Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,326 A→C 33.0% R424R (CGT→CGG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,3260AC33.0% 88.5 / 108.8 112R424R (CGT→CGG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (35/40);  new base C (19/18);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTA  >  minE/2595256‑2595394
                                                                      |                                                                    
tGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAgagaga                                                                      <  1:3901486/71‑1 (MQ=255)
  ccGCAGGCGGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACg                                                                    <  1:4105189/71‑1 (MQ=255)
   cacAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCgc                                                                   <  1:3027353/69‑2 (MQ=255)
   cacAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCgc                                                                   <  1:3848867/69‑2 (MQ=255)
   cacAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCgc                                                                   >  1:5072877/3‑70 (MQ=255)
   cacAGGCCGGGTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCgc                                                                   >  1:3576424/3‑70 (MQ=255)
     cAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAg                                                                 <  1:2406121/71‑1 (MQ=255)
      aGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgc                                                                >  1:61564/1‑71 (MQ=255)
            ggTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                          <  1:2453903/71‑1 (MQ=255)
              ttGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCgcc                                                                  <  1:1653857/61‑3 (MQ=255)
              ttGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                        <  1:340183/71‑1 (MQ=255)
               tgtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                        <  1:4702643/70‑1 (MQ=255)
                  gATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggc                                                     >  1:4396535/1‑70 (MQ=255)
                   aTAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAg                                                           >  1:3306303/1‑63 (MQ=255)
                    tAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccag                                                  <  1:2410854/71‑1 (MQ=255)
                    tAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagcca                                                   <  1:574231/70‑1 (MQ=255)
                     agaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAg                                                           <  1:5335149/59‑1 (MQ=255)
                     aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3469632/1‑71 (MQ=255)
                     aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3462899/1‑71 (MQ=255)
                     aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3958727/1‑71 (MQ=255)
                      gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                 <  1:3367669/69‑2 (MQ=255)
                       aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGTGCCAGCGCAGCCAGCGt                                               >  1:4839064/1‑71 (MQ=255)
                       aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                               <  1:5387995/71‑1 (MQ=255)
                       aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                         >  1:939264/1‑61 (MQ=255)
                       aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                         >  1:544843/1‑61 (MQ=255)
                        tGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                              <  1:5228961/71‑1 (MQ=255)
                         ggTAATGTTGCTCATTTCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                             >  1:4247100/1‑69 (MQ=255)
                         ggTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                             >  1:4797135/1‑69 (MQ=255)
                         ggTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                             >  1:2836887/1‑69 (MQ=255)
                              tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     <  1:155170/58‑1 (MQ=255)
                               gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTa                                                                  >  1:2370715/1‑44 (MQ=255)
                               gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:2162843/1‑57 (MQ=255)
                               gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:2083160/1‑57 (MQ=255)
                               gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:4469462/1‑57 (MQ=255)
                                ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGGGCAGCCAGCGTCACAAACAg                                      >  1:5265201/1‑71 (MQ=255)
                                ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:3899196/70‑1 (MQ=255)
                                ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:4281743/70‑1 (MQ=255)
                                ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:5067645/70‑1 (MQ=255)
                                ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:1086304/70‑1 (MQ=255)
                                   cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGcc                                                             >  1:4304109/1‑45 (MQ=255)
                                   cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:2572734/71‑1 (MQ=255)
                                   cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:844464/71‑1 (MQ=255)
                                   cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                    <  1:1061871/70‑1 (MQ=255)
                                     cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcaccg                                 >  1:4169565/1‑69 (MQ=255)
                                      atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:2809995/64‑1 (MQ=255)
                                      atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:5688686/64‑1 (MQ=255)
                                      atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:541780/64‑1 (MQ=255)
                                      atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                >  1:4622462/1‑71 (MQ=255)
                                       tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgc                                                          <  1:5650733/44‑1 (MQ=255)
                                       tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:5087646/51‑1 (MQ=255)
                                       tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:4603532/51‑1 (MQ=255)
                                       tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:4323806/51‑1 (MQ=255)
                                        atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   >  1:2786411/1‑50 (MQ=255)
                                         tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                              >  1:2403905/1‑54 (MQ=255)
                                         tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                              <  1:1859326/70‑1 (MQ=255)
                                         tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                              <  1:1831062/70‑1 (MQ=255)
                                         tCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                 >  1:124132/1‑50 (MQ=255)
                                           aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                           <  1:977500/71‑1 (MQ=255)
                                             tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                    <  1:3053832/60‑1 (MQ=255)
                                               tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                           <  1:2242025/51‑1 (MQ=255)
                                               tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:3134278/1‑56 (MQ=255)
                                               tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:705235/1‑56 (MQ=255)
                                               tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                             <  1:3355540/65‑1 (MQ=255)
                                               tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                       >  1:65953/1‑71 (MQ=255)
                                               tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccag                                                  <  1:2731350/44‑1 (MQ=12)
                                               tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCAccggcaca                            >  1:3592595/1‑64 (MQ=255)
                                               tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                       >  1:1518678/1‑71 (MQ=255)
                                               tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                       >  1:1160607/1‑71 (MQ=255)
                                                  aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                     >  1:1664135/2‑70 (MQ=255)
                                                   aTTACAAAGTGACAGAGAGCCGCCACGCCAgcgcg                                                       <  1:919605/35‑2 (MQ=12)
                                                   aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    >  1:4377325/1‑69 (MQ=255)
                                                   aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    >  1:1737157/1‑69 (MQ=255)
                                                   aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    <  1:3521973/70‑2 (MQ=255)
                                                   aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                    <  1:4851837/70‑1 (MQ=255)
                                                   aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                   <  1:5540100/71‑1 (MQ=255)
                                                    ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:4860878/50‑1 (MQ=255)
                                                    ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                  <  1:308626/71‑1 (MQ=255)
                                                     tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                 >  1:5577178/1‑71 (MQ=255)
                                                      aCAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAAc                                        >  1:1909327/1‑47 (MQ=255)
                                                      aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                     >  1:4675637/1‑66 (MQ=255)
                                                      aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                >  1:2982070/1‑71 (MQ=255)
                                                      aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                >  1:2094823/1‑71 (MQ=255)
                                                       cAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAtgc               <  1:3516961/71‑3 (MQ=255)
                                                       cAAAGTGAAAGAGAGACGTAGCGCCAGCTcagcca                                                   >  1:1783513/1‑35 (MQ=255)
                                                       cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc               >  1:1603561/1‑71 (MQ=255)
                                                        aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:1261978/50‑1 (MQ=255)
                                                        aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:4110322/50‑1 (MQ=255)
                                                         aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                            >  1:2154353/1‑56 (MQ=255)
                                                         aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                            >  1:3923040/1‑56 (MQ=255)
                                                          aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:4786190/71‑1 (MQ=255)
                                                          aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:4669639/71‑1 (MQ=255)
                                                           gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg            >  1:5242204/1‑68 (MQ=255)
                                                           gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            >  1:1153276/1‑70 (MQ=255)
                                                           gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt           <  1:969119/71‑1 (MQ=255)
                                                                agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                   >  1:2000060/1‑58 (MQ=255)
                                                                   gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg    >  1:1827920/1‑70 (MQ=255)
                                                                   gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt   <  1:1696360/71‑1 (MQ=255)
                                                                   gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt   <  1:5453997/71‑1 (MQ=255)
                                                                    agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa  <  1:1321587/71‑1 (MQ=255)
                                                                    agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa  <  1:5410098/71‑1 (MQ=255)
                                                                      |                                                                    
TGCCGCAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTA  >  minE/2595256‑2595394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A