Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,330 A→C 28.3% L423R (CTA→CGA) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,3300AC28.3% 128.4 / 92.5 113L423R (CTA→CGA) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (36/45);  new base C (17/15);  total (53/60)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 117 total aligned reads displayed.

CAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTA  >  minE/2595261‑2595394
                                                                     |                                                                
cAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAg                                                                 <  1:2406121/71‑1 (MQ=255)
 aGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgc                                                                >  1:61564/1‑71 (MQ=255)
   gCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAgcgc                                                              <  1:455331/71‑1 (MQ=255)
       ggTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                          <  1:2453903/71‑1 (MQ=255)
        gTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                          <  1:3826773/70‑1 (MQ=255)
         ttGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                        <  1:340183/71‑1 (MQ=255)
          tgtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                        <  1:4702643/70‑1 (MQ=255)
           gtgATAAATGCTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGca                                                       <  1:4087390/70‑1 (MQ=255)
             gATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggc                                                     >  1:4396535/1‑70 (MQ=255)
               tAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagcca                                                   <  1:574231/70‑1 (MQ=255)
                agaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAg                                                           <  1:5335149/59‑1 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3469632/1‑71 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                 >  1:3462899/1‑71 (MQ=255)
                 gaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                 <  1:3367669/69‑2 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                               <  1:5387995/71‑1 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                               >  1:3468783/1‑71 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                         >  1:939264/1‑61 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                         >  1:544843/1‑61 (MQ=255)
                    ggTAATGTTGCTCATTTCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                             >  1:4247100/1‑69 (MQ=255)
                    ggTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                             >  1:4797135/1‑69 (MQ=255)
                       aaTGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                           >  1:1251148/1‑70 (MQ=255)
                         tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     <  1:155170/58‑1 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTa                                                                  >  1:2370715/1‑44 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:2083160/1‑57 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:2162843/1‑57 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                     >  1:4469462/1‑57 (MQ=255)
                           ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGGGCAGCCAGCGTCACAAACAg                                      >  1:5265201/1‑71 (MQ=255)
                           ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:287879/1‑71 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:3899196/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:4281743/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:5067645/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                       <  1:1086304/70‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:2572734/71‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                    <  1:1061871/70‑1 (MQ=255)
                                cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcaccg                                 >  1:4169565/1‑69 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:2809995/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:2459377/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:5688686/64‑1 (MQ=255)
                                  tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgc                                                          <  1:5650733/44‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:5087646/51‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:4603532/51‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   <  1:4323806/51‑1 (MQ=255)
                                   atCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       >  1:2065172/1‑62 (MQ=255)
                                   atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                   >  1:2786411/1‑50 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                              >  1:2403905/1‑54 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                             >  1:4892038/1‑71 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                              <  1:1859326/70‑1 (MQ=255)
                                    tCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                 >  1:124132/1‑50 (MQ=255)
                                      aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                           <  1:977500/71‑1 (MQ=255)
                                        tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                    <  1:3053832/60‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                           <  1:2242025/51‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa                                         >  1:4709551/1‑53 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:705235/1‑56 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                      >  1:3134278/1‑56 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                             <  1:4290259/65‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                             <  1:3355540/65‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                       >  1:65953/1‑71 (MQ=255)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccag                                                  <  1:2731350/44‑1 (MQ=12)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCAccggcaca                            >  1:3592595/1‑64 (MQ=255)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                       >  1:1160607/1‑71 (MQ=255)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                       >  1:1518678/1‑71 (MQ=255)
                                             aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                     >  1:1664135/2‑70 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAgcgcg                                                       <  1:919605/35‑2 (MQ=12)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    >  1:1737157/1‑69 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    >  1:4377325/1‑69 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                    <  1:3521973/70‑2 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                      <  1:2284194/68‑1 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                    <  1:4851837/70‑1 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                   <  1:5540100/71‑1 (MQ=255)
                                               ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                       <  1:4860878/50‑1 (MQ=255)
                                               ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                  <  1:308626/71‑1 (MQ=255)
                                                tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                 >  1:5577178/1‑71 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAAc                                        >  1:1909327/1‑47 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                     >  1:4675637/1‑66 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                >  1:2982070/1‑71 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                >  1:2094823/1‑71 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCTcagcca                                                   >  1:1783513/1‑35 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc               >  1:1603561/1‑71 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc               <  1:543292/71‑1 (MQ=255)
                                                   aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                   <  1:4110322/50‑1 (MQ=255)
                                                    aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                            >  1:3923040/1‑56 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:4786190/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:3202795/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:1826622/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            <  1:4669639/71‑1 (MQ=255)
                                                      gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg            >  1:5242204/1‑68 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            >  1:1153276/1‑70 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg            >  1:5256164/1‑70 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt           <  1:969119/71‑1 (MQ=255)
                                                           agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                   >  1:2000060/1‑58 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg    >  1:1827920/1‑70 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg    >  1:825321/1‑70 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt   <  1:5453997/71‑1 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt   <  1:163546/71‑1 (MQ=255)
                                                               agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa  <  1:5410098/71‑1 (MQ=255)
                                                               agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa  <  1:1321587/71‑1 (MQ=255)
                                                                    tAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggc                               <  1:1791825/37‑1 (MQ=255)
                                                                     aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                 >  1:1784388/1‑50 (MQ=255)
                                                                     aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                 >  1:5171048/1‑50 (MQ=255)
                                                                     aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                 >  1:993475/1‑50 (MQ=255)
                                                                     |                                                                
CAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTA  >  minE/2595261‑2595394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A