Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,569,871 G→T 33.5% A47A (GCG→GCT)  yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,569,8710GT33.5% 191.3 / 95.1 128A47A (GCG→GCT) yibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (37/48);  new base T (22/21);  total (59/69)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

Only 100 of 138 total aligned reads displayed.

GATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTC  >  minE/2569802‑2569934
                                                                     |                                                               
gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg                                                                <  1:4708106/71‑1 (MQ=255)
    cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg                                                                 >  1:3457701/1‑66 (MQ=255)
     aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                           <  1:812775/71‑1 (MQ=255)
      tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg                                                          <  1:3918818/71‑1 (MQ=255)
           tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                                                     <  1:4346774/71‑1 (MQ=255)
             ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                                                     <  1:3576495/69‑1 (MQ=255)
             ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                   <  1:2332947/71‑1 (MQ=255)
             ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                   >  1:2166381/1‑71 (MQ=255)
               ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt                                                 >  1:2197050/1‑71 (MQ=255)
                tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                >  1:3801171/1‑71 (MQ=255)
                 accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                >  1:4720228/3‑70 (MQ=255)
                 accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                >  1:1425786/3‑70 (MQ=255)
                 accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                >  1:5464106/3‑70 (MQ=255)
                 aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                >  1:5463544/1‑70 (MQ=255)
                  tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                <  1:3732014/69‑1 (MQ=255)
                   cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                                                  <  1:5044695/66‑1 (MQ=255)
                   cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCttt                                                 <  1:2727207/67‑3 (MQ=255)
                     gATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                           >  1:5291234/1‑69 (MQ=255)
                     gATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                           >  1:172019/1‑69 (MQ=255)
                      aTTGCCAAAAAGCGCAACCATCCTCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAc                                             <  1:5367204/68‑1 (MQ=255)
                      aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                          >  1:2202953/1‑71 (MQ=255)
                       ttGCCGAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                          >  1:3191869/1‑70 (MQ=255)
                       ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                          >  1:3853554/1‑70 (MQ=255)
                       ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                         <  1:4366441/71‑1 (MQ=255)
                       ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                         >  1:1663345/1‑71 (MQ=255)
                        tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                         >  1:992449/1‑70 (MQ=255)
                         gCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTa                                              >  1:3065113/1‑64 (MQ=255)
                           cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg                                     >  1:4926543/1‑71 (MQ=255)
                            aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                                    >  1:2334898/1‑71 (MQ=255)
                            aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                    >  1:1269569/1‑71 (MQ=255)
                            aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                    >  1:660056/1‑71 (MQ=255)
                            aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                    >  1:3214145/1‑71 (MQ=255)
                             aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                    >  1:435764/1‑70 (MQ=255)
                             aaaaGCGCAACCATCCCCAAGCCGACGCTATTCATAcggcgg                                                                >  1:2823040/1‑42 (MQ=255)
                               aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt                                 >  1:308777/1‑71 (MQ=255)
                               aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                           >  1:780185/1‑45 (MQ=255)
                               aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                           >  1:2292439/1‑45 (MQ=255)
                               aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt                                 >  1:1164799/1‑71 (MQ=255)
                               aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt                                 >  1:1535867/1‑71 (MQ=255)
                                aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                <  1:5631368/71‑1 (MQ=255)
                                      atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                  >  1:1354295/3‑63 (MQ=255)
                                       tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                  <  1:5340909/61‑1 (MQ=255)
                                       tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                  <  1:2660539/61‑1 (MQ=255)
                                       tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                  <  1:768040/61‑1 (MQ=255)
                                       tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                  <  1:1396186/61‑1 (MQ=255)
                                       tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTAt                                 >  1:3518308/2‑63 (MQ=255)
                                       tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                         <  1:1009023/70‑1 (MQ=255)
                                       ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                         <  1:4374643/71‑1 (MQ=255)
                                       ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                         <  1:5276981/71‑1 (MQ=255)
                                       ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                         <  1:515595/71‑1 (MQ=255)
                                       ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                         <  1:660972/71‑1 (MQ=255)
                                       ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                         <  1:2571623/71‑1 (MQ=255)
                                        cATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                                    >  1:2114441/1‑59 (MQ=255)
                                         aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGtt                                   >  1:5651686/1‑59 (MQ=255)
                                         aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGtt                                   >  1:3210049/1‑59 (MQ=255)
                                         aTCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt                       <  1:5020467/71‑1 (MQ=255)
                                         aTCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGtt                        >  1:4722256/1‑70 (MQ=255)
                                          tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                           <  1:1132009/50‑3 (MQ=255)
                                          tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                           <  1:2048407/50‑3 (MQ=255)
                                          tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                <  1:2935852/45‑1 (MQ=255)
                                          tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt                       <  1:2742562/70‑1 (MQ=255)
                                          tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                      <  1:1489890/71‑1 (MQ=255)
                                          tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                      <  1:1196356/71‑1 (MQ=255)
                                          accccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                   <  1:3960212/41‑1 (MQ=255)
                                            cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg                    <  1:2724093/71‑1 (MQ=255)
                                            cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg                    <  1:2518199/71‑1 (MQ=255)
                                            cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                     <  1:1131859/70‑1 (MQ=255)
                                            cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                     <  1:3068120/70‑1 (MQ=255)
                                            cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                     >  1:4563581/1‑70 (MQ=255)
                                             tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt                     <  1:3591591/68‑3 (MQ=255)
                                              cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                     <  1:1049063/68‑1 (MQ=255)
                                              cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg                  >  1:4692436/1‑71 (MQ=255)
                                                tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAct                                            <  1:3876431/43‑1 (MQ=255)
                                                tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa                <  1:5629547/71‑1 (MQ=255)
                                                 gCCGACGCTATTCATACGGCTGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa                <  1:469848/70‑1 (MQ=255)
                                                 gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt                     >  1:1053594/1‑63 (MQ=255)
                                                 gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                    >  1:1212457/1‑66 (MQ=255)
                                                 gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGAt               >  1:5610432/1‑71 (MQ=255)
                                                  ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt               >  1:1878292/1‑70 (MQ=255)
                                                    gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg            <  1:912649/71‑1 (MQ=255)
                                                     aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGGCGTTTCTGTGGATCTg            <  1:4249926/70‑1 (MQ=255)
                                                      cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                         <  1:3773534/56‑1 (MQ=255)
                                                      cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTggg          <  1:3254356/71‑1 (MQ=255)
                                                          aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa                                       <  1:2225296/38‑1 (MQ=255)
                                                           ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                   <  1:4987888/41‑1 (MQ=255)
                                                            tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                <  1:5308913/43‑1 (MQ=255)
                                                            tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg                 >  1:1897592/1‑58 (MQ=255)
                                                             cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg     >  1:4651877/1‑69 (MQ=255)
                                                             cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGc    >  1:876759/1‑70 (MQ=255)
                                                              aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                   <  1:4698348/38‑1 (MQ=255)
                                                              aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                   <  1:35103/38‑1 (MQ=255)
                                                              aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                   <  1:4225046/38‑1 (MQ=255)
                                                              aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc  <  1:17661/71‑1 (MQ=255)
                                                                aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                      <  1:49556/49‑1 (MQ=255)
                                                                aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg            >  1:4947383/1‑59 (MQ=255)
                                                                 cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                           >  1:3358285/1‑43 (MQ=255)
                                                                 cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                           >  1:2241659/1‑43 (MQ=255)
                                                                 cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                      >  1:3899144/1‑48 (MQ=255)
                                                                 cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                      >  1:944097/1‑48 (MQ=255)
                                                                   gcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGcca       >  1:4891553/1‑59 (MQ=255)
                                                                     |                                                               
GATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTC  >  minE/2569802‑2569934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A