Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,569,883 C→T 35.4% S51S (AGC→AGT)  yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,569,8830CT35.4% 149.2 / 130.4 147S51S (AGC→AGT) yibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (47/48);  new base T (25/27);  total (72/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 149 total aligned reads displayed.

TCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGG  >  minE/2569813‑2569951
                                                                      |                                                                    
tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                                                                      <  1:4346774/71‑1 (MQ=255)
  ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                                    >  1:2166381/1‑71 (MQ=255)
  ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                                    <  1:2332947/71‑1 (MQ=255)
   cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                                                                   >  1:4444284/1‑71 (MQ=255)
    ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt                                                                  >  1:2197050/1‑71 (MQ=255)
     tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                                 >  1:3100864/1‑71 (MQ=255)
      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                                 >  1:1425786/3‑70 (MQ=255)
      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                                 >  1:3829765/3‑70 (MQ=255)
      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                                 >  1:4671916/3‑70 (MQ=255)
      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                                 >  1:4720228/3‑70 (MQ=255)
      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                                 >  1:5464106/3‑70 (MQ=255)
      aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                                 >  1:5463544/1‑70 (MQ=255)
      aTCTGATTGCCAAAAAGCGCAACCATCCCAATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                                 >  1:1190558/1‑70 (MQ=255)
       tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                                 <  1:3732014/69‑1 (MQ=255)
       ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                                 <  1:3537745/68‑1 (MQ=255)
        cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                                                                   <  1:5044695/66‑1 (MQ=255)
        cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc                                                              <  1:1573822/71‑1 (MQ=255)
          gATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                                            >  1:172019/1‑69 (MQ=255)
           aTTGCCAAAAAGCGCAACCATCCTCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAc                                                              <  1:5367204/68‑1 (MQ=255)
           aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                                           >  1:2202953/1‑71 (MQ=255)
            ttGCCGAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                                           >  1:3191869/1‑70 (MQ=255)
            ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                                           >  1:3853554/1‑70 (MQ=255)
            ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                                          <  1:4366441/71‑1 (MQ=255)
             tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                                          >  1:992449/1‑70 (MQ=255)
                cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg                                                      >  1:4926543/1‑71 (MQ=255)
                 aaaaaaCGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                                                     >  1:2334898/1‑71 (MQ=255)
                 aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                                     >  1:3214145/1‑71 (MQ=255)
                 aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                                     >  1:660056/1‑71 (MQ=255)
                 aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                                     >  1:1269569/1‑71 (MQ=255)
                  aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                                     >  1:435764/1‑70 (MQ=255)
                  aaaaACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                                                     >  1:4942626/1‑70 (MQ=255)
                    aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt                                                  >  1:308777/1‑71 (MQ=255)
                    aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt                                                  >  1:1535867/1‑71 (MQ=255)
                     aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                                 <  1:5631368/71‑1 (MQ=255)
                     aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                                 <  1:5297502/71‑1 (MQ=255)
                       cgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTg                                               >  1:4147305/1‑71 (MQ=255)
                          aaCCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                                 <  1:3414335/66‑1 (MQ=255)
                           atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                                   >  1:1354295/3‑63 (MQ=255)
                            tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                                   <  1:2660539/61‑1 (MQ=255)
                            tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                                   <  1:768040/61‑1 (MQ=255)
                            tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTAt                                                  >  1:3518308/2‑63 (MQ=255)
                            tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                                          <  1:1009023/70‑1 (MQ=255)
                            ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                          <  1:660972/71‑1 (MQ=255)
                            ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                          <  1:515595/71‑1 (MQ=255)
                            ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                          <  1:2571623/71‑1 (MQ=255)
                            ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                          <  1:5276981/71‑1 (MQ=255)
                              aTCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGtt                                         >  1:4722256/1‑70 (MQ=255)
                               tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                                            <  1:2048407/50‑3 (MQ=255)
                               tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                                            <  1:1132009/50‑3 (MQ=255)
                               tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                                 <  1:2935852/45‑1 (MQ=255)
                               tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGttt                                        <  1:2742562/70‑1 (MQ=255)
                               accccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                                    <  1:3960212/41‑1 (MQ=255)
                                 ctcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                                     >  1:2797549/3‑71 (MQ=255)
                                 cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg                                     <  1:2518199/71‑1 (MQ=255)
                                 cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg                                     <  1:2724093/71‑1 (MQ=255)
                                 cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                                      <  1:3899699/70‑1 (MQ=255)
                                 cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                                      <  1:3068120/70‑1 (MQ=255)
                                 cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                                      <  1:1131859/70‑1 (MQ=255)
                                  tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt                                      <  1:3591591/68‑3 (MQ=255)
                                  ccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                                    <  1:3216579/55‑1 (MQ=255)
                                   cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                                      <  1:1049063/68‑1 (MQ=255)
                                   cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg                                   >  1:2941559/1‑71 (MQ=255)
                                    aTGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                                     <  1:5633290/68‑1 (MQ=255)
                                     tGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGa                                 <  1:1399860/71‑1 (MQ=255)
                                      gCCGACGCTATTCATACGGCTGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa                                 <  1:469848/70‑1 (MQ=255)
                                      gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt                                      >  1:1053594/1‑63 (MQ=255)
                                      gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                                     >  1:1212457/1‑66 (MQ=255)
                                      gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                                     >  1:5564650/1‑66 (MQ=255)
                                      gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGAt                                >  1:5610432/1‑71 (MQ=255)
                                       ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt                                >  1:1878292/1‑70 (MQ=255)
                                         gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg                             <  1:912649/71‑1 (MQ=255)
                                          aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGGCGTTTCTGTGGATCTg                             <  1:4249926/70‑1 (MQ=255)
                                           cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                                          <  1:3773534/56‑1 (MQ=255)
                                               aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa                                                        <  1:2225296/38‑1 (MQ=255)
                                                ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                                    <  1:4987888/41‑1 (MQ=255)
                                                 tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                                 <  1:5308913/43‑1 (MQ=255)
                                                 tCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg                                  >  1:1897592/1‑58 (MQ=255)
                                                  cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg                      >  1:4651877/1‑69 (MQ=255)
                                                  cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGc                     >  1:876759/1‑70 (MQ=255)
                                                   aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                                    <  1:4698348/38‑1 (MQ=255)
                                                   aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                                    <  1:4225046/38‑1 (MQ=255)
                                                   aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc                   <  1:17661/71‑1 (MQ=255)
                                                     aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg                             >  1:4947383/1‑59 (MQ=255)
                                                      cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                                            >  1:5406819/1‑43 (MQ=255)
                                                      cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                                            >  1:3358285/1‑43 (MQ=255)
                                                      cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                                            >  1:2241659/1‑43 (MQ=255)
                                                      cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                                       >  1:3899144/1‑48 (MQ=255)
                                                      cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                                       >  1:944097/1‑48 (MQ=255)
                                                        gcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGcca                        >  1:4891553/1‑59 (MQ=255)
                                                            gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg                                   <  1:4017103/44‑1 (MQ=255)
                                                            gttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg                                   <  1:1668426/44‑1 (MQ=255)
                                                            gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGa                        >  1:1167625/1‑57 (MQ=255)
                                                            gCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGAGCGACg                      <  1:734327/59‑1 (MQ=255)
                                                             ttGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg                                   <  1:1205255/44‑1 (MQ=255)
                                                               gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTgg                                  <  1:4455985/44‑1 (MQ=255)
                                                               gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg       >  1:5049589/1‑71 (MQ=255)
                                                               gggTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAg       >  1:2167260/1‑71 (MQ=255)
                                                                   gAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg   >  1:2055722/1‑71 (MQ=255)
                                                                    aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg   <  1:4988941/70‑1 (MQ=255)
                                                                    aGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTg   <  1:179309/70‑1 (MQ=255)
                                                                      |                                                                    
TCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCGGAGCGTGG  >  minE/2569813‑2569951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A