Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,570,785 T→G 21.4% V230V (GTT→GTG)  yibH → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,570,7850TG21.4% 154.7 / 55.7 98V230V (GTT→GTG) yibHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/37);  new base G (17/4);  total (57/41)
Fisher's exact test for biased strand distribution p-value = 2.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATC  >  minE/2570716‑2570854
                                                                     |                                                                     
agCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTa                                                                      >  1:4617417/1‑71 (MQ=255)
 gcgcAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTAc                                                                     >  1:217300/1‑71 (MQ=255)
    cAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACt                                                                    >  1:2620716/1‑69 (MQ=255)
      aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgt                                                                <  1:1579372/71‑2 (MQ=255)
      aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                                >  1:5504789/1‑71 (MQ=255)
      aCTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                                >  1:766332/1‑71 (MQ=255)
          aCTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACt                                                            >  1:129133/1‑71 (MQ=255)
           cTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTa                                                              <  1:1242373/68‑1 (MQ=255)
              aaGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATc                                                        >  1:5325897/1‑71 (MQ=255)
              aaGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGAtt                                                        >  1:5628654/1‑70 (MQ=255)
              aaGCAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGAtt                                                        >  1:2660324/1‑70 (MQ=255)
                 cAAAATATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCg                                                      <  1:930966/70‑1 (MQ=255)
                  aaaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGAc                                                                     >  1:4144447/1‑54 (MQ=255)
                  aaaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCgt                                                     <  1:212453/70‑2 (MQ=255)
                   aaaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGtt                                                                       <  1:3532462/51‑1 (MQ=255)
                   aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                   >  1:1253642/1‑71 (MQ=255)
                   aaaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtcc                                                   <  1:3208415/71‑1 (MQ=255)
                    aaTATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCa                                                  <  1:1698509/71‑1 (MQ=255)
                    aaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTa                                                              >  1:3456040/1‑59 (MQ=255)
                    aaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtccg                                                  >  1:2880584/1‑70 (MQ=255)
                    aaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtccg                                                  >  1:2733471/1‑70 (MQ=255)
                    aaTATAACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGtccg                                                  >  1:2610651/1‑70 (MQ=255)
                     atatAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAg                                                 >  1:1906756/1‑71 (MQ=255)
                      tataACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAgg                                                >  1:3649775/1‑71 (MQ=255)
                        taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                              >  1:1397815/1‑71 (MQ=255)
                        taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                              <  1:3749721/71‑1 (MQ=255)
                        taACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTa                                              >  1:4119278/1‑71 (MQ=255)
                        taACCTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTa                                              >  1:5628176/1‑71 (MQ=255)
                          aCCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                            <  1:2085988/71‑1 (MQ=255)
                           ccTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCgtc                                                    >  1:3467639/1‑60 (MQ=255)
                            cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                                <  1:449845/49‑1 (MQ=255)
                            cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                                <  1:2222215/49‑1 (MQ=255)
                            cTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAgg                                                                <  1:2274309/49‑1 (MQ=255)
                            cTTGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTa                                          >  1:1271195/1‑71 (MQ=255)
                             ttGAGCAGACGATAGTGCGTGCGCCGAGCGATGGTTATGTGACCCAGGTACTGa                                                          >  1:562770/1‑54 (MQ=255)
                              tGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACg                                        >  1:2174325/1‑71 (MQ=255)
                               gAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGa                                                          >  1:2387129/1‑52 (MQ=255)
                                aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                       <  1:2295133/70‑1 (MQ=255)
                                aGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGc                                       <  1:1028155/70‑1 (MQ=255)
                                   aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                   <  1:428766/71‑1 (MQ=255)
                                   aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                   <  1:3218372/71‑1 (MQ=255)
                                   aGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCt                                   <  1:3225794/71‑1 (MQ=255)
                                    gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGc                                                     >  1:359309/1‑52 (MQ=255)
                                    gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACa                                            <  1:1560887/61‑1 (MQ=255)
                                    gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                  <  1:2670348/71‑1 (MQ=255)
                                    gACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                  >  1:3098634/1‑71 (MQ=255)
                                      cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                    >  1:3899776/1‑67 (MQ=255)
                                      cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                    >  1:5506930/1‑67 (MQ=255)
                                      cTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGc                                    >  1:917187/1‑67 (MQ=255)
                                         tCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCtt                              <  1:124460/70‑1 (MQ=255)
                                           aTTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                           >  1:2726988/1‑71 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:3667828/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:96965/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:2016074/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:3303062/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:1277911/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:3065483/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:4652072/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:5155473/71‑1 (MQ=255)
                                            ttCGCGCGCCGAGCAAAGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          <  1:1593534/71‑1 (MQ=255)
                                             tCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                         >  1:4405786/1‑71 (MQ=255)
                                              cgcgcgCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCg                          >  1:4819656/1‑69 (MQ=255)
                                                 gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                             >  1:974992/1‑47 (MQ=255)
                                                 gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                             >  1:3886713/1‑47 (MQ=255)
                                                 gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                             >  1:2029958/1‑47 (MQ=255)
                                                 gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                             >  1:2616414/1‑47 (MQ=255)
                                                 gcgcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTAc                                             >  1:3157193/1‑47 (MQ=255)
                                                  cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                    >  1:2187208/1‑71 (MQ=255)
                                                  cgcCGAGCGATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGt                    >  1:4854762/1‑71 (MQ=255)
                                                  cgcCGAGCCATGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTatg                                        >  1:5221741/1‑49 (MQ=255)
                                                   gcagAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                   >  1:5095792/4‑71 (MQ=255)
                                                   gcCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                   >  1:5027913/1‑71 (MQ=255)
                                                      gAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                <  1:4523482/71‑1 (MQ=255)
                                                      gAGCAATGGCTACGTTACTCAGGTACTGAACCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTcc                  >  1:2462052/1‑69 (MQ=255)
                                                       aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                  >  1:3905850/1‑52 (MQ=255)
                                                       aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                  >  1:1658442/1‑52 (MQ=255)
                                                       aGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTg                                  >  1:13360/1‑52 (MQ=255)
                                                        gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                <  1:2791965/69‑1 (MQ=255)
                                                        gCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCgg                <  1:3771478/69‑1 (MQ=255)
                                                           aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg           <  1:4950043/71‑1 (MQ=255)
                                                           aTGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATg           >  1:544441/1‑71 (MQ=255)
                                                           aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                   <  1:5461260/63‑1 (MQ=255)
                                                           aTGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                   <  1:2249279/63‑1 (MQ=255)
                                                            tGGTTATGTGACCCAGGTACTGATTCGTCCGGGTACCTATGCCGCGTCGCTGCCGCTACGTCCGGTGATgg          >  1:622311/1‑71 (MQ=255)
                                                            tGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGa             <  1:198856/68‑1 (MQ=255)
                                                             ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                 >  1:4733665/1‑63 (MQ=255)
                                                             ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCg                 >  1:951436/1‑63 (MQ=255)
                                                             ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATgg          >  1:2486290/1‑70 (MQ=255)
                                                             ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt         >  1:5129572/1‑71 (MQ=255)
                                                             ggCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGt         >  1:4296935/1‑71 (MQ=255)
                                                              gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc        >  1:3392954/1‑71 (MQ=255)
                                                              gCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTc        <  1:2328269/71‑1 (MQ=255)
                                                               cTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCt       <  1:4172809/71‑1 (MQ=255)
                                                                  cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCtt      <  1:1319461/69‑1 (MQ=255)
                                                                  cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTc     <  1:4657486/70‑1 (MQ=255)
                                                                  cGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCa    >  1:2203160/1‑71 (MQ=255)
                                                                   gTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGcc                           <  1:4329799/47‑1 (MQ=255)
                                                                    ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGc                         <  1:3557122/48‑1 (MQ=255)
                                                                    ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTc                   <  1:2937265/54‑1 (MQ=255)
                                                                    ttACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATc  >  1:508147/1‑71 (MQ=255)
                                                                     |                                                                     
AGCGCAACTTACTGAAGCAAAATATAACCTTGAGCAGACTGTCATTCGCGCGCCGAGCAATGGCTACGTTACTCAGGTACTGATCCGCCCAGGTACATACGCAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATC  >  minE/2570716‑2570854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A