Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I0 R1 2892 75.3 5698161 66.1% 3766484 59.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,367 G→C 29.7% L411V (CTG→GTG)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,3670GC29.7% 148.1 / 77.1 101L411V (CTG→GTG) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (39/32);  new base C (17/13);  total (56/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 118 total aligned reads displayed.

TCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGC  >  minE/2595297‑2595437
                                                                      |                                                                      
tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                                                        >  1:4892038/1‑71 (MQ=255)
  aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                                                                      <  1:977500/71‑1 (MQ=255)
      tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                                                        <  1:3355540/65‑1 (MQ=255)
      tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                                  >  1:65953/1‑71 (MQ=255)
      tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                                                                  >  1:1160607/1‑71 (MQ=255)
      tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                                                                  >  1:1518678/1‑71 (MQ=255)
         aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                                                                >  1:1664135/2‑70 (MQ=255)
          aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:3521973/70‑2 (MQ=255)
          aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                                               >  1:4377325/1‑69 (MQ=255)
          aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                                               >  1:1737157/1‑69 (MQ=255)
          aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                                                                 <  1:2284194/68‑1 (MQ=255)
          aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                                                               <  1:4851837/70‑1 (MQ=255)
          aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                                              <  1:5540100/71‑1 (MQ=255)
            tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                                                            >  1:5577178/1‑71 (MQ=255)
            tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                                                        <  1:5680226/59‑1 (MQ=255)
             aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                                                >  1:4675637/1‑66 (MQ=255)
             aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                                           >  1:2982070/1‑71 (MQ=255)
             aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                                           >  1:2094823/1‑71 (MQ=255)
              cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                          <  1:543292/71‑1 (MQ=255)
              cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                          >  1:1603561/1‑71 (MQ=255)
                aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                                                                       >  1:3923040/1‑56 (MQ=255)
                aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                                                                       >  1:2154353/1‑56 (MQ=255)
                 aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:4786190/71‑1 (MQ=255)
                 aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:4669639/71‑1 (MQ=255)
                 aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:1826622/71‑1 (MQ=255)
                 aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:3202795/71‑1 (MQ=255)
                  gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       >  1:5256164/1‑70 (MQ=255)
                  gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       >  1:1153276/1‑70 (MQ=255)
                  gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt                                                      <  1:969119/71‑1 (MQ=255)
                       agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                                              >  1:2000060/1‑58 (MQ=255)
                          gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                                               >  1:825321/1‑70 (MQ=255)
                          gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                                               >  1:1827920/1‑70 (MQ=255)
                          gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                              <  1:1696360/71‑1 (MQ=255)
                          gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                              <  1:163546/71‑1 (MQ=255)
                           agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                             <  1:1321587/71‑1 (MQ=255)
                           agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                             <  1:5410098/71‑1 (MQ=255)
                                 cacgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcc                                       >  1:5377867/3‑71 (MQ=255)
                                 aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                                                            >  1:1784388/1‑50 (MQ=255)
                                 aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                                                            >  1:5171048/1‑50 (MQ=255)
                                  gcgcCCGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                                  >  1:1979192/1‑43 (MQ=255)
                                  gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                                  >  1:83857/1‑43 (MQ=255)
                                  gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                                                >  1:3131452/1‑45 (MQ=255)
                                  gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                      >  1:5127962/1‑71 (MQ=255)
                                  acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                      >  1:3025161/2‑71 (MQ=255)
                                  acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                      >  1:2590656/2‑71 (MQ=255)
                                   cgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                     <  1:3471919/71‑1 (MQ=255)
                                     ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg                                   >  1:4473558/1‑71 (MQ=255)
                                       aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                                 <  1:130726/71‑1 (MQ=255)
                                        gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                     <  1:4704743/66‑1 (MQ=255)
                                        gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                                 >  1:2839713/1‑70 (MQ=255)
                                         cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:1243161/71‑1 (MQ=255)
                                         cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:29933/71‑1 (MQ=255)
                                         cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:513441/71‑1 (MQ=255)
                                           cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt                                                        <  1:2041747/44‑1 (MQ=255)
                                           cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               >  1:3649646/1‑69 (MQ=255)
                                           cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               >  1:5070701/1‑69 (MQ=255)
                                            ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg                            >  1:3870623/2‑69 (MQ=255)
                                             gccagaGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:257711/35‑2 (MQ=12)
                                             gccagaGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:2503828/35‑2 (MQ=12)
                                             gccagaGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:584133/35‑2 (MQ=12)
                                               cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGCTAGTCa                         <  1:5555/71‑1 (MQ=255)
                                               cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                         <  1:214073/71‑1 (MQ=255)
                                                agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                         <  1:1674147/70‑1 (MQ=255)
                                                agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                         >  1:5646237/1‑70 (MQ=255)
                                                  cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                      <  1:4148128/71‑1 (MQ=255)
                                                  cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                      <  1:614030/71‑1 (MQ=255)
                                                   gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                      <  1:2985680/70‑1 (MQ=255)
                                                    tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTaa                                            >  1:60203/3‑47 (MQ=16)
                                                    tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                     <  1:4123704/52‑1 (MQ=255)
                                                    tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt                    <  1:1203956/69‑2 (MQ=255)
                                                    tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:4270512/60‑1 (MQ=255)
                                                    tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                    <  1:1791542/71‑1 (MQ=255)
                                                     cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                          >  1:5575767/1‑64 (MQ=255)
                                                      acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                  <  1:3852905/71‑1 (MQ=255)
                                                      acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt                   <  1:2152288/70‑3 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                >  1:1231302/1‑41 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                >  1:1390253/1‑41 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                >  1:2766781/1‑41 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                       >  1:24804/1‑50 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg                                   >  1:2919375/1‑54 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                  >  1:4167808/1‑71 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                  >  1:4352398/1‑71 (MQ=255)
                                                      acaAACAGCACAGGCAGGGTCATGATAATCCCTGAACGGAAGTAATATcc                                       >  1:5395728/1‑50 (MQ=255)
                                                      acaAACAGCAAAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                  >  1:2334813/1‑71 (MQ=255)
                                                       caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc                 <  1:3068514/71‑1 (MQ=255)
                                                        aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                >  1:4119604/1‑71 (MQ=255)
                                                        aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                >  1:760969/1‑71 (MQ=255)
                                                         aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                       >  1:4917045/1‑47 (MQ=255)
                                                         aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTg               >  1:1783083/1‑71 (MQ=255)
                                                           cagcagAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc              >  1:5555800/1‑70 (MQ=255)
                                                            aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg             >  1:4332141/1‑68 (MQ=255)
                                                               acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATtcttct                >  1:34713/1‑64 (MQ=255)
                                                                  ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc      <  1:1130225/71‑1 (MQ=255)
                                                                  ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc      <  1:4430395/71‑1 (MQ=255)
                                                                   gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg     <  1:5252685/71‑1 (MQ=255)
                                                                    caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt    <  1:1486229/71‑1 (MQ=255)
                                                                    caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt    <  1:4120927/71‑1 (MQ=255)
                                                                     aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                       >  1:3604778/1‑51 (MQ=255)
                                                                     aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                       >  1:2803758/1‑51 (MQ=255)
                                                                      gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc  <  1:1524422/71‑1 (MQ=255)
                                                                      |                                                                      
TCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGC  >  minE/2595297‑2595437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A