 (MQ=255)
ttGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAAcc < 1:4283347/48‑1 (MQ=255)
ggTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTAc < 1:3823222/57‑1 (MQ=12)
ggCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGcc < 1:1147009/71‑1 (MQ=255)
gCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGcc > 1:1599202/1‑70 (MQ=255)
gagagaGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGa > 1:3291001/1‑70 (MQ=255)
|
GGAGGGTGACGAAATGCACAGAAAACAAAAAACCCCGCCGAAGCGGGGTTCAAAATTGGTCGGCGAGAGAGGATTCGAACCTCCGACCCACTGGTCCCAAACCAGTTGCGCTACCAAGCTGCGCTACTCGCCGA > minE/2415746‑2415879
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
